Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber‐Based Nerve Guidance Conduit to Promote Peripheral Nerve Repair
Abstract Peripheral nerve injury is a serious medical problem with limited surgical and clinical treatment options. It is of great significance to integrate multiple guidance cues in one platform of nerve guidance conduits (NGCs) to promote axonal elongation and functional recovery. Here, a multi‐fu...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-02-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202203296 |
| _version_ | 1827820369858265088 |
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| author | Binghui Jin Yiling Yu Chenghao Lou Xiaodi Zhang Bowen Gong Jinghao Chen Xiangxiang Chen Zihan Zhou Liqun Zhang Jian Xiao Jiajia Xue |
| author_facet | Binghui Jin Yiling Yu Chenghao Lou Xiaodi Zhang Bowen Gong Jinghao Chen Xiangxiang Chen Zihan Zhou Liqun Zhang Jian Xiao Jiajia Xue |
| author_sort | Binghui Jin |
| collection | DOAJ |
| description | Abstract Peripheral nerve injury is a serious medical problem with limited surgical and clinical treatment options. It is of great significance to integrate multiple guidance cues in one platform of nerve guidance conduits (NGCs) to promote axonal elongation and functional recovery. Here, a multi‐functional NGC is constructed to promote nerve regeneration by combining ordered topological structure, density gradient of biomacromolecular nanoparticles, and controlled delivery of biological effectors to provide the topographical, haptotactic, and biological cues, respectively. On the surface of aligned polycaprolactone nanofibers, a density gradient of bioactive nanoparticles capable of delivering recombinant human acidic fibroblast growth factor is deposited. On the graded scaffold, the proliferation of Schwann cells is promoted, and the directional extension of neurites from both PC12 cells and dorsal root ganglions is improved in the direction of increasing particle density. After being implanted in vivo for 6 and 12 weeks to repair a 10‐mm rat sciatic nerve defect, the NGC promotes axonal elongation and remyelination, achieving the regeneration of the nerve not only in anatomical structure but also in functional recovery. Taken together, the NGC provides a favorable microenvironment for peripheral nerve regeneration and holds great promise for realizing nerve repair with an efficacy close to autograft. |
| first_indexed | 2024-03-12T01:26:54Z |
| format | Article |
| id | doaj.art-0c9ded6c9e334d24af167dc6cc7b0a01 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-12T01:26:54Z |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-0c9ded6c9e334d24af167dc6cc7b0a012023-09-12T14:40:47ZengWileyAdvanced Science2198-38442023-02-01104n/an/a10.1002/advs.202203296Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber‐Based Nerve Guidance Conduit to Promote Peripheral Nerve RepairBinghui Jin0Yiling Yu1Chenghao Lou2Xiaodi Zhang3Bowen Gong4Jinghao Chen5Xiangxiang Chen6Zihan Zhou7Liqun Zhang8Jian Xiao9Jiajia Xue10Beijing Laboratory of Biomedical Materials State Key Laboratory of Organic–Inorganic Composites Beijing University of Chemical Technology Beijing 100029 P. R. ChinaBeijing Laboratory of Biomedical Materials State Key Laboratory of Organic–Inorganic Composites Beijing University of Chemical Technology Beijing 100029 P. R. ChinaOujiang Laboratory School of Pharmaceutical Sciences Wenzhou Medical University Wenzhou 325035 ChinaBeijing Laboratory of Biomedical Materials State Key Laboratory of Organic–Inorganic Composites Beijing University of Chemical Technology Beijing 100029 P. R. ChinaBeijing Laboratory of Biomedical Materials State Key Laboratory of Organic–Inorganic Composites Beijing University of Chemical Technology Beijing 100029 P. R. ChinaOujiang Laboratory School of Pharmaceutical Sciences Wenzhou Medical University Wenzhou 325035 ChinaOujiang Laboratory School of Pharmaceutical Sciences Wenzhou Medical University Wenzhou 325035 ChinaOujiang Laboratory School of Pharmaceutical Sciences Wenzhou Medical University Wenzhou 325035 ChinaBeijing Laboratory of Biomedical Materials State Key Laboratory of Organic–Inorganic Composites Beijing University of Chemical Technology Beijing 100029 P. R. ChinaOujiang Laboratory School of Pharmaceutical Sciences Wenzhou Medical University Wenzhou 325035 ChinaBeijing Laboratory of Biomedical Materials State Key Laboratory of Organic–Inorganic Composites Beijing University of Chemical Technology Beijing 100029 P. R. ChinaAbstract Peripheral nerve injury is a serious medical problem with limited surgical and clinical treatment options. It is of great significance to integrate multiple guidance cues in one platform of nerve guidance conduits (NGCs) to promote axonal elongation and functional recovery. Here, a multi‐functional NGC is constructed to promote nerve regeneration by combining ordered topological structure, density gradient of biomacromolecular nanoparticles, and controlled delivery of biological effectors to provide the topographical, haptotactic, and biological cues, respectively. On the surface of aligned polycaprolactone nanofibers, a density gradient of bioactive nanoparticles capable of delivering recombinant human acidic fibroblast growth factor is deposited. On the graded scaffold, the proliferation of Schwann cells is promoted, and the directional extension of neurites from both PC12 cells and dorsal root ganglions is improved in the direction of increasing particle density. After being implanted in vivo for 6 and 12 weeks to repair a 10‐mm rat sciatic nerve defect, the NGC promotes axonal elongation and remyelination, achieving the regeneration of the nerve not only in anatomical structure but also in functional recovery. Taken together, the NGC provides a favorable microenvironment for peripheral nerve regeneration and holds great promise for realizing nerve repair with an efficacy close to autograft.https://doi.org/10.1002/advs.202203296controlled releaseelectrospun nanofibersgradient scaffoldsnerve guidance conduitsperipheral nerve repair |
| spellingShingle | Binghui Jin Yiling Yu Chenghao Lou Xiaodi Zhang Bowen Gong Jinghao Chen Xiangxiang Chen Zihan Zhou Liqun Zhang Jian Xiao Jiajia Xue Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber‐Based Nerve Guidance Conduit to Promote Peripheral Nerve Repair Advanced Science controlled release electrospun nanofibers gradient scaffolds nerve guidance conduits peripheral nerve repair |
| title | Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber‐Based Nerve Guidance Conduit to Promote Peripheral Nerve Repair |
| title_full | Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber‐Based Nerve Guidance Conduit to Promote Peripheral Nerve Repair |
| title_fullStr | Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber‐Based Nerve Guidance Conduit to Promote Peripheral Nerve Repair |
| title_full_unstemmed | Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber‐Based Nerve Guidance Conduit to Promote Peripheral Nerve Repair |
| title_short | Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber‐Based Nerve Guidance Conduit to Promote Peripheral Nerve Repair |
| title_sort | combining a density gradient of biomacromolecular nanoparticles with biological effectors in an electrospun fiber based nerve guidance conduit to promote peripheral nerve repair |
| topic | controlled release electrospun nanofibers gradient scaffolds nerve guidance conduits peripheral nerve repair |
| url | https://doi.org/10.1002/advs.202203296 |
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