Genome comparison of the epiphytic bacteria <it>Erwinia billingiae </it>and <it>E. tasmaniensis </it>with the pear pathogen <it>E. pyrifoliae</it>
<p>Abstract</p> <p>Background</p> <p>The genus <it>Erwinia </it>includes plant-associated pathogenic and non-pathogenic <it>Enterobacteria</it>. Important pathogens such as <it>Erwinia amylovora</it>, the causative agent of fire bligh...
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| Format: | Article |
| Language: | English |
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BMC
2010-06-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/11/393 |
| _version_ | 1811320744219508736 |
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| author | Kuhl Heiner Mayer Yvonne Heitmann Katja Gehring Isabel Migdoll Alexander M Kube Michael Knaust Florian Geider Klaus Reinhardt Richard |
| author_facet | Kuhl Heiner Mayer Yvonne Heitmann Katja Gehring Isabel Migdoll Alexander M Kube Michael Knaust Florian Geider Klaus Reinhardt Richard |
| author_sort | Kuhl Heiner |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The genus <it>Erwinia </it>includes plant-associated pathogenic and non-pathogenic <it>Enterobacteria</it>. Important pathogens such as <it>Erwinia amylovora</it>, the causative agent of fire blight and <it>E. pyrifoliae </it>causing bacterial shoot blight of pear in Asia belong to this genus. The species <it>E. tasmaniensis </it>and <it>E. billingiae </it>are epiphytic bacteria and may represent antagonists for biocontrol of fire blight. The presence of genes that are putatively involved in virulence in <it>E. amylovora </it>and <it>E. pyrifoliae </it>is of special interest for these species in consequence.</p> <p>Results</p> <p>Here we provide the complete genome sequences of the pathogenic <it>E. pyrifoliae </it>strain Ep1/96 with a size of 4.1 Mb and of the non-pathogenic species <it>E. billingiae </it>strain Eb661 with a size of 5.4 Mb, <it>de novo </it>determined by conventional Sanger sequencing and next generation sequencing techniques. Genome comparison reveals large inversions resulting from homologous recombination events. Furthermore, comparison of deduced proteins highlights a relation of <it>E. billingiae </it>strain Eb661 to <it>E. tasmaniensis </it>strain Et1/99 and a distance to <it>E. pyrifoliae </it>for the overall gene content as well as for the presence of encoded proteins representing virulence factors for the pathogenic species. Pathogenicity of <it>E. pyrifoliae </it>is supposed to have evolved by accumulation of potential virulence factors. <it>E. pyrifoliae </it>carries factors for type III secretion and cell invasion. Other genes described as virulence factors for <it>E. amylovora </it>are involved in the production of exopolysaccharides, the utilization of plant metabolites such as sorbitol and sucrose. Some virulence-associated genes of the pathogenic species are present in <it>E. tasmaniensis </it>but mostly absent in <it>E. billingiae</it>.</p> <p>Conclusion</p> <p>The data of the genome analyses correspond to the pathogenic lifestyle of <it>E. pyrifoliae </it>and underlines the epiphytic localization of <it>E. tasmaniensis </it>and <it>E. billingiae </it>as a saprophyte<it/>.</p> |
| first_indexed | 2024-04-13T13:05:53Z |
| format | Article |
| id | doaj.art-0cb1ad40668941dca54ed71856b19255 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-13T13:05:53Z |
| publishDate | 2010-06-01 |
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| series | BMC Genomics |
