Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis
A new method using a magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was compared with sputum smear microscopy (SSM) for the detection of pulmonary tuberculosis (PTB) in sputum samples. Studies were made to compare the NCBA against SSM using sputum samples collected from PTB patient...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/8/4/128 |
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| author | Cristina Gordillo-Marroquín Anaximandro Gómez-Velasco Héctor J. Sánchez-Pérez Kasey Pryg John Shinners Nathan Murray Sergio G. Muñoz-Jiménez Allied Bencomo-Alerm Adriana Gómez-Bustamante Letisia Jonapá-Gómez Natán Enríquez-Ríos Miguel Martín Natalia Romero-Sandoval Evangelyn C. Alocilja |
| author_facet | Cristina Gordillo-Marroquín Anaximandro Gómez-Velasco Héctor J. Sánchez-Pérez Kasey Pryg John Shinners Nathan Murray Sergio G. Muñoz-Jiménez Allied Bencomo-Alerm Adriana Gómez-Bustamante Letisia Jonapá-Gómez Natán Enríquez-Ríos Miguel Martín Natalia Romero-Sandoval Evangelyn C. Alocilja |
| author_sort | Cristina Gordillo-Marroquín |
| collection | DOAJ |
| description | A new method using a magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was compared with sputum smear microscopy (SSM) for the detection of pulmonary tuberculosis (PTB) in sputum samples. Studies were made to compare the NCBA against SSM using sputum samples collected from PTB patients prior to receiving treatment. Experiments were also conducted to determine the appropriate concentration of glycan-functionalized magnetic nanoparticles (GMNP) used in the NCBA and to evaluate the optimal digestion/decontamination solution to increase the extraction, concentration and detection of acid-fast bacilli (AFB). The optimized NCBA consisted of a 1:1 mixture of 0.4% NaOH and 4% N-acetyl-L-cysteine (NALC) to homogenize the sputum sample. Additionally, 10 mg/mL of GMNP was added to isolate and concentrate the AFB. All TB positive sputum samples were identified with an increased AFB count of 47% compared to SSM, demonstrating GMNP’s ability to extract and concentrate AFB. Results showed that NCBA increased AFB count compared to SSM, improving the grade from “1+„ (in SSM) to “2+„. Extending the finding to paucibacillary cases, there is the likelihood of a “scant„ grade to become “1+„. The assay uses a simple magnet and only costs $0.10/test. NCBA has great potential application in TB control programs. |
| first_indexed | 2024-12-10T12:39:39Z |
| format | Article |
| id | doaj.art-0cc11b8f1cfe426dbccfec430154a9d1 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-12-10T12:39:39Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-0cc11b8f1cfe426dbccfec430154a9d12022-12-22T01:48:34ZengMDPI AGBiosensors2079-63742018-12-018412810.3390/bios8040128bios8040128Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary TuberculosisCristina Gordillo-Marroquín0Anaximandro Gómez-Velasco1Héctor J. Sánchez-Pérez2Kasey Pryg3John Shinners4Nathan Murray5Sergio G. Muñoz-Jiménez6Allied Bencomo-Alerm7Adriana Gómez-Bustamante8Letisia Jonapá-Gómez9Natán Enríquez-Ríos10Miguel Martín11Natalia Romero-Sandoval12Evangelyn C. Alocilja13Health Department, El Colegio de la Frontera Sur (ECOSUR), San Cristobal de Las Casas, Chiapas 29290, MexicoHealth Department, El Colegio de la Frontera Sur (ECOSUR), San Cristobal de Las Casas, Chiapas 29290, MexicoHealth Department, El Colegio de la Frontera Sur (ECOSUR), San Cristobal de Las Casas, Chiapas 29290, MexicoNano-Biosensors Laboratory, Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USANano-Biosensors Laboratory, Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USANano-Biosensors Laboratory, Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USAGlobal Alliance for Rapid Diagnostics. Michigan State University, East Lansing, MI 48824, USAGlobal Alliance for Rapid Diagnostics. Michigan State University, East Lansing, MI 48824, USAState Public Health Laboratory for Chiapas, Tuxtla Gutierrez, Chiapas 29040, MexicoState Public Health Laboratory for Chiapas, Tuxtla Gutierrez, Chiapas 29040, MexicoGlobal Alliance for Rapid Diagnostics. Michigan State University, East Lansing, MI 48824, USAThe Network GRAAL (Grups de Recerca d’America i Africa Llatines)-ECOSUR Node, San Cristobal de Las Casas, Chiapas 29290, MexicoThe Network GRAAL (Grups de Recerca d’America i Africa Llatines)-ECOSUR Node, San Cristobal de Las Casas, Chiapas 29290, MexicoGlobal Alliance for Rapid Diagnostics. Michigan State University, East Lansing, MI 48824, USAA new method using a magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was compared with sputum smear microscopy (SSM) for the detection of pulmonary tuberculosis (PTB) in sputum samples. Studies were made to compare the NCBA against SSM using sputum samples collected from PTB patients prior to receiving treatment. Experiments were also conducted to determine the appropriate concentration of glycan-functionalized magnetic nanoparticles (GMNP) used in the NCBA and to evaluate the optimal digestion/decontamination solution to increase the extraction, concentration and detection of acid-fast bacilli (AFB). The optimized NCBA consisted of a 1:1 mixture of 0.4% NaOH and 4% N-acetyl-L-cysteine (NALC) to homogenize the sputum sample. Additionally, 10 mg/mL of GMNP was added to isolate and concentrate the AFB. All TB positive sputum samples were identified with an increased AFB count of 47% compared to SSM, demonstrating GMNP’s ability to extract and concentrate AFB. Results showed that NCBA increased AFB count compared to SSM, improving the grade from “1+„ (in SSM) to “2+„. Extending the finding to paucibacillary cases, there is the likelihood of a “scant„ grade to become “1+„. The assay uses a simple magnet and only costs $0.10/test. NCBA has great potential application in TB control programs.https://www.mdpi.com/2079-6374/8/4/128<i>Mycobacterium tuberculosis</i>increased sensitivitysputum smear microscopyTB detectionnanotechnologyinfectious disease |
| spellingShingle | Cristina Gordillo-Marroquín Anaximandro Gómez-Velasco Héctor J. Sánchez-Pérez Kasey Pryg John Shinners Nathan Murray Sergio G. Muñoz-Jiménez Allied Bencomo-Alerm Adriana Gómez-Bustamante Letisia Jonapá-Gómez Natán Enríquez-Ríos Miguel Martín Natalia Romero-Sandoval Evangelyn C. Alocilja Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis Biosensors <i>Mycobacterium tuberculosis</i> increased sensitivity sputum smear microscopy TB detection nanotechnology infectious disease |
| title | Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis |
| title_full | Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis |
| title_fullStr | Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis |
| title_full_unstemmed | Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis |
| title_short | Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis |
| title_sort | magnetic nanoparticle based biosensing assay quantitatively enhances acid fast bacilli count in paucibacillary pulmonary tuberculosis |
| topic | <i>Mycobacterium tuberculosis</i> increased sensitivity sputum smear microscopy TB detection nanotechnology infectious disease |
| url | https://www.mdpi.com/2079-6374/8/4/128 |
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