First isolation and genomic characterization of avian reovirus from black swans (Cygnus atratus) in China
ABSTRACT: Identification and analysis of the avian reovirus from black swan.Isolation of the strain through the chorioallantoic membrane route of duck embryos, identified through transmission electron microscopy and RT-PCR based on the ARV S2 gene. The complete genome of the ARV strain was obtained...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123004662 |
| _version_ | 1827808388436721664 |
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| author | Dekang Zhu Rong Sun Mingshu Wang Renyong Jia Shun Chen Mafeng Liu Xinxin Zhao Qiao Yang Ying Wu Shaqiu Zhang Juan Huang Xumin Ou Sai Mao Qun Gao Di Sun Bin Tian Anchun Cheng |
| author_facet | Dekang Zhu Rong Sun Mingshu Wang Renyong Jia Shun Chen Mafeng Liu Xinxin Zhao Qiao Yang Ying Wu Shaqiu Zhang Juan Huang Xumin Ou Sai Mao Qun Gao Di Sun Bin Tian Anchun Cheng |
| author_sort | Dekang Zhu |
| collection | DOAJ |
| description | ABSTRACT: Identification and analysis of the avian reovirus from black swan.Isolation of the strain through the chorioallantoic membrane route of duck embryos, identified through transmission electron microscopy and RT-PCR based on the ARV S2 gene. The complete genome of the ARV strain was obtained using next-generation sequencing technology.The isolated strain of ARV was named CD200801 and was identified through transmission electron microscopy and RT-PCR based on the ARV S2 gene. Experimental infection with CD200801 resulted in the death of ducklings with serious spleen and liver focal necrosis. BLAST analysis of CD200801 sequences showed a 35.5 to 98.6% similarity to a novel duck reovirus that was isolated in recent years. Phylogenetic analysis revealed that CD200801 was closely related to ARV isolates YL, GX-Y7, and XT-18.We report the first avian reovirus infection in the black swan. This study provides important new insights into the evolutionary relationships among different ARV strains and highlights the need for continued surveillance and monitoring of these viruses in both domestic and wild bird flocks. These findings have significant implications for the development of effective strategies for disease prevention and control in the poultry industry. |
| first_indexed | 2024-03-11T22:26:29Z |
| format | Article |
| id | doaj.art-0caaf2d05b5945e585ee1e85457325e6 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-11T22:26:29Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-0caaf2d05b5945e585ee1e85457325e62023-09-24T05:13:56ZengElsevierPoultry Science0032-57912023-10-0110210102947First isolation and genomic characterization of avian reovirus from black swans (Cygnus atratus) in ChinaDekang Zhu0Rong Sun1Mingshu Wang2Renyong Jia3Shun Chen4Mafeng Liu5Xinxin Zhao6Qiao Yang7Ying Wu8Shaqiu Zhang9Juan Huang10Xumin Ou11Sai Mao12Qun Gao13Di Sun14Bin Tian15Anchun Cheng16Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, China; Corresponding author:ABSTRACT: Identification and analysis of the avian reovirus from black swan.Isolation of the strain through the chorioallantoic membrane route of duck embryos, identified through transmission electron microscopy and RT-PCR based on the ARV S2 gene. The complete genome of the ARV strain was obtained using next-generation sequencing technology.The isolated strain of ARV was named CD200801 and was identified through transmission electron microscopy and RT-PCR based on the ARV S2 gene. Experimental infection with CD200801 resulted in the death of ducklings with serious spleen and liver focal necrosis. BLAST analysis of CD200801 sequences showed a 35.5 to 98.6% similarity to a novel duck reovirus that was isolated in recent years. Phylogenetic analysis revealed that CD200801 was closely related to ARV isolates YL, GX-Y7, and XT-18.We report the first avian reovirus infection in the black swan. This study provides important new insights into the evolutionary relationships among different ARV strains and highlights the need for continued surveillance and monitoring of these viruses in both domestic and wild bird flocks. These findings have significant implications for the development of effective strategies for disease prevention and control in the poultry industry.http://www.sciencedirect.com/science/article/pii/S0032579123004662avian reovirusblack swansgenomic characterization |
| spellingShingle | Dekang Zhu Rong Sun Mingshu Wang Renyong Jia Shun Chen Mafeng Liu Xinxin Zhao Qiao Yang Ying Wu Shaqiu Zhang Juan Huang Xumin Ou Sai Mao Qun Gao Di Sun Bin Tian Anchun Cheng First isolation and genomic characterization of avian reovirus from black swans (Cygnus atratus) in China Poultry Science avian reovirus black swans genomic characterization |
| title | First isolation and genomic characterization of avian reovirus from black swans (Cygnus atratus) in China |
| title_full | First isolation and genomic characterization of avian reovirus from black swans (Cygnus atratus) in China |
| title_fullStr | First isolation and genomic characterization of avian reovirus from black swans (Cygnus atratus) in China |
| title_full_unstemmed | First isolation and genomic characterization of avian reovirus from black swans (Cygnus atratus) in China |
| title_short | First isolation and genomic characterization of avian reovirus from black swans (Cygnus atratus) in China |
| title_sort | first isolation and genomic characterization of avian reovirus from black swans cygnus atratus in china |
| topic | avian reovirus black swans genomic characterization |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123004662 |
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