| spelling | doaj.art-0cb1ad40668941dca54ed71856b192552022-12-22T02:45:47ZengBMCBMC Genomics1471-21642010-06-0111139310.1186/1471-2164-11-393Genome comparison of the epiphytic bacteria <it>Erwinia billingiae </it>and <it>E. tasmaniensis </it>with the pear pathogen <it>E. pyrifoliae</it>Kuhl HeinerMayer YvonneHeitmann KatjaGehring IsabelMigdoll Alexander MKube MichaelKnaust FlorianGeider KlausReinhardt Richard<p>Abstract</p> <p>Background</p> <p>The genus <it>Erwinia </it>includes plant-associated pathogenic and non-pathogenic <it>Enterobacteria</it>. Important pathogens such as <it>Erwinia amylovora</it>, the causative agent of fire blight and <it>E. pyrifoliae </it>causing bacterial shoot blight of pear in Asia belong to this genus. The species <it>E. tasmaniensis </it>and <it>E. billingiae </it>are epiphytic bacteria and may represent antagonists for biocontrol of fire blight. The presence of genes that are putatively involved in virulence in <it>E. amylovora </it>and <it>E. pyrifoliae </it>is of special interest for these species in consequence.</p> <p>Results</p> <p>Here we provide the complete genome sequences of the pathogenic <it>E. pyrifoliae </it>strain Ep1/96 with a size of 4.1 Mb and of the non-pathogenic species <it>E. billingiae </it>strain Eb661 with a size of 5.4 Mb, <it>de novo </it>determined by conventional Sanger sequencing and next generation sequencing techniques. Genome comparison reveals large inversions resulting from homologous recombination events. Furthermore, comparison of deduced proteins highlights a relation of <it>E. billingiae </it>strain Eb661 to <it>E. tasmaniensis </it>strain Et1/99 and a distance to <it>E. pyrifoliae </it>for the overall gene content as well as for the presence of encoded proteins representing virulence factors for the pathogenic species. Pathogenicity of <it>E. pyrifoliae </it>is supposed to have evolved by accumulation of potential virulence factors. <it>E. pyrifoliae </it>carries factors for type III secretion and cell invasion. Other genes described as virulence factors for <it>E. amylovora </it>are involved in the production of exopolysaccharides, the utilization of plant metabolites such as sorbitol and sucrose. Some virulence-associated genes of the pathogenic species are present in <it>E. tasmaniensis </it>but mostly absent in <it>E. billingiae</it>.</p> <p>Conclusion</p> <p>The data of the genome analyses correspond to the pathogenic lifestyle of <it>E. pyrifoliae </it>and underlines the epiphytic localization of <it>E. tasmaniensis </it>and <it>E. billingiae </it>as a saprophyte<it/>.</p>http://www.biomedcentral.com/1471-2164/11/393 |
| spellingShingle | Kuhl Heiner Mayer Yvonne Heitmann Katja Gehring Isabel Migdoll Alexander M Kube Michael Knaust Florian Geider Klaus Reinhardt Richard Genome comparison of the epiphytic bacteria <it>Erwinia billingiae </it>and <it>E. tasmaniensis </it>with the pear pathogen <it>E. pyrifoliae</it> BMC Genomics |
| title | Genome comparison of the epiphytic bacteria <it>Erwinia billingiae </it>and <it>E. tasmaniensis </it>with the pear pathogen <it>E. pyrifoliae</it> |
| title_full | Genome comparison of the epiphytic bacteria <it>Erwinia billingiae </it>and <it>E. tasmaniensis </it>with the pear pathogen <it>E. pyrifoliae</it> |
| title_fullStr | Genome comparison of the epiphytic bacteria <it>Erwinia billingiae </it>and <it>E. tasmaniensis </it>with the pear pathogen <it>E. pyrifoliae</it> |
| title_full_unstemmed | Genome comparison of the epiphytic bacteria <it>Erwinia billingiae </it>and <it>E. tasmaniensis </it>with the pear pathogen <it>E. pyrifoliae</it> |
| title_short | Genome comparison of the epiphytic bacteria <it>Erwinia billingiae </it>and <it>E. tasmaniensis </it>with the pear pathogen <it>E. pyrifoliae</it> |
| title_sort | genome comparison of the epiphytic bacteria it erwinia billingiae it and it e tasmaniensis it with the pear pathogen it e pyrifoliae it |
| url | http://www.biomedcentral.com/1471-2164/11/393 |
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