Impact of Enzymatic Hydrolysis and Microfluidization on the Techno-Functionality of Oat Bran in Suspension and Acid Milk Gel Models

Oat bran is a nutritionally rich ingredient, but it is underutilized in semi-moist and liquid foods due to technological issues such as high viscosity and sliminess. The aim of this work was to improve the technological properties of oat bran concentrate (OBC) in high-moisture food applications by e...

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Main Authors: Natalia Rosa-Sibakov, Maria Julia de Oliveira Carvalho, Martina Lille, Emilia Nordlund
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/11/2/228
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author Natalia Rosa-Sibakov
Maria Julia de Oliveira Carvalho
Martina Lille
Emilia Nordlund
author_facet Natalia Rosa-Sibakov
Maria Julia de Oliveira Carvalho
Martina Lille
Emilia Nordlund
author_sort Natalia Rosa-Sibakov
collection DOAJ
description Oat bran is a nutritionally rich ingredient, but it is underutilized in semi-moist and liquid foods due to technological issues such as high viscosity and sliminess. The aim of this work was to improve the technological properties of oat bran concentrate (OBC) in high-moisture food applications by enzymatic and mechanical treatments. OBC was hydrolyzed with β-glucanase (OBC-Hyd) and the water-soluble fraction (OBC-Sol) was separated. OBC, OBC-Hyd and OBC-Sol were further microfluidized at 5% dry matter content. Enzymatic treatment and microfluidization of OBC reduced the molecular weight (Mw) of β-glucan from 2748 kDa to 893 and 350 kDa, respectively, as well as the average particle size of OBC (3.4 and 35 times, respectively). Both treatments increased the extractability of the soluble compounds from the OBC samples (up to 80%) and affected their water retention capacity. OBC in suspension had very high viscosity (969 mPa·s) when heated, which decreased after both enzyme and microfluidization treatments. The colloidal stability of the OBC in suspension was improved, especially after microfluidization. The addition of OBC samples to acid milk gels decreased syneresis, improved the water holding capacity and softened the texture. The changes in the suspension and gel characteristics were linked with reduced β-glucan Mw and OBC particle size.
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spelling doaj.art-0cbd1813c7c54535ab49d5715fbca1fc2023-11-23T13:45:49ZengMDPI AGFoods2304-81582022-01-0111222810.3390/foods11020228Impact of Enzymatic Hydrolysis and Microfluidization on the Techno-Functionality of Oat Bran in Suspension and Acid Milk Gel ModelsNatalia Rosa-Sibakov0Maria Julia de Oliveira Carvalho1Martina Lille2Emilia Nordlund3VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 Espoo, FinlandVTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 Espoo, FinlandVTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 Espoo, FinlandVTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 Espoo, FinlandOat bran is a nutritionally rich ingredient, but it is underutilized in semi-moist and liquid foods due to technological issues such as high viscosity and sliminess. The aim of this work was to improve the technological properties of oat bran concentrate (OBC) in high-moisture food applications by enzymatic and mechanical treatments. OBC was hydrolyzed with β-glucanase (OBC-Hyd) and the water-soluble fraction (OBC-Sol) was separated. OBC, OBC-Hyd and OBC-Sol were further microfluidized at 5% dry matter content. Enzymatic treatment and microfluidization of OBC reduced the molecular weight (Mw) of β-glucan from 2748 kDa to 893 and 350 kDa, respectively, as well as the average particle size of OBC (3.4 and 35 times, respectively). Both treatments increased the extractability of the soluble compounds from the OBC samples (up to 80%) and affected their water retention capacity. OBC in suspension had very high viscosity (969 mPa·s) when heated, which decreased after both enzyme and microfluidization treatments. The colloidal stability of the OBC in suspension was improved, especially after microfluidization. The addition of OBC samples to acid milk gels decreased syneresis, improved the water holding capacity and softened the texture. The changes in the suspension and gel characteristics were linked with reduced β-glucan Mw and OBC particle size.https://www.mdpi.com/2304-8158/11/2/228oat branβ-glucanβ-glucanasemechanical treatmentacid milk gel
spellingShingle Natalia Rosa-Sibakov
Maria Julia de Oliveira Carvalho
Martina Lille
Emilia Nordlund
Impact of Enzymatic Hydrolysis and Microfluidization on the Techno-Functionality of Oat Bran in Suspension and Acid Milk Gel Models
Foods
oat bran
β-glucan
β-glucanase
mechanical treatment
acid milk gel
title Impact of Enzymatic Hydrolysis and Microfluidization on the Techno-Functionality of Oat Bran in Suspension and Acid Milk Gel Models
title_full Impact of Enzymatic Hydrolysis and Microfluidization on the Techno-Functionality of Oat Bran in Suspension and Acid Milk Gel Models
title_fullStr Impact of Enzymatic Hydrolysis and Microfluidization on the Techno-Functionality of Oat Bran in Suspension and Acid Milk Gel Models
title_full_unstemmed Impact of Enzymatic Hydrolysis and Microfluidization on the Techno-Functionality of Oat Bran in Suspension and Acid Milk Gel Models
title_short Impact of Enzymatic Hydrolysis and Microfluidization on the Techno-Functionality of Oat Bran in Suspension and Acid Milk Gel Models
title_sort impact of enzymatic hydrolysis and microfluidization on the techno functionality of oat bran in suspension and acid milk gel models
topic oat bran
β-glucan
β-glucanase
mechanical treatment
acid milk gel
url https://www.mdpi.com/2304-8158/11/2/228
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AT martinalille impactofenzymatichydrolysisandmicrofluidizationonthetechnofunctionalityofoatbraninsuspensionandacidmilkgelmodels
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