In Situ Identification of Both IL-4 and IL-10 Cytokine–Receptor Interactions during Tissue Regeneration
Cytokines secreted by individual immune cells regulate tissue regeneration and allow communication between various cell types. Cytokines bind to cognate receptors and trigger the healing process. Determining the orchestration of cytokine interactions with their receptors on their cellular targets is...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/11/1522 |
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| author | Krisztina Nikovics Anne-Laure Favier Mathilde Rocher Céline Mayinga Johanna Gomez Frédérique Dufour-Gaume Diane Riccobono |
| author_facet | Krisztina Nikovics Anne-Laure Favier Mathilde Rocher Céline Mayinga Johanna Gomez Frédérique Dufour-Gaume Diane Riccobono |
| author_sort | Krisztina Nikovics |
| collection | DOAJ |
| description | Cytokines secreted by individual immune cells regulate tissue regeneration and allow communication between various cell types. Cytokines bind to cognate receptors and trigger the healing process. Determining the orchestration of cytokine interactions with their receptors on their cellular targets is essential to fully understanding the process of inflammation and tissue regeneration. To this end, we have investigated the interactions of Interleukin-4 cytokine (IL-4)/Interleukin-4 cytokine receptor (IL-4R) and Interleukin-10 cytokine (IL-10)/Interleukin-10 cytokine receptor (IL-10R) using in situ Proximity Ligation Assays in a regenerative model of skin, muscle and lung tissues in the mini-pig. The pattern of protein–protein interactions was distinct for the two cytokines. IL-4 bound predominantly to receptors on macrophages and endothelial cells around the blood vessels while the target cells of IL-10 were mainly receptors on muscle cells. Our results show that in situ studies of cytokine–receptor interactions can unravel the fine details of the mechanism of action of cytokines. |
| first_indexed | 2024-03-11T03:09:13Z |
| format | Article |
| id | doaj.art-0ca0cd784ff1420399be0f8fbff0a73b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T03:09:13Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-0ca0cd784ff1420399be0f8fbff0a73b2023-11-18T07:41:13ZengMDPI AGCells2073-44092023-05-011211152210.3390/cells12111522In Situ Identification of Both IL-4 and IL-10 Cytokine–Receptor Interactions during Tissue RegenerationKrisztina Nikovics0Anne-Laure Favier1Mathilde Rocher2Céline Mayinga3Johanna Gomez4Frédérique Dufour-Gaume5Diane Riccobono6Imagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223 Brétigny-sur-Orge, FranceImagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223 Brétigny-sur-Orge, FranceImagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223 Brétigny-sur-Orge, FranceImagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223 Brétigny-sur-Orge, FranceImagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223 Brétigny-sur-Orge, FranceWar Traumatology Unit, Department of NRBC Defense, French Armed Forces Biomedical Research Institute, 91223 Brétigny-sur-Orge, FranceDepartment of Radiation Bioeffects, French Armed Forces Biomedical Research Institute, 1, Place du Général Valérie André, 91223 Brétigny-sur-Orge, FranceCytokines secreted by individual immune cells regulate tissue regeneration and allow communication between various cell types. Cytokines bind to cognate receptors and trigger the healing process. Determining the orchestration of cytokine interactions with their receptors on their cellular targets is essential to fully understanding the process of inflammation and tissue regeneration. To this end, we have investigated the interactions of Interleukin-4 cytokine (IL-4)/Interleukin-4 cytokine receptor (IL-4R) and Interleukin-10 cytokine (IL-10)/Interleukin-10 cytokine receptor (IL-10R) using in situ Proximity Ligation Assays in a regenerative model of skin, muscle and lung tissues in the mini-pig. The pattern of protein–protein interactions was distinct for the two cytokines. IL-4 bound predominantly to receptors on macrophages and endothelial cells around the blood vessels while the target cells of IL-10 were mainly receptors on muscle cells. Our results show that in situ studies of cytokine–receptor interactions can unravel the fine details of the mechanism of action of cytokines.https://www.mdpi.com/2073-4409/12/11/1522IL-4IL-10receptorinteractionregenerationproximity ligation assay |
| spellingShingle | Krisztina Nikovics Anne-Laure Favier Mathilde Rocher Céline Mayinga Johanna Gomez Frédérique Dufour-Gaume Diane Riccobono In Situ Identification of Both IL-4 and IL-10 Cytokine–Receptor Interactions during Tissue Regeneration Cells IL-4 IL-10 receptor interaction regeneration proximity ligation assay |
| title | In Situ Identification of Both IL-4 and IL-10 Cytokine–Receptor Interactions during Tissue Regeneration |
| title_full | In Situ Identification of Both IL-4 and IL-10 Cytokine–Receptor Interactions during Tissue Regeneration |
| title_fullStr | In Situ Identification of Both IL-4 and IL-10 Cytokine–Receptor Interactions during Tissue Regeneration |
| title_full_unstemmed | In Situ Identification of Both IL-4 and IL-10 Cytokine–Receptor Interactions during Tissue Regeneration |
| title_short | In Situ Identification of Both IL-4 and IL-10 Cytokine–Receptor Interactions during Tissue Regeneration |
| title_sort | in situ identification of both il 4 and il 10 cytokine receptor interactions during tissue regeneration |
| topic | IL-4 IL-10 receptor interaction regeneration proximity ligation assay |
| url | https://www.mdpi.com/2073-4409/12/11/1522 |
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