Sonochemical synthesis of protein microcapsules loaded with traditional Chinese herb extracts
Traditional Chinese herbs have attracted extensive attention due to their good efficacy, low toxicity, and minor side effects. However, some active ingredient extracts are relatively sensitive to external influence and liable to lose their effectiveness. Different from the traditional and complex en...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2022-02-01
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| Series: | Green Processing and Synthesis |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/gps-2022-0013 |
| _version_ | 1811236608963248128 |
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| author | Wang Xin Wang Zhe Guo Shengwei Wang Guxia Xue Min |
| author_facet | Wang Xin Wang Zhe Guo Shengwei Wang Guxia Xue Min |
| author_sort | Wang Xin |
| collection | DOAJ |
| description | Traditional Chinese herbs have attracted extensive attention due to their good efficacy, low toxicity, and minor side effects. However, some active ingredient extracts are relatively sensitive to external influence and liable to lose their effectiveness. Different from the traditional and complex encapsulation methods, one can easily prepare drug-loaded microcapsules to improve their bioavailability using the new encapsulation technology. In this work, we used the sonochemical method to prepare bovine serum albumin (BSA)/astragalus membranaceus oil (AM) microcapsules. The technology was simpler, greener, and more efficient. Thereinto, BSA and AM oil were used as the shell material and the core material, respectively. The effects of ultrasonic amplitude, ultrasonic mode, and ultrasonic time on synthetic reactions were studied. The morphology and size of the BSA/AM microcapsules were investigated using transmission electron microscopy, scanning electron microscopy, and dynamic light scattering (DLS). The loading efficiency and drug release behavior were determined using thermogravimetric analysis (TGA) and ultraviolet (UV) spectroscopy, respectively. The best ultrasonic synthesis conditions were obtained by the above analysis, with an ultrasound amplitude of 30%, ultrasound mode of the pulse mode of 2 s, ultrasound time of 4 min. The DLS results show that the microcapsule size is 484.4 nm and the polydispersity index is small. The TGA results show that the drug loading efficiency is about 77%, and the hemolysis tests show that the BSA/AM microcapsules have no cytotoxicity at lower concentrations (lower than 50 μg·mL−1). |
| first_indexed | 2024-04-12T12:10:53Z |
| format | Article |
| id | doaj.art-0ca6fc4e554c4474b36cf2ec0fdf14d5 |
| institution | Directory Open Access Journal |
| issn | 2191-9550 |
| language | English |
| last_indexed | 2024-04-12T12:10:53Z |
| publishDate | 2022-02-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Green Processing and Synthesis |
| spelling | doaj.art-0ca6fc4e554c4474b36cf2ec0fdf14d52022-12-22T03:33:34ZengDe GruyterGreen Processing and Synthesis2191-95502022-02-0111116417510.1515/gps-2022-0013Sonochemical synthesis of protein microcapsules loaded with traditional Chinese herb extractsWang Xin0Wang Zhe1Guo Shengwei2Wang Guxia3Xue Min4Department of Polymer Materials and Engineering, School of Materials Science and Engineering, North Minzu University, Yinchuan 750021, ChinaDepartment of Polymer Materials and Engineering, School of Materials Science and Engineering, North Minzu University, Yinchuan 750021, ChinaDepartment of Polymer Materials and Engineering, School of Materials Science and Engineering, North Minzu University, Yinchuan 750021, ChinaDepartment of Chemical Engineering and Technology, School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, ChinaDepartment of Polymer Materials and Engineering, School of Materials Science and Engineering, North Minzu University, Yinchuan 750021, ChinaTraditional Chinese herbs have attracted extensive attention due to their good efficacy, low toxicity, and minor side effects. However, some active ingredient extracts are relatively sensitive to external influence and liable to lose their effectiveness. Different from the traditional and complex encapsulation methods, one can easily prepare drug-loaded microcapsules to improve their bioavailability using the new encapsulation technology. In this work, we used the sonochemical method to prepare bovine serum albumin (BSA)/astragalus membranaceus oil (AM) microcapsules. The technology was simpler, greener, and more efficient. Thereinto, BSA and AM oil were used as the shell material and the core material, respectively. The effects of ultrasonic amplitude, ultrasonic mode, and ultrasonic time on synthetic reactions were studied. The morphology and size of the BSA/AM microcapsules were investigated using transmission electron microscopy, scanning electron microscopy, and dynamic light scattering (DLS). The loading efficiency and drug release behavior were determined using thermogravimetric analysis (TGA) and ultraviolet (UV) spectroscopy, respectively. The best ultrasonic synthesis conditions were obtained by the above analysis, with an ultrasound amplitude of 30%, ultrasound mode of the pulse mode of 2 s, ultrasound time of 4 min. The DLS results show that the microcapsule size is 484.4 nm and the polydispersity index is small. The TGA results show that the drug loading efficiency is about 77%, and the hemolysis tests show that the BSA/AM microcapsules have no cytotoxicity at lower concentrations (lower than 50 μg·mL−1).https://doi.org/10.1515/gps-2022-0013sonochemistrychinese herb microcapsulesbovine serum albuminastragalus membranaceus oil |
| spellingShingle | Wang Xin Wang Zhe Guo Shengwei Wang Guxia Xue Min Sonochemical synthesis of protein microcapsules loaded with traditional Chinese herb extracts Green Processing and Synthesis sonochemistry chinese herb microcapsules bovine serum albumin astragalus membranaceus oil |
| title | Sonochemical synthesis of protein microcapsules loaded with traditional Chinese herb extracts |
| title_full | Sonochemical synthesis of protein microcapsules loaded with traditional Chinese herb extracts |
| title_fullStr | Sonochemical synthesis of protein microcapsules loaded with traditional Chinese herb extracts |
| title_full_unstemmed | Sonochemical synthesis of protein microcapsules loaded with traditional Chinese herb extracts |
| title_short | Sonochemical synthesis of protein microcapsules loaded with traditional Chinese herb extracts |
| title_sort | sonochemical synthesis of protein microcapsules loaded with traditional chinese herb extracts |
| topic | sonochemistry chinese herb microcapsules bovine serum albumin astragalus membranaceus oil |
| url | https://doi.org/10.1515/gps-2022-0013 |
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