Biological analysis of an innovative biodegradable antibiotic eluting bioactive glass/gypsum composite bone cement for treating experimental chronic MRSA osteomyelitis

A multi-barrier antibiotics loaded biodegradable composite bone cement for resolving chronic osteomyelitis has been studied to understand the physico-mechanical properties, drug loading/eluting efficiency, and different merits and demerits prior to clinical application. After successful induction of...

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Main Authors: Surajit Mistry, Subhasish Burman, Subhasis Roy, Nilendu Jyoti Maitra, Rajiv Roy, Abhijit Chanda
Format: Article
Language:English
Published: Elsevier 2022-02-01
Series:Journal of Pharmaceutical Analysis
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2095177921000204
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author Surajit Mistry
Subhasish Burman
Subhasis Roy
Nilendu Jyoti Maitra
Rajiv Roy
Abhijit Chanda
author_facet Surajit Mistry
Subhasish Burman
Subhasis Roy
Nilendu Jyoti Maitra
Rajiv Roy
Abhijit Chanda
author_sort Surajit Mistry
collection DOAJ
description A multi-barrier antibiotics loaded biodegradable composite bone cement for resolving chronic osteomyelitis has been studied to understand the physico-mechanical properties, drug loading/eluting efficiency, and different merits and demerits prior to clinical application. After successful induction of bone infection in 28 rabbits using methicillin-resistant Staphylococcus aureus (MRSA) strains, calcium sulfate/bioactive glass based composite cement was implanted in 12 defects to assess its performance over parenteral therapy with microscopic and radiological examination for 90 days. The composite cement revealed acceptable physico-mechanical properties and controlled drug elution kinetics. Furthermore, the antibiotics concentrations in bone up to 42 days were sufficient to kill MRSA without eliciting adverse drug reactions. The striking feature of platelets aggregation by composite cement could assist bone healing. The controlled degradation with simultaneous entrapment of composite cement within the osteoid tissues and complete repair of infected cortical defects (holes) in rabbit tibia at 6 weeks indicated the excellent anti-infective and osteoconductive properties of composite cement. Thus, the animal study demonstrated the superiority of composite over injectable antibiotic therapy based on infection resolution and bone regeneration. We thereby conclude that the composite cement can be effectively applied in the treatment of resistant cases of chronic osteomyelitis.
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spelling doaj.art-0cc3decd7d1d4d668452bc06a90464582022-12-21T16:43:10ZengElsevierJournal of Pharmaceutical Analysis2095-17792022-02-01121164177Biological analysis of an innovative biodegradable antibiotic eluting bioactive glass/gypsum composite bone cement for treating experimental chronic MRSA osteomyelitisSurajit Mistry0Subhasish Burman1Subhasis Roy2Nilendu Jyoti Maitra3Rajiv Roy4Abhijit Chanda5Department of Periodontics, Burdwan Dental College & Hospital, Burdwan, 713101, India; Corresponding author.Department of Oral & Maxillofacial Surgery, Burdwan Dental College & Hospital, Burdwan, 713101, IndiaDepartment of Veterinary Clinical Complex (Surgery & Radiology), West Bengal University of Animal & Fishery Sciences, Kolkata, 700037, IndiaDirectorate of Research Extension & Farms, West Bengal University of Animal & Fishery Sciences, Kolkata, 700037, IndiaDepartment of Orthopaedics, Calcutta National Medical College & Hospital, Kolkata, 700014, IndiaSchool of Bio-Science & Engineering, Jadavpur University, Kolkata, 734432, IndiaA multi-barrier antibiotics loaded biodegradable composite bone cement for resolving chronic osteomyelitis has been studied to understand the physico-mechanical properties, drug loading/eluting efficiency, and different merits and demerits prior to clinical application. After successful induction of bone infection in 28 rabbits using methicillin-resistant Staphylococcus aureus (MRSA) strains, calcium sulfate/bioactive glass based composite cement was implanted in 12 defects to assess its performance over parenteral therapy with microscopic and radiological examination for 90 days. The composite cement revealed acceptable physico-mechanical properties and controlled drug elution kinetics. Furthermore, the antibiotics concentrations in bone up to 42 days were sufficient to kill MRSA without eliciting adverse drug reactions. The striking feature of platelets aggregation by composite cement could assist bone healing. The controlled degradation with simultaneous entrapment of composite cement within the osteoid tissues and complete repair of infected cortical defects (holes) in rabbit tibia at 6 weeks indicated the excellent anti-infective and osteoconductive properties of composite cement. Thus, the animal study demonstrated the superiority of composite over injectable antibiotic therapy based on infection resolution and bone regeneration. We thereby conclude that the composite cement can be effectively applied in the treatment of resistant cases of chronic osteomyelitis.http://www.sciencedirect.com/science/article/pii/S2095177921000204AntibioticsBiodegradable compositeOsteomyelitisMRSA strainsOsteoconduction
spellingShingle Surajit Mistry
Subhasish Burman
Subhasis Roy
Nilendu Jyoti Maitra
Rajiv Roy
Abhijit Chanda
Biological analysis of an innovative biodegradable antibiotic eluting bioactive glass/gypsum composite bone cement for treating experimental chronic MRSA osteomyelitis
Journal of Pharmaceutical Analysis
Antibiotics
Biodegradable composite
Osteomyelitis
MRSA strains
Osteoconduction
title Biological analysis of an innovative biodegradable antibiotic eluting bioactive glass/gypsum composite bone cement for treating experimental chronic MRSA osteomyelitis
title_full Biological analysis of an innovative biodegradable antibiotic eluting bioactive glass/gypsum composite bone cement for treating experimental chronic MRSA osteomyelitis
title_fullStr Biological analysis of an innovative biodegradable antibiotic eluting bioactive glass/gypsum composite bone cement for treating experimental chronic MRSA osteomyelitis
title_full_unstemmed Biological analysis of an innovative biodegradable antibiotic eluting bioactive glass/gypsum composite bone cement for treating experimental chronic MRSA osteomyelitis
title_short Biological analysis of an innovative biodegradable antibiotic eluting bioactive glass/gypsum composite bone cement for treating experimental chronic MRSA osteomyelitis
title_sort biological analysis of an innovative biodegradable antibiotic eluting bioactive glass gypsum composite bone cement for treating experimental chronic mrsa osteomyelitis
topic Antibiotics
Biodegradable composite
Osteomyelitis
MRSA strains
Osteoconduction
url http://www.sciencedirect.com/science/article/pii/S2095177921000204
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