Production of pine sawdust biochar supporting phosphate-solubilizing bacteria as an alternative bioinoculant in Allium cepa L., culture
Abstract We produced and characterised biochar made from Caribbean pine sawdust as raw material. The biochar (BC500) was used as biocompatible support to co-inoculate phosphate solubilizing bacteria (PSB) (BC500/PSB) on Allium cepa L., plants at a greenhouse scale for four months. The three biomater...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-07-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-17106-1 |
| _version_ | 1828371546755825664 |
|---|---|
| author | Andrea Blanco-Vargas María A. Chacón-Buitrago María C. Quintero-Duque Raúl A. Poutou-Piñales Lucía A. Díaz-Ariza Carlos A. Devia-Castillo Laura C. Castillo-Carvajal Daniel Toledo-Aranda Christiano da Conceição de Matos Wilmar Olaya-González Oswaldo Ramos-Monroy Aura M. Pedroza-Rodríguez |
| author_facet | Andrea Blanco-Vargas María A. Chacón-Buitrago María C. Quintero-Duque Raúl A. Poutou-Piñales Lucía A. Díaz-Ariza Carlos A. Devia-Castillo Laura C. Castillo-Carvajal Daniel Toledo-Aranda Christiano da Conceição de Matos Wilmar Olaya-González Oswaldo Ramos-Monroy Aura M. Pedroza-Rodríguez |
| author_sort | Andrea Blanco-Vargas |
| collection | DOAJ |
| description | Abstract We produced and characterised biochar made from Caribbean pine sawdust as raw material. The biochar (BC500) was used as biocompatible support to co-inoculate phosphate solubilizing bacteria (PSB) (BC500/PSB) on Allium cepa L., plants at a greenhouse scale for four months. The three biomaterials study included proximate analysis, elemental analysis, aromaticity analysis, scanning electron microscopy, Fourier transform infrared spectroscopy (FTIR), adsorption studies at different pH and PSB stability as a function of time. The results indicated that BC500 is suitable as organic support or solid matrix to maintain the viability of PSB able to solubilise P from phosphate rock (PR). The biofertilizer (BC500/PSB) allows increasing germination, seedling growth, nutrient assimilation, and growth of Allium cepa L., because PSB immobilised on BC500 promoted nutrient mobilisation, particularly P, during cultivation of Allium cepa L., at pots scale. The two treatments to evaluate the biofertilizer (BC500/PSB) showed the highest concentrations of total P with 1.25 ± 0.13 and 1.38 ± 0.14 mg bulb−1 in A. cepa L. This work presents the benefits of a new product based on bacteria naturally associated with onion and an organic material (BC500) serving as a bacterial carrier that increases the adsorption area of highly reactive nutrients, reducing their leaching or precipitation with other nutrients and fixation to the solid matrix of the soil. |
| first_indexed | 2024-04-14T06:51:03Z |
| format | Article |
| id | doaj.art-0cc340c91293422a95a650b404bb8e1f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-14T06:51:03Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0cc340c91293422a95a650b404bb8e1f2022-12-22T02:07:02ZengNature PortfolioScientific Reports2045-23222022-07-0112111810.1038/s41598-022-17106-1Production of pine sawdust biochar supporting phosphate-solubilizing bacteria as an alternative bioinoculant in Allium cepa L., cultureAndrea Blanco-Vargas0María A. Chacón-Buitrago1María C. Quintero-Duque2Raúl A. Poutou-Piñales3Lucía A. Díaz-Ariza4Carlos A. Devia-Castillo5Laura C. Castillo-Carvajal6Daniel Toledo-Aranda7Christiano da Conceição de Matos8Wilmar Olaya-González9Oswaldo Ramos-Monroy10Aura M. Pedroza-Rodríguez11Laboratorio de Microbiología Ambiental y de Suelos, Unidad de Investigaciones Agropecuarias (UNIDIA), Departamento de Microbiología. Facultad de Ciencias, Pontificia Universidad JaverianaLaboratorio de Microbiología Ambiental y de Suelos, Unidad de Investigaciones Agropecuarias (UNIDIA), Departamento de Microbiología. Facultad de Ciencias, Pontificia Universidad JaverianaLaboratorio de Microbiología Ambiental y de Suelos, Unidad de Investigaciones Agropecuarias (UNIDIA), Departamento de Microbiología. Facultad de Ciencias, Pontificia Universidad JaverianaLaboratorio de Biotecnología Molecular, Grupo de Biotecnología Ambiental e Industrial (GBAI), Departamento de Microbiología. Facultad de Ciencias. Pontificia, Universidad JaverianaLaboratorio Asociaciones Suelo, Planta, Microorganismo (LAMIC). Grupo de Investigación en Agricultura Biológica, Departamento de Biología. Facultad de Ciencias. Pontificia, Universidad JaverianaFacultad de Estudios Ambientales y Rurales, Departamento de Ecología y Territorio. Pontificia, Universidad JaverianaFacultad de Ciencias de la Salud, Universidad Anáhuac Campus NorteFacultad de Ciencias de la Salud, Universidad Anáhuac Campus NorteDepartamento de Ciências Agrárias e Naturais, Universidade do Estado de Minas GeraisLaboratorio Asociaciones Suelo, Planta, Microorganismo (LAMIC). Grupo de Investigación en Agricultura Biológica, Departamento de Biología. Facultad de Ciencias. Pontificia, Universidad JaverianaInstituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prolongación de Carpio y Plan de Ayala S/N, Col. Santo TomásLaboratorio de Microbiología Ambiental y de Suelos, Unidad de Investigaciones Agropecuarias (UNIDIA), Departamento de Microbiología. Facultad de Ciencias, Pontificia Universidad JaverianaAbstract We produced and characterised biochar made from Caribbean pine sawdust as raw material. The biochar (BC500) was used as biocompatible support to co-inoculate phosphate solubilizing bacteria (PSB) (BC500/PSB) on Allium cepa L., plants at a greenhouse scale for four months. The three biomaterials study included proximate analysis, elemental analysis, aromaticity analysis, scanning electron microscopy, Fourier transform infrared spectroscopy (FTIR), adsorption studies at different pH and PSB stability as a function of time. The results indicated that BC500 is suitable as organic support or solid matrix to maintain the viability of PSB able to solubilise P from phosphate rock (PR). The biofertilizer (BC500/PSB) allows increasing germination, seedling growth, nutrient assimilation, and growth of Allium cepa L., because PSB immobilised on BC500 promoted nutrient mobilisation, particularly P, during cultivation of Allium cepa L., at pots scale. The two treatments to evaluate the biofertilizer (BC500/PSB) showed the highest concentrations of total P with 1.25 ± 0.13 and 1.38 ± 0.14 mg bulb−1 in A. cepa L. This work presents the benefits of a new product based on bacteria naturally associated with onion and an organic material (BC500) serving as a bacterial carrier that increases the adsorption area of highly reactive nutrients, reducing their leaching or precipitation with other nutrients and fixation to the solid matrix of the soil.https://doi.org/10.1038/s41598-022-17106-1 |
| spellingShingle | Andrea Blanco-Vargas María A. Chacón-Buitrago María C. Quintero-Duque Raúl A. Poutou-Piñales Lucía A. Díaz-Ariza Carlos A. Devia-Castillo Laura C. Castillo-Carvajal Daniel Toledo-Aranda Christiano da Conceição de Matos Wilmar Olaya-González Oswaldo Ramos-Monroy Aura M. Pedroza-Rodríguez Production of pine sawdust biochar supporting phosphate-solubilizing bacteria as an alternative bioinoculant in Allium cepa L., culture Scientific Reports |
| title | Production of pine sawdust biochar supporting phosphate-solubilizing bacteria as an alternative bioinoculant in Allium cepa L., culture |
| title_full | Production of pine sawdust biochar supporting phosphate-solubilizing bacteria as an alternative bioinoculant in Allium cepa L., culture |
| title_fullStr | Production of pine sawdust biochar supporting phosphate-solubilizing bacteria as an alternative bioinoculant in Allium cepa L., culture |
| title_full_unstemmed | Production of pine sawdust biochar supporting phosphate-solubilizing bacteria as an alternative bioinoculant in Allium cepa L., culture |
| title_short | Production of pine sawdust biochar supporting phosphate-solubilizing bacteria as an alternative bioinoculant in Allium cepa L., culture |
| title_sort | production of pine sawdust biochar supporting phosphate solubilizing bacteria as an alternative bioinoculant in allium cepa l culture |
| url | https://doi.org/10.1038/s41598-022-17106-1 |
| work_keys_str_mv | AT andreablancovargas productionofpinesawdustbiocharsupportingphosphatesolubilizingbacteriaasanalternativebioinoculantinalliumcepalculture AT mariaachaconbuitrago productionofpinesawdustbiocharsupportingphosphatesolubilizingbacteriaasanalternativebioinoculantinalliumcepalculture AT mariacquinteroduque productionofpinesawdustbiocharsupportingphosphatesolubilizingbacteriaasanalternativebioinoculantinalliumcepalculture AT raulapoutoupinales productionofpinesawdustbiocharsupportingphosphatesolubilizingbacteriaasanalternativebioinoculantinalliumcepalculture AT luciaadiazariza productionofpinesawdustbiocharsupportingphosphatesolubilizingbacteriaasanalternativebioinoculantinalliumcepalculture AT carlosadeviacastillo productionofpinesawdustbiocharsupportingphosphatesolubilizingbacteriaasanalternativebioinoculantinalliumcepalculture AT lauraccastillocarvajal productionofpinesawdustbiocharsupportingphosphatesolubilizingbacteriaasanalternativebioinoculantinalliumcepalculture AT danieltoledoaranda productionofpinesawdustbiocharsupportingphosphatesolubilizingbacteriaasanalternativebioinoculantinalliumcepalculture AT christianodaconceicaodematos productionofpinesawdustbiocharsupportingphosphatesolubilizingbacteriaasanalternativebioinoculantinalliumcepalculture AT wilmarolayagonzalez productionofpinesawdustbiocharsupportingphosphatesolubilizingbacteriaasanalternativebioinoculantinalliumcepalculture AT oswaldoramosmonroy productionofpinesawdustbiocharsupportingphosphatesolubilizingbacteriaasanalternativebioinoculantinalliumcepalculture AT aurampedrozarodriguez productionofpinesawdustbiocharsupportingphosphatesolubilizingbacteriaasanalternativebioinoculantinalliumcepalculture |