Chemical Composition, Antioxidant, and Antitumor Activity of Fucoidan from the Brown Alga <i>Dictyota dichotoma</i>
Brown macroalgae are a rich source of fucoidans with many pharmacological uses. This research aimed to isolate and characterize fucoidan from <i>Dictyota dichotoma</i> var. <i>dichotoma</i> (Hudson) J.V. Lamouroux and evaluate <i>in vitro</i> its antioxidant and a...
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MDPI AG
2023-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/20/7175 |
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| author | Mostafa M. El-Sheekh Fatma Ward Mohamed A. Deyab Majid Al-Zahrani Hussein E. Touliabah |
| author_facet | Mostafa M. El-Sheekh Fatma Ward Mohamed A. Deyab Majid Al-Zahrani Hussein E. Touliabah |
| author_sort | Mostafa M. El-Sheekh |
| collection | DOAJ |
| description | Brown macroalgae are a rich source of fucoidans with many pharmacological uses. This research aimed to isolate and characterize fucoidan from <i>Dictyota dichotoma</i> var. <i>dichotoma</i> (Hudson) J.V. Lamouroux and evaluate <i>in vitro</i> its antioxidant and antitumor potential. The fucoidan yield was 0.057 g/g algal dry wt with a molecular weight of about 48.6 kDa. In terms of fucoidan composition, the sulfate, uronic acid, and protein contents were 83.3 ± 5.20 mg/g fucoidan, 22.5 ± 0.80 mg/g fucoidan, and 26.1 ± 1.70 mg/g fucoidan, respectively. Fucose was the primary sugar component, as were glucose, galactose, mannose, xylose, and glucuronic acid. Fucoidan exhibited strong antioxidant potential that increased by more than 3 times with the increase in concentration from 0.1 to 5.0 mg/mL. Moreover, different concentrations of fucoidan (0.05–1 mg/mL) showed their ability to decrease the viability of Ehrlich ascites carcinoma cells in a time-dependent manner. These findings provided a fast method to obtain an appreciable amount of natural fucoidan with established structural characteristics as a promising compound with pronounced antioxidant and anticancer activity. |
| first_indexed | 2024-03-10T21:00:15Z |
| format | Article |
| id | doaj.art-0ca418f29fca49acbda53fd4d61507a1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T21:00:15Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0ca418f29fca49acbda53fd4d61507a12023-11-19T17:33:52ZengMDPI AGMolecules1420-30492023-10-012820717510.3390/molecules28207175Chemical Composition, Antioxidant, and Antitumor Activity of Fucoidan from the Brown Alga <i>Dictyota dichotoma</i>Mostafa M. El-Sheekh0Fatma Ward1Mohamed A. Deyab2Majid Al-Zahrani3Hussein E. Touliabah4Department of Botany, Faculty of Science, Tanta University, Tanta 31527, EgyptDepartment of Botany and Microbiology, Faculty of Science, Damietta University, New Damietta City 34511, EgyptDepartment of Botany and Microbiology, Faculty of Science, Damietta University, New Damietta City 34511, EgyptDepartment of Biological Science, College of Science and Arts at Rabigh, King Abdulaziz University, Rabigh 25732, Saudi ArabiaFaculty of Women for Ats, Science and Education, Ain Shams University, Cairo 11757, EgyptBrown macroalgae are a rich source of fucoidans with many pharmacological uses. This research aimed to isolate and characterize fucoidan from <i>Dictyota dichotoma</i> var. <i>dichotoma</i> (Hudson) J.V. Lamouroux and evaluate <i>in vitro</i> its antioxidant and antitumor potential. The fucoidan yield was 0.057 g/g algal dry wt with a molecular weight of about 48.6 kDa. In terms of fucoidan composition, the sulfate, uronic acid, and protein contents were 83.3 ± 5.20 mg/g fucoidan, 22.5 ± 0.80 mg/g fucoidan, and 26.1 ± 1.70 mg/g fucoidan, respectively. Fucose was the primary sugar component, as were glucose, galactose, mannose, xylose, and glucuronic acid. Fucoidan exhibited strong antioxidant potential that increased by more than 3 times with the increase in concentration from 0.1 to 5.0 mg/mL. Moreover, different concentrations of fucoidan (0.05–1 mg/mL) showed their ability to decrease the viability of Ehrlich ascites carcinoma cells in a time-dependent manner. These findings provided a fast method to obtain an appreciable amount of natural fucoidan with established structural characteristics as a promising compound with pronounced antioxidant and anticancer activity.https://www.mdpi.com/1420-3049/28/20/7175<i>Dictyota dichotoma</i>antioxidant activityantitumor activityFucoidan with sulphated polysaccharidesPhaeophytaRed Sea |
| spellingShingle | Mostafa M. El-Sheekh Fatma Ward Mohamed A. Deyab Majid Al-Zahrani Hussein E. Touliabah Chemical Composition, Antioxidant, and Antitumor Activity of Fucoidan from the Brown Alga <i>Dictyota dichotoma</i> Molecules <i>Dictyota dichotoma</i> antioxidant activity antitumor activity Fucoidan with sulphated polysaccharides Phaeophyta Red Sea |
| title | Chemical Composition, Antioxidant, and Antitumor Activity of Fucoidan from the Brown Alga <i>Dictyota dichotoma</i> |
| title_full | Chemical Composition, Antioxidant, and Antitumor Activity of Fucoidan from the Brown Alga <i>Dictyota dichotoma</i> |
| title_fullStr | Chemical Composition, Antioxidant, and Antitumor Activity of Fucoidan from the Brown Alga <i>Dictyota dichotoma</i> |
| title_full_unstemmed | Chemical Composition, Antioxidant, and Antitumor Activity of Fucoidan from the Brown Alga <i>Dictyota dichotoma</i> |
| title_short | Chemical Composition, Antioxidant, and Antitumor Activity of Fucoidan from the Brown Alga <i>Dictyota dichotoma</i> |
| title_sort | chemical composition antioxidant and antitumor activity of fucoidan from the brown alga i dictyota dichotoma i |
| topic | <i>Dictyota dichotoma</i> antioxidant activity antitumor activity Fucoidan with sulphated polysaccharides Phaeophyta Red Sea |
| url | https://www.mdpi.com/1420-3049/28/20/7175 |
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