Detection of hydrogen peroxide-producing <it>Lactobacillus</it> species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women
<p>Abstract</p> <p>Background</p> <p>The presence of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) producing <it>Lactobacillus</it> in the vagina may play a role in controlling genital HIV-1 shedding. Sensitive molecular methods impr...
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| Format: | Article |
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BMC
2012-08-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | http://www.biomedcentral.com/1471-2334/12/188 |
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| author | Balkus Jennifer E Mitchell Caroline Agnew Kathy Liu Congzhou Fiedler Tina Cohn Susan E Luque Amneris Coombs Robert Fredricks David N Hitti Jane |
| author_facet | Balkus Jennifer E Mitchell Caroline Agnew Kathy Liu Congzhou Fiedler Tina Cohn Susan E Luque Amneris Coombs Robert Fredricks David N Hitti Jane |
| author_sort | Balkus Jennifer E |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The presence of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) producing <it>Lactobacillus</it> in the vagina may play a role in controlling genital HIV-1 shedding. Sensitive molecular methods improve our ability to characterize the vaginal microbiota; however, they cannot characterize phenotype. We assessed the concordance of H<sub>2</sub>O<sub>2</sub>-producing <it>Lactobacillus</it> detected by culture with quantitative PCR (qPCR) detection of <it>Lactobacillus</it> species commonly assumed to be H<sub>2</sub>O<sub>2</sub>-producers.</p> <p>Methods</p> <p>Samples were collected as part of a prospective cohort study of HIV-1 seropositive US women. Cervicovaginal lavage specimens were tested for <it>L. crispatus</it> and <it>L. jensenii</it> using 16S rRNA gene qPCR assays. Vaginal swabs were cultured for <it>Lactobacillus</it> and tested for H<sub>2</sub>O<sub>2</sub>-production. We calculated a kappa statistic to assess concordance between culture and qPCR.</p> <p>Results</p> <p>Culture and qPCR results were available for 376 visits from 57 women. Lactobacilli were detected by culture at 308 (82%) visits, of which 233 of 308 (76%) produced H<sub>2</sub>O<sub>2</sub>. <it>L. crispatus</it> and/or <it>L. jensenii</it> were detected at 215 (57%) visits. Concordance between detection of <it>L. crispatus</it> and/or <it>L. jensenii</it> by qPCR and H<sub>2</sub>O<sub>2</sub>-producing <it>Lactobacillus</it> by culture was 75% (kappa = 0.45).</p> <p>Conclusions</p> <p>Among HIV-1 seropositive women, there was a moderate level of concordance between H<sub>2</sub>O<sub>2</sub>-producing <it>Lactobacillus</it> detected by culture and the presence of <it>L. crispatus</it> and/or <it>L. jensenii</it> by qPCR. However, one-quarter of samples with growth of H<sub>2</sub>O<sub>2</sub>-producing lactobacilli did not have <it>L. crispatus</it> or <it>L. jensenii</it> detected by qPCR. This discordance may be due to the presence of other H<sub>2</sub>O<sub>2</sub>-producing <it>Lactobacillus</it> species.</p> |
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| spelling | doaj.art-0cb2865cea764182b9cfee399babe6192022-12-21T22:00:24ZengBMCBMC Infectious Diseases1471-23342012-08-0112118810.1186/1471-2334-12-188Detection of hydrogen peroxide-producing <it>Lactobacillus</it> species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive womenBalkus Jennifer EMitchell CarolineAgnew KathyLiu CongzhouFiedler TinaCohn Susan ELuque AmnerisCoombs RobertFredricks David NHitti Jane<p>Abstract</p> <p>Background</p> <p>The presence of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) producing <it>Lactobacillus</it> in the vagina may play a role in controlling genital HIV-1 shedding. Sensitive molecular methods improve our ability to characterize the vaginal microbiota; however, they cannot characterize phenotype. We assessed the concordance of H<sub>2</sub>O<sub>2</sub>-producing <it>Lactobacillus</it> detected by culture with quantitative PCR (qPCR) detection of <it>Lactobacillus</it> species commonly assumed to be H<sub>2</sub>O<sub>2</sub>-producers.</p> <p>Methods</p> <p>Samples were collected as part of a prospective cohort study of HIV-1 seropositive US women. Cervicovaginal lavage specimens were tested for <it>L. crispatus</it> and <it>L. jensenii</it> using 16S rRNA gene qPCR assays. Vaginal swabs were cultured for <it>Lactobacillus</it> and tested for H<sub>2</sub>O<sub>2</sub>-production. We calculated a kappa statistic to assess concordance between culture and qPCR.</p> <p>Results</p> <p>Culture and qPCR results were available for 376 visits from 57 women. Lactobacilli were detected by culture at 308 (82%) visits, of which 233 of 308 (76%) produced H<sub>2</sub>O<sub>2</sub>. <it>L. crispatus</it> and/or <it>L. jensenii</it> were detected at 215 (57%) visits. Concordance between detection of <it>L. crispatus</it> and/or <it>L. jensenii</it> by qPCR and H<sub>2</sub>O<sub>2</sub>-producing <it>Lactobacillus</it> by culture was 75% (kappa = 0.45).</p> <p>Conclusions</p> <p>Among HIV-1 seropositive women, there was a moderate level of concordance between H<sub>2</sub>O<sub>2</sub>-producing <it>Lactobacillus</it> detected by culture and the presence of <it>L. crispatus</it> and/or <it>L. jensenii</it> by qPCR. However, one-quarter of samples with growth of H<sub>2</sub>O<sub>2</sub>-producing lactobacilli did not have <it>L. crispatus</it> or <it>L. jensenii</it> detected by qPCR. This discordance may be due to the presence of other H<sub>2</sub>O<sub>2</sub>-producing <it>Lactobacillus</it> species.</p>http://www.biomedcentral.com/1471-2334/12/188<it>Lactobacillus</it><it>Lactobacillus crispatus</it><it>Lactobacillus jensenii</it>Bacterial vaginosisCultureHydrogen peroxide16S rRNA gene |
| spellingShingle | Balkus Jennifer E Mitchell Caroline Agnew Kathy Liu Congzhou Fiedler Tina Cohn Susan E Luque Amneris Coombs Robert Fredricks David N Hitti Jane Detection of hydrogen peroxide-producing <it>Lactobacillus</it> species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women BMC Infectious Diseases <it>Lactobacillus</it> <it>Lactobacillus crispatus</it> <it>Lactobacillus jensenii</it> Bacterial vaginosis Culture Hydrogen peroxide 16S rRNA gene |
| title | Detection of hydrogen peroxide-producing <it>Lactobacillus</it> species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women |
| title_full | Detection of hydrogen peroxide-producing <it>Lactobacillus</it> species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women |
| title_fullStr | Detection of hydrogen peroxide-producing <it>Lactobacillus</it> species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women |
| title_full_unstemmed | Detection of hydrogen peroxide-producing <it>Lactobacillus</it> species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women |
| title_short | Detection of hydrogen peroxide-producing <it>Lactobacillus</it> species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women |
| title_sort | detection of hydrogen peroxide producing it lactobacillus it species in the vagina a comparison of culture and quantitative pcr among hiv 1 seropositive women |
| topic | <it>Lactobacillus</it> <it>Lactobacillus crispatus</it> <it>Lactobacillus jensenii</it> Bacterial vaginosis Culture Hydrogen peroxide 16S rRNA gene |
| url | http://www.biomedcentral.com/1471-2334/12/188 |
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