The Roles of Bacteria in Soil Organic Carbon Accumulation under Nitrogen Deposition in <i>Stipa baicalensis</i> Steppe
Grassland soil organic carbon (SOC) accounts for 15.5% of the SOC in reservoirs of terrestrial carbon (C) and is a major component of the global C cycle. Current and future reactive N deposited on grassland soils may alter biogeochemical processes and soil microbes. Microorganisms perform most of th...
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MDPI AG
2020-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/3/326 |
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| author | Jie Qin Hongmei Liu Jianning Zhao Hui Wang Haifang Zhang Dianlin Yang Naiqing Zhang |
| author_facet | Jie Qin Hongmei Liu Jianning Zhao Hui Wang Haifang Zhang Dianlin Yang Naiqing Zhang |
| author_sort | Jie Qin |
| collection | DOAJ |
| description | Grassland soil organic carbon (SOC) accounts for 15.5% of the SOC in reservoirs of terrestrial carbon (C) and is a major component of the global C cycle. Current and future reactive N deposited on grassland soils may alter biogeochemical processes and soil microbes. Microorganisms perform most of the decomposition on Earth and shift SOC accumulation. However, how variation in the identity and composition of the bacterial community influences SOC is far from clear. The objective of this study is to investigate the responses of SOC concentration to multiple rates of N addition as well as the roles of bacteria in SOC accumulation. We studied SOC storage and bacterial community composition under N addition treatments (0, 1.5, 3.0, 5.0, 10.0, 15.0, 20.0, and 30.0 g N·m<sup>-2</sup> yr<sup>−1</sup>) in a 6-yr field experiment in a temperate grassland. We determined the soil inorganic nitrogen concentration and pH in a 0−10 cm soil layer. We used high-throughput genetic sequencing to detect bacteria. N addition led to significant increases in the concentrations of SOC. N addition reduced the soil pH but increased the NO<sub>3</sub>-N and NH<sub>4</sub>-N levels. The bacterial diversity was highest under low nitrogen addition. N addition increased the relative abundance of Proteobacteria, and Proteobacteria became the second dominant phylum under high N addition. Structural equation modeling further revealed that soil pH and bacterial community structure have an impact on SOC under N deposition. Nitrogen-regulated SOC is associated with Proteobacteria and Planctomycetes. These findings suggest that N deposition may alter the SOC content, highlighting the importance of understanding changes in the bacterial community for soil nutrients under N deposition. |
| first_indexed | 2024-04-14T05:25:58Z |
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| id | doaj.art-0cc2791c517944a589f6b5bc805839b1 |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-14T05:25:58Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-0cc2791c517944a589f6b5bc805839b12022-12-22T02:09:58ZengMDPI AGMicroorganisms2076-26072020-02-018332610.3390/microorganisms8030326microorganisms8030326The Roles of Bacteria in Soil Organic Carbon Accumulation under Nitrogen Deposition in <i>Stipa baicalensis</i> SteppeJie Qin0Hongmei Liu1Jianning Zhao2Hui Wang3Haifang Zhang4Dianlin Yang5Naiqing Zhang6Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, MARA, Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Tianjin 300191, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, MARA, Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Tianjin 300191, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, MARA, Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Tianjin 300191, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, MARA, Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Tianjin 300191, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, MARA, Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Tianjin 300191, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, MARA, Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Tianjin 300191, ChinaDepartment of Ecology and Landscape Architecture, Dezhou University, Dezhou 253023, ChinaGrassland soil organic carbon (SOC) accounts for 15.5% of the SOC in reservoirs of terrestrial carbon (C) and is a major component of the global C cycle. Current and future reactive N deposited on grassland soils may alter biogeochemical processes and soil microbes. Microorganisms perform most of the decomposition on Earth and shift SOC accumulation. However, how variation in the identity and composition of the bacterial community influences SOC is far from clear. The objective of this study is to investigate the responses of SOC concentration to multiple rates of N addition as well as the roles of bacteria in SOC accumulation. We studied SOC storage and bacterial community composition under N addition treatments (0, 1.5, 3.0, 5.0, 10.0, 15.0, 20.0, and 30.0 g N·m<sup>-2</sup> yr<sup>−1</sup>) in a 6-yr field experiment in a temperate grassland. We determined the soil inorganic nitrogen concentration and pH in a 0−10 cm soil layer. We used high-throughput genetic sequencing to detect bacteria. N addition led to significant increases in the concentrations of SOC. N addition reduced the soil pH but increased the NO<sub>3</sub>-N and NH<sub>4</sub>-N levels. The bacterial diversity was highest under low nitrogen addition. N addition increased the relative abundance of Proteobacteria, and Proteobacteria became the second dominant phylum under high N addition. Structural equation modeling further revealed that soil pH and bacterial community structure have an impact on SOC under N deposition. Nitrogen-regulated SOC is associated with Proteobacteria and Planctomycetes. These findings suggest that N deposition may alter the SOC content, highlighting the importance of understanding changes in the bacterial community for soil nutrients under N deposition.https://www.mdpi.com/2076-2607/8/3/326nitrogen depositiongrasslandsoil organic carbonbacteriasoil properties |
| spellingShingle | Jie Qin Hongmei Liu Jianning Zhao Hui Wang Haifang Zhang Dianlin Yang Naiqing Zhang The Roles of Bacteria in Soil Organic Carbon Accumulation under Nitrogen Deposition in <i>Stipa baicalensis</i> Steppe Microorganisms nitrogen deposition grassland soil organic carbon bacteria soil properties |
| title | The Roles of Bacteria in Soil Organic Carbon Accumulation under Nitrogen Deposition in <i>Stipa baicalensis</i> Steppe |
| title_full | The Roles of Bacteria in Soil Organic Carbon Accumulation under Nitrogen Deposition in <i>Stipa baicalensis</i> Steppe |
| title_fullStr | The Roles of Bacteria in Soil Organic Carbon Accumulation under Nitrogen Deposition in <i>Stipa baicalensis</i> Steppe |
| title_full_unstemmed | The Roles of Bacteria in Soil Organic Carbon Accumulation under Nitrogen Deposition in <i>Stipa baicalensis</i> Steppe |
| title_short | The Roles of Bacteria in Soil Organic Carbon Accumulation under Nitrogen Deposition in <i>Stipa baicalensis</i> Steppe |
| title_sort | roles of bacteria in soil organic carbon accumulation under nitrogen deposition in i stipa baicalensis i steppe |
| topic | nitrogen deposition grassland soil organic carbon bacteria soil properties |
| url | https://www.mdpi.com/2076-2607/8/3/326 |
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