Enhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. AK13
Abstract Microbially induced calcium carbonate (CaCO3) precipitation (MICP) is a process where microbes induce condition favorable for CaCO3 formation through metabolic activities by increasing the pH or carbonate ions when calcium is near. The molecular and ecological basis of CaCO3 precipitating (...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-04-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13568-019-0773-x |
| _version_ | 1818353190209323008 |
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| author | Yun Suk Lee Woojun Park |
| author_facet | Yun Suk Lee Woojun Park |
| author_sort | Yun Suk Lee |
| collection | DOAJ |
| description | Abstract Microbially induced calcium carbonate (CaCO3) precipitation (MICP) is a process where microbes induce condition favorable for CaCO3 formation through metabolic activities by increasing the pH or carbonate ions when calcium is near. The molecular and ecological basis of CaCO3 precipitating (CCP) bacteria has been poorly illuminated. Here, we showed that increased pH levels by deamination of amino acids is a driving force toward MICP using alkalitolerant Lysinibacillus boronitolerans YS11 as a model species of non-ureolytic CCP bacteria. This alkaline generation also facilitates the growth of neighboring alkaliphilic Bacillus sp. AK13, which could alter characteristics of MICP by changing the size and shape of CaCO3 minerals. Furthermore, we showed CaCO3 that precipitates earlier in an experiment modifies membrane rigidity of YS11 strain via upregulation of branched chain fatty acid synthesis. This work closely examines MICP conditions by deamination and the effect of MICP on cell membrane rigidity and crystal formation for the first time. |
| first_indexed | 2024-12-13T19:05:35Z |
| format | Article |
| id | doaj.art-0caa466b585140af946990c6e567f903 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-13T19:05:35Z |
| publishDate | 2019-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-0caa466b585140af946990c6e567f9032022-12-21T23:34:33ZengSpringerOpenAMB Express2191-08552019-04-019111710.1186/s13568-019-0773-xEnhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. AK13Yun Suk Lee0Woojun Park1Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea UniversityLaboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea UniversityAbstract Microbially induced calcium carbonate (CaCO3) precipitation (MICP) is a process where microbes induce condition favorable for CaCO3 formation through metabolic activities by increasing the pH or carbonate ions when calcium is near. The molecular and ecological basis of CaCO3 precipitating (CCP) bacteria has been poorly illuminated. Here, we showed that increased pH levels by deamination of amino acids is a driving force toward MICP using alkalitolerant Lysinibacillus boronitolerans YS11 as a model species of non-ureolytic CCP bacteria. This alkaline generation also facilitates the growth of neighboring alkaliphilic Bacillus sp. AK13, which could alter characteristics of MICP by changing the size and shape of CaCO3 minerals. Furthermore, we showed CaCO3 that precipitates earlier in an experiment modifies membrane rigidity of YS11 strain via upregulation of branched chain fatty acid synthesis. This work closely examines MICP conditions by deamination and the effect of MICP on cell membrane rigidity and crystal formation for the first time.http://link.springer.com/article/10.1186/s13568-019-0773-xAlkaline generationDual species CaCO3 precipitationBacteria-CaCO3 interactionBranched chain fatty acid synthesisMembrane rigidity |
| spellingShingle | Yun Suk Lee Woojun Park Enhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. AK13 AMB Express Alkaline generation Dual species CaCO3 precipitation Bacteria-CaCO3 interaction Branched chain fatty acid synthesis Membrane rigidity |
| title | Enhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. AK13 |
| title_full | Enhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. AK13 |
| title_fullStr | Enhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. AK13 |
| title_full_unstemmed | Enhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. AK13 |
| title_short | Enhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. AK13 |
| title_sort | enhanced calcium carbonate biofilm complex formation by alkali generating lysinibacillus boronitolerans ys11 and alkaliphilic bacillus sp ak13 |
| topic | Alkaline generation Dual species CaCO3 precipitation Bacteria-CaCO3 interaction Branched chain fatty acid synthesis Membrane rigidity |
| url | http://link.springer.com/article/10.1186/s13568-019-0773-x |
| work_keys_str_mv | AT yunsuklee enhancedcalciumcarbonatebiofilmcomplexformationbyalkaligeneratinglysinibacillusboronitoleransys11andalkaliphilicbacillusspak13 AT woojunpark enhancedcalciumcarbonatebiofilmcomplexformationbyalkaligeneratinglysinibacillusboronitoleransys11andalkaliphilicbacillusspak13 |