Steinernema feltiae- Xenorhabdus bovienii: more information on this bactohelminthic complex from Iran
Two families of entomopathogenic nematodes (EPNs), Steinernematidae and Heterorhabditidae, that are symbiotically associated with Xenorhabdus and Photorhabdus bacteria, are one of the effective biological control agents of insect pests. Native isolates can probably be more efficacious to control ins...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Ferdowsi University of Mashhad
2022-12-01
|
| Series: | Iranian Journal of Animal Biosystematics |
| Subjects: | |
| Online Access: | https://ijab.um.ac.ir/article_43107_a8f17d668502db6d40968046c0c1e33c.pdf |
| _version_ | 1827378165506375680 |
|---|---|
| author | Kimia Kuhestani Javad Karimi Ali Makhdoumi