Optimum conditions for Inulinase production by Aspergillus niger using solid state fermentation
Thirty local fungal isolates according to Aspergillus niger were screened for Inulinase production on synthetic solid medium depending on inulin hydrolysis appear as clear zone around fungal colony. Semi-quantitative screening was performed to select the most efficient isolate for inulinase producti...
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| Format: | Article |
| Language: | Arabic |
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College of Science for Women, University of Baghdad
2015-06-01
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| Series: | Baghdad Science Journal |
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| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2071 |
| _version_ | 1818458787560816640 |
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| author | Baghdad Science Journal |
| author_facet | Baghdad Science Journal |
| author_sort | Baghdad Science Journal |
| collection | DOAJ |
| description | Thirty local fungal isolates according to Aspergillus niger were screened for Inulinase production on synthetic solid medium depending on inulin hydrolysis appear as clear zone around fungal colony. Semi-quantitative screening was performed to select the most efficient isolate for inulinase production. the most efficient isolate was AN20. The optimum condition for enzyme production from A. niger isolate was determined by busing a medium composed of sugar cane moisten with corn steep liquor 5;5 (v/w) at initial pH 5.0 for 96 hours at 30 0C . Enzyme productivity was tested for each of the yeast Kluyveromyces marxianus, the fungus A. niger AN20 and for a mixed culture of A. niger and K. marxianus. The productivity of A. niger gave the highest specific activity of 153 U/mg, as compared with K. marxianus which gave 86 U/mg. |
| first_indexed | 2024-12-14T23:04:01Z |
| format | Article |
| id | doaj.art-0cba8ebd44ce4c4d9ea793ec1164dbd4 |
| institution | Directory Open Access Journal |
| issn | 2078-8665 2411-7986 |
| language | Arabic |
| last_indexed | 2024-12-14T23:04:01Z |
| publishDate | 2015-06-01 |
| publisher | College of Science for Women, University of Baghdad |
| record_format | Article |
| series | Baghdad Science Journal |
| spelling | doaj.art-0cba8ebd44ce4c4d9ea793ec1164dbd42022-12-21T22:44:22ZaraCollege of Science for Women, University of BaghdadBaghdad Science Journal2078-86652411-79862015-06-0112210.21123/bsj.12.2.307-316Optimum conditions for Inulinase production by Aspergillus niger using solid state fermentationBaghdad Science JournalThirty local fungal isolates according to Aspergillus niger were screened for Inulinase production on synthetic solid medium depending on inulin hydrolysis appear as clear zone around fungal colony. Semi-quantitative screening was performed to select the most efficient isolate for inulinase production. the most efficient isolate was AN20. The optimum condition for enzyme production from A. niger isolate was determined by busing a medium composed of sugar cane moisten with corn steep liquor 5;5 (v/w) at initial pH 5.0 for 96 hours at 30 0C . Enzyme productivity was tested for each of the yeast Kluyveromyces marxianus, the fungus A. niger AN20 and for a mixed culture of A. niger and K. marxianus. The productivity of A. niger gave the highest specific activity of 153 U/mg, as compared with K. marxianus which gave 86 U/mg.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2071Inulinase , Aspergillus niger; Kluyveromyces marxianus |
| spellingShingle | Baghdad Science Journal Optimum conditions for Inulinase production by Aspergillus niger using solid state fermentation Baghdad Science Journal Inulinase , Aspergillus niger; Kluyveromyces marxianus |
| title | Optimum conditions for Inulinase production by Aspergillus niger using solid state fermentation |
| title_full | Optimum conditions for Inulinase production by Aspergillus niger using solid state fermentation |
| title_fullStr | Optimum conditions for Inulinase production by Aspergillus niger using solid state fermentation |
| title_full_unstemmed | Optimum conditions for Inulinase production by Aspergillus niger using solid state fermentation |
| title_short | Optimum conditions for Inulinase production by Aspergillus niger using solid state fermentation |
| title_sort | optimum conditions for inulinase production by aspergillus niger using solid state fermentation |
| topic | Inulinase , Aspergillus niger; Kluyveromyces marxianus |
| url | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2071 |
| work_keys_str_mv | AT baghdadsciencejournal optimumconditionsforinulinaseproductionbyaspergillusnigerusingsolidstatefermentation |