The HER2-binding affibody molecule (Z(HER2∶342))₂ increases radiosensitivity in SKBR-3 cells.
We have previously shown that the HER2-specific affibody molecule (Z(HER2∶342))₂ inhibits proliferation of SKBR-3 cells. Here, we continue to investigate its biological effects in vitro by studying receptor dimerization and clonogenic survival following irradiation. We found that (Z(HER2∶342))₂ sens...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3498194?pdf=render |
| _version_ | 1828239400443576320 |
|---|---|
| author | Lina Ekerljung Johan Lennartsson Lars Gedda |
| author_facet | Lina Ekerljung Johan Lennartsson Lars Gedda |
| author_sort | Lina Ekerljung |
| collection | DOAJ |
| description | We have previously shown that the HER2-specific affibody molecule (Z(HER2∶342))₂ inhibits proliferation of SKBR-3 cells. Here, we continue to investigate its biological effects in vitro by studying receptor dimerization and clonogenic survival following irradiation. We found that (Z(HER2∶342))₂ sensitizes the HER2-overexpressing cell line SKBR-3 to ionizing radiation. The survival after exposure to (Z(HER2∶342))₂ and 8 Gy (S(8Gy) 0.006) was decreased by a factor four compared to the untreated (S(8Gy) 0.023). The low HER2-expressing cell line MCF-7 was more radiosensitive than SKBR-3 but did not respond to (Z(HER2∶342))₂. Treatment by (Z(HER2∶342))₂ strongly increased the levels of dimerized and phosphorylated HER2 even after 5 minutes of stimulation. The monomeric Z(HER2∶342) does not seem to be able to induce receptor phosphorylation and dimerization or sensitize cells to irradiation. |
| first_indexed | 2024-04-12T21:23:11Z |
| format | Article |
| id | doaj.art-0cad62d0c83d47ae9a3e7bd609e90c24 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T21:23:11Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0cad62d0c83d47ae9a3e7bd609e90c242022-12-22T03:16:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01711e4957910.1371/journal.pone.0049579The HER2-binding affibody molecule (Z(HER2∶342))₂ increases radiosensitivity in SKBR-3 cells.Lina EkerljungJohan LennartssonLars GeddaWe have previously shown that the HER2-specific affibody molecule (Z(HER2∶342))₂ inhibits proliferation of SKBR-3 cells. Here, we continue to investigate its biological effects in vitro by studying receptor dimerization and clonogenic survival following irradiation. We found that (Z(HER2∶342))₂ sensitizes the HER2-overexpressing cell line SKBR-3 to ionizing radiation. The survival after exposure to (Z(HER2∶342))₂ and 8 Gy (S(8Gy) 0.006) was decreased by a factor four compared to the untreated (S(8Gy) 0.023). The low HER2-expressing cell line MCF-7 was more radiosensitive than SKBR-3 but did not respond to (Z(HER2∶342))₂. Treatment by (Z(HER2∶342))₂ strongly increased the levels of dimerized and phosphorylated HER2 even after 5 minutes of stimulation. The monomeric Z(HER2∶342) does not seem to be able to induce receptor phosphorylation and dimerization or sensitize cells to irradiation.http://europepmc.org/articles/PMC3498194?pdf=render |
| spellingShingle | Lina Ekerljung Johan Lennartsson Lars Gedda The HER2-binding affibody molecule (Z(HER2∶342))₂ increases radiosensitivity in SKBR-3 cells. PLoS ONE |
| title | The HER2-binding affibody molecule (Z(HER2∶342))₂ increases radiosensitivity in SKBR-3 cells. |
| title_full | The HER2-binding affibody molecule (Z(HER2∶342))₂ increases radiosensitivity in SKBR-3 cells. |
| title_fullStr | The HER2-binding affibody molecule (Z(HER2∶342))₂ increases radiosensitivity in SKBR-3 cells. |
| title_full_unstemmed | The HER2-binding affibody molecule (Z(HER2∶342))₂ increases radiosensitivity in SKBR-3 cells. |
| title_short | The HER2-binding affibody molecule (Z(HER2∶342))₂ increases radiosensitivity in SKBR-3 cells. |
| title_sort | her2 binding affibody molecule z her2∶342 ₂ increases radiosensitivity in skbr 3 cells |
| url | http://europepmc.org/articles/PMC3498194?pdf=render |
| work_keys_str_mv | AT linaekerljung theher2bindingaffibodymoleculezher23422increasesradiosensitivityinskbr3cells AT johanlennartsson theher2bindingaffibodymoleculezher23422increasesradiosensitivityinskbr3cells AT larsgedda theher2bindingaffibodymoleculezher23422increasesradiosensitivityinskbr3cells AT linaekerljung her2bindingaffibodymoleculezher23422increasesradiosensitivityinskbr3cells AT johanlennartsson her2bindingaffibodymoleculezher23422increasesradiosensitivityinskbr3cells AT larsgedda her2bindingaffibodymoleculezher23422increasesradiosensitivityinskbr3cells |