Effect of citric acid, tetracycline, and doxycycline on instrumented periodontally involved root surfaces: A SEM study

Background: A surface smear layer consisting of organic and inorganic material is formed on the root surface following mechanical instrumentation and may inhibit the formation of new connective tissue attachment to the root surface. Modification of the tooth surface by root conditioning has resulted...

Full description

Bibliographic Details
Main Authors: Gurparkash Singh Chahal, Kamalpreet Chhina, Vipin Chhabra, Rakhi Bhatnagar, Amna Chahal
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2014-01-01
Series:Journal of Indian Society of Periodontology
Subjects:
Online Access:http://www.jisponline.com/article.asp?issn=0972-124X;year=2014;volume=18;issue=1;spage=32;epage=37;aulast=Chahal
_version_ 1819155464874622976
author Gurparkash Singh Chahal
Kamalpreet Chhina
Vipin Chhabra
Rakhi Bhatnagar
Amna Chahal
author_facet Gurparkash Singh Chahal
Kamalpreet Chhina
Vipin Chhabra
Rakhi Bhatnagar
Amna Chahal
author_sort Gurparkash Singh Chahal
collection DOAJ
description Background: A surface smear layer consisting of organic and inorganic material is formed on the root surface following mechanical instrumentation and may inhibit the formation of new connective tissue attachment to the root surface. Modification of the tooth surface by root conditioning has resulted in improved connective tissue attachment and has advanced the goal of reconstructive periodontal treatment. Aim: The aim of this study was to compare the effects of citric acid, tetracycline, and doxycycline on the instrumented periodontally involved root surfaces in vitro using a scanning electron microscope. Settings and Design: A total of 45 dentin samples obtained from 15 extracted, scaled, and root planed teeth were divided into three groups. Materials and Methods: The root conditioning agents were applied with cotton pellets using the "Passive burnishing technique" for 5 minutes. The samples were then examined by the scanning electron microscope. Statistical Analysis Used: The statistical analysis was carried out using Statistical Package for Social Sciences (SPSS Inc., Chicago, IL, version 15.0 for Windows). For all quantitative variables means and standard deviations were calculated and compared. For more than two groups ANOVA was applied. For multiple comparisons post hoc tests with Bonferroni correction was used. Results: Upon statistical analysis the root conditioning agents used in this study were found to be effective in removing the smear layer, uncovering and widening the dentin tubules and unmasking the dentin collagen matrix. Conclusion: Tetracycline HCl was found to be the best root conditioner among the three agents used.
first_indexed 2024-12-22T15:37:24Z
format Article
id doaj.art-0cb53cfda07548039267945e3fbd9ea3
institution Directory Open Access Journal
issn 0972-124X
language English
last_indexed 2024-12-22T15:37:24Z
publishDate 2014-01-01
publisher Wolters Kluwer Medknow Publications
record_format Article
series Journal of Indian Society of Periodontology
spelling doaj.art-0cb53cfda07548039267945e3fbd9ea32022-12-21T18:21:13ZengWolters Kluwer Medknow PublicationsJournal of Indian Society of Periodontology0972-124X2014-01-01181323710.4103/0972-124X.128196Effect of citric acid, tetracycline, and doxycycline on instrumented periodontally involved root surfaces: A SEM studyGurparkash Singh ChahalKamalpreet ChhinaVipin ChhabraRakhi BhatnagarAmna ChahalBackground: A surface smear layer consisting of organic and inorganic material is formed on the root surface following mechanical instrumentation and may inhibit the formation of new connective tissue attachment to the root surface. Modification of the tooth surface by root conditioning has resulted in improved connective tissue attachment and has advanced the goal of reconstructive periodontal treatment. Aim: The aim of this study was to compare the effects of citric acid, tetracycline, and doxycycline on the instrumented periodontally involved root surfaces in vitro using a scanning electron microscope. Settings and Design: A total of 45 dentin samples obtained from 15 extracted, scaled, and root planed teeth were divided into three groups. Materials and Methods: The root conditioning agents were applied with cotton pellets using the "Passive burnishing technique" for 5 minutes. The samples were then examined by the scanning electron microscope. Statistical Analysis Used: The statistical analysis was carried out using Statistical Package for Social Sciences (SPSS Inc., Chicago, IL, version 15.0 for Windows). For all quantitative variables means and standard deviations were calculated and compared. For more than two groups ANOVA was applied. For multiple comparisons post hoc tests with Bonferroni correction was used. Results: Upon statistical analysis the root conditioning agents used in this study were found to be effective in removing the smear layer, uncovering and widening the dentin tubules and unmasking the dentin collagen matrix. Conclusion: Tetracycline HCl was found to be the best root conditioner among the three agents used.http://www.jisponline.com/article.asp?issn=0972-124X;year=2014;volume=18;issue=1;spage=32;epage=37;aulast=ChahalCitric acidscanning electron microscopetetracycline hydrochloride
spellingShingle Gurparkash Singh Chahal
Kamalpreet Chhina
Vipin Chhabra
Rakhi Bhatnagar
Amna Chahal
Effect of citric acid, tetracycline, and doxycycline on instrumented periodontally involved root surfaces: A SEM study
Journal of Indian Society of Periodontology
Citric acid
scanning electron microscope
tetracycline hydrochloride
title Effect of citric acid, tetracycline, and doxycycline on instrumented periodontally involved root surfaces: A SEM study
title_full Effect of citric acid, tetracycline, and doxycycline on instrumented periodontally involved root surfaces: A SEM study
title_fullStr Effect of citric acid, tetracycline, and doxycycline on instrumented periodontally involved root surfaces: A SEM study
title_full_unstemmed Effect of citric acid, tetracycline, and doxycycline on instrumented periodontally involved root surfaces: A SEM study
title_short Effect of citric acid, tetracycline, and doxycycline on instrumented periodontally involved root surfaces: A SEM study
title_sort effect of citric acid tetracycline and doxycycline on instrumented periodontally involved root surfaces a sem study
topic Citric acid
scanning electron microscope
tetracycline hydrochloride
url http://www.jisponline.com/article.asp?issn=0972-124X;year=2014;volume=18;issue=1;spage=32;epage=37;aulast=Chahal
work_keys_str_mv AT gurparkashsinghchahal effectofcitricacidtetracyclineanddoxycyclineoninstrumentedperiodontallyinvolvedrootsurfacesasemstudy
AT kamalpreetchhina effectofcitricacidtetracyclineanddoxycyclineoninstrumentedperiodontallyinvolvedrootsurfacesasemstudy
AT vipinchhabra effectofcitricacidtetracyclineanddoxycyclineoninstrumentedperiodontallyinvolvedrootsurfacesasemstudy
AT rakhibhatnagar effectofcitricacidtetracyclineanddoxycyclineoninstrumentedperiodontallyinvolvedrootsurfacesasemstudy
AT amnachahal effectofcitricacidtetracyclineanddoxycyclineoninstrumentedperiodontallyinvolvedrootsurfacesasemstudy