Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea
Anthocyanins confer the wide range of colors for plants and also play beneficial health roles as potentially protective factors against heart disease and cancer. Brassica juncea is cultivated as an edible oil resource and vegetable crop worldwide, thus elucidating the anthocyanin biosynthetic pathwa...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-05-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311920631720 |
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| author | Da-wei ZHANG Li-li LIU Ding-gang ZHOU Xian-jun LIU Zhong-song LIU Ming-li YAN |
| author_facet | Da-wei ZHANG Li-li LIU Ding-gang ZHOU Xian-jun LIU Zhong-song LIU Ming-li YAN |
| author_sort | Da-wei ZHANG |
| collection | DOAJ |
| description | Anthocyanins confer the wide range of colors for plants and also play beneficial health roles as potentially protective factors against heart disease and cancer. Brassica juncea is cultivated as an edible oil resource and vegetable crop worldwide, thus elucidating the anthocyanin biosynthetic pathway would be helpful to improve the nutritional quality of Brassica juncea through the breeding and cultivating of high anthocyanin content varieties. Herein, 129 genes in B. juncea were identified as orthologs of 41 anthocyanin biosynthetic genes (ABGs) in Arabidopsis thaliana by comparative genomic analyses. The B. juncea ABGs have expanded by whole genome triplication and subsequent allopolyploidizatoin, but lost mainly during the whole genome triplication between B. rapa/B. nigra and A. thaliana, rather than the allopolyploidization process between B. juncea and B. rapa/B. nigra, leading to different copy numbers retention of A. thaliana homologous genes. Although the overall expansion levels ABGs were similar to the whole genome, more negative regulatory genes were retained in the anthocyanin biosynthesis regulatory system. Transcriptional analysis of B. juncea with different anthocyanin accumulation showed that BjDFR, BjTT19, BjTT8 are significantly up-regulated in plants with purple leaves as compared with green leaves. The overexpression of BjTT8 and these target genes which were involved in late anthocyanin biosynthesis and transport might account for increasing levels of anthocyanin accumulation in purple leaves. Our results could promote the understanding of the genetic mechanism of anthocyanin biosynthesis in B. juncea. |
| first_indexed | 2024-12-17T00:11:03Z |
| format | Article |
| id | doaj.art-0cb46668b9d347009c8d18436d322501 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-17T00:11:03Z |
| publishDate | 2020-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-0cb46668b9d347009c8d18436d3225012022-12-21T22:10:50ZengElsevierJournal of Integrative Agriculture2095-31192020-05-0119512501260Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica junceaDa-wei ZHANG0Li-li LIU1Ding-gang ZHOU2Xian-jun LIU3Zhong-song LIU4Ming-li YAN5School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, P.R.China; Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, Xiangtan 411201, P.R.ChinaSchool of Life Science, Hunan University of Science and Technology, Xiangtan 411201, P.R.China; Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, Xiangtan 411201, P.R.ChinaSchool of Life Science, Hunan University of Science and Technology, Xiangtan 411201, P.R.China; Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, Xiangtan 411201, P.R.ChinaCollege of Life Sciences, Resources and Environment Sciences, Yichun University, Yichun 333600, P.R.ChinaOilseed Research Institute, Hunan Agricultural University, Changsha 410128, P.R.ChinaSchool of Life Science, Hunan University of Science and Technology, Xiangtan 411201, P.R.China; Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, Xiangtan 411201, P.R.China; Correspondence YAN Ming-li, Tel/Fax: +86-731-58291416Anthocyanins confer the wide range of colors for plants and also play beneficial health roles as potentially protective factors against heart disease and cancer. Brassica juncea is cultivated as an edible oil resource and vegetable crop worldwide, thus elucidating the anthocyanin biosynthetic pathway would be helpful to improve the nutritional quality of Brassica juncea through the breeding and cultivating of high anthocyanin content varieties. Herein, 129 genes in B. juncea were identified as orthologs of 41 anthocyanin biosynthetic genes (ABGs) in Arabidopsis thaliana by comparative genomic analyses. The B. juncea ABGs have expanded by whole genome triplication and subsequent allopolyploidizatoin, but lost mainly during the whole genome triplication between B. rapa/B. nigra and A. thaliana, rather than the allopolyploidization process between B. juncea and B. rapa/B. nigra, leading to different copy numbers retention of A. thaliana homologous genes. Although the overall expansion levels ABGs were similar to the whole genome, more negative regulatory genes were retained in the anthocyanin biosynthesis regulatory system. Transcriptional analysis of B. juncea with different anthocyanin accumulation showed that BjDFR, BjTT19, BjTT8 are significantly up-regulated in plants with purple leaves as compared with green leaves. The overexpression of BjTT8 and these target genes which were involved in late anthocyanin biosynthesis and transport might account for increasing levels of anthocyanin accumulation in purple leaves. Our results could promote the understanding of the genetic mechanism of anthocyanin biosynthesis in B. juncea.http://www.sciencedirect.com/science/article/pii/S2095311920631720anthocyanin biosynthetic genesB. junceacomparative genomic analysistranscriptomeleaf color |
| spellingShingle | Da-wei ZHANG Li-li LIU Ding-gang ZHOU Xian-jun LIU Zhong-song LIU Ming-li YAN Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea Journal of Integrative Agriculture anthocyanin biosynthetic genes B. juncea comparative genomic analysis transcriptome leaf color |
| title | Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea |
| title_full | Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea |
| title_fullStr | Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea |
| title_full_unstemmed | Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea |
| title_short | Genome-wide identification and expression analysis of anthocyanin biosynthetic genes in Brassica juncea |
| title_sort | genome wide identification and expression analysis of anthocyanin biosynthetic genes in brassica juncea |
| topic | anthocyanin biosynthetic genes B. juncea comparative genomic analysis transcriptome leaf color |
| url | http://www.sciencedirect.com/science/article/pii/S2095311920631720 |
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