Development of Antimicrobial Laser-Induced Photodynamic Therapy Based on Ethylcellulose/Chitosan Nanocomposite with 5,10,15,20-Tetrakis(<i>m</i>-Hydroxyphenyl)porphyrin
The development of new antimicrobial strategies that act more efficiently than traditional antibiotics is becoming a necessity to combat multidrug-resistant pathogens. Here we report the efficacy of laser-light-irradiated 5,10,15,20-tetrakis(<i>m</i>-hydroxyphenyl)porphyrin (<i>m&l...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-06-01
|
Series: | Molecules |
Subjects: | |
Online Access: | https://www.mdpi.com/1420-3049/26/12/3551 |
_version_ | 1797530570214866944 |
---|---|
author | Mohamed S. Hasanin Mohamed Abdelraof Mohamed Fikry Yasser M. Shaker Ayman M. K. Sweed Mathias O. Senge |
author_facet | Mohamed S. Hasanin Mohamed Abdelraof Mohamed Fikry Yasser M. Shaker Ayman M. K. Sweed Mathias O. Senge |
author_sort | Mohamed S. Hasanin |
collection | DOAJ |
description | The development of new antimicrobial strategies that act more efficiently than traditional antibiotics is becoming a necessity to combat multidrug-resistant pathogens. Here we report the efficacy of laser-light-irradiated 5,10,15,20-tetrakis(<i>m</i>-hydroxyphenyl)porphyrin (<i>m</i>THPP) loaded onto an ethylcellulose (EC)/chitosan (Chs) nanocomposite in eradicating multi-drug resistant <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i>, and <i>Candida albicans.</i> Surface loading of the ethylcelllose/chitosan composite with <i>m</i>THPP was carried out and the resulting nanocomposite was fully characterized. The results indicate that the prepared nanocomposite incorporates <i>m</i>THPP inside, and that the composite acquired an overall positive charge. The incorporation of <i>m</i>THPP into the nanocomposite enhanced the photo- and thermal stability. Different laser wavelengths (458; 476; 488; 515; 635 nm), powers (5–70 mW), and exposure times (15–45 min) were investigated in the antimicrobial photodynamic therapy (aPDT) experiments, with the best inhibition observed using 635 nm with the <i>m</i>THPP EC/Chs nanocomposite for <i>C. albicans</i> (59 ± 0.21%), <i>P. aeruginosa</i> (71.7 ± 1.72%), and <i>S. aureus</i> (74.2 ± 1.26%) with illumination of only 15 min. Utilization of higher doses (70 mW) for longer periods achieved more eradication of microbial growth. |
first_indexed | 2024-03-10T10:31:52Z |
format | Article |
id | doaj.art-0cb05dd91ad142758fb0b325246d1c27 |
institution | Directory Open Access Journal |
issn | 1420-3049 |
language | English |
last_indexed | 2024-03-10T10:31:52Z |
publishDate | 2021-06-01 |
publisher | MDPI AG |
record_format | Article |
series | Molecules |
spelling | doaj.art-0cb05dd91ad142758fb0b325246d1c272023-11-21T23:35:11ZengMDPI AGMolecules1420-30492021-06-012612355110.3390/molecules26123551Development of Antimicrobial Laser-Induced Photodynamic Therapy Based on Ethylcellulose/Chitosan Nanocomposite with 5,10,15,20-Tetrakis(<i>m</i>-Hydroxyphenyl)porphyrinMohamed S. Hasanin0Mohamed Abdelraof1Mohamed Fikry2Yasser M. Shaker3Ayman M. K. Sweed4Mathias O. Senge5Cellulose & Paper Department, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Giza P.O. 12622, EgyptMicrobial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Giza P.O. 12622, EgyptPhysics Department, Faculty of Science, Cairo University, Giza P.O. 12613, EgyptChemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Division, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Giza P.O. 12622, EgyptChemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Division, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Giza P.O. 12622, EgyptMedicinal Chemistry, Trinity Translational Medicine Institute, Trinity Centre for Health Sciences, Trinity College Dublin, The University of Dublin, St. James’s Hospital, Dublin 8, IrelandThe development of new antimicrobial strategies that act more efficiently than traditional antibiotics is becoming a necessity to combat multidrug-resistant pathogens. Here we report the efficacy of laser-light-irradiated 5,10,15,20-tetrakis(<i>m</i>-hydroxyphenyl)porphyrin (<i>m</i>THPP) loaded onto an ethylcellulose (EC)/chitosan (Chs) nanocomposite in eradicating multi-drug resistant <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i>, and <i>Candida albicans.</i> Surface loading of the ethylcelllose/chitosan composite with <i>m</i>THPP was carried out and the resulting nanocomposite was fully characterized. The results indicate that the prepared nanocomposite incorporates <i>m</i>THPP inside, and that the composite acquired an overall positive charge. The incorporation of <i>m</i>THPP into the nanocomposite enhanced the photo- and thermal stability. Different laser wavelengths (458; 476; 488; 515; 635 nm), powers (5–70 mW), and exposure times (15–45 min) were investigated in the antimicrobial photodynamic therapy (aPDT) experiments, with the best inhibition observed using 635 nm with the <i>m</i>THPP EC/Chs nanocomposite for <i>C. albicans</i> (59 ± 0.21%), <i>P. aeruginosa</i> (71.7 ± 1.72%), and <i>S. aureus</i> (74.2 ± 1.26%) with illumination of only 15 min. Utilization of higher doses (70 mW) for longer periods achieved more eradication of microbial growth.https://www.mdpi.com/1420-3049/26/12/3551<i>m</i>THPPethylcellulosechitosannanocompositemultidrug resistancephotodynamic therapy |
spellingShingle | Mohamed S. Hasanin Mohamed Abdelraof Mohamed Fikry Yasser M. Shaker Ayman M. K. Sweed Mathias O. Senge Development of Antimicrobial Laser-Induced Photodynamic Therapy Based on Ethylcellulose/Chitosan Nanocomposite with 5,10,15,20-Tetrakis(<i>m</i>-Hydroxyphenyl)porphyrin Molecules <i>m</i>THPP ethylcellulose chitosan nanocomposite multidrug resistance photodynamic therapy |
title | Development of Antimicrobial Laser-Induced Photodynamic Therapy Based on Ethylcellulose/Chitosan Nanocomposite with 5,10,15,20-Tetrakis(<i>m</i>-Hydroxyphenyl)porphyrin |
title_full | Development of Antimicrobial Laser-Induced Photodynamic Therapy Based on Ethylcellulose/Chitosan Nanocomposite with 5,10,15,20-Tetrakis(<i>m</i>-Hydroxyphenyl)porphyrin |
title_fullStr | Development of Antimicrobial Laser-Induced Photodynamic Therapy Based on Ethylcellulose/Chitosan Nanocomposite with 5,10,15,20-Tetrakis(<i>m</i>-Hydroxyphenyl)porphyrin |
title_full_unstemmed | Development of Antimicrobial Laser-Induced Photodynamic Therapy Based on Ethylcellulose/Chitosan Nanocomposite with 5,10,15,20-Tetrakis(<i>m</i>-Hydroxyphenyl)porphyrin |
title_short | Development of Antimicrobial Laser-Induced Photodynamic Therapy Based on Ethylcellulose/Chitosan Nanocomposite with 5,10,15,20-Tetrakis(<i>m</i>-Hydroxyphenyl)porphyrin |
title_sort | development of antimicrobial laser induced photodynamic therapy based on ethylcellulose chitosan nanocomposite with 5 10 15 20 tetrakis i m i hydroxyphenyl porphyrin |
topic | <i>m</i>THPP ethylcellulose chitosan nanocomposite multidrug resistance photodynamic therapy |
url | https://www.mdpi.com/1420-3049/26/12/3551 |
work_keys_str_mv | AT mohamedshasanin developmentofantimicrobiallaserinducedphotodynamictherapybasedonethylcellulosechitosannanocompositewith5101520tetrakisimihydroxyphenylporphyrin AT mohamedabdelraof developmentofantimicrobiallaserinducedphotodynamictherapybasedonethylcellulosechitosannanocompositewith5101520tetrakisimihydroxyphenylporphyrin AT mohamedfikry developmentofantimicrobiallaserinducedphotodynamictherapybasedonethylcellulosechitosannanocompositewith5101520tetrakisimihydroxyphenylporphyrin AT yassermshaker developmentofantimicrobiallaserinducedphotodynamictherapybasedonethylcellulosechitosannanocompositewith5101520tetrakisimihydroxyphenylporphyrin AT aymanmksweed developmentofantimicrobiallaserinducedphotodynamictherapybasedonethylcellulosechitosannanocompositewith5101520tetrakisimihydroxyphenylporphyrin AT mathiasosenge developmentofantimicrobiallaserinducedphotodynamictherapybasedonethylcellulosechitosannanocompositewith5101520tetrakisimihydroxyphenylporphyrin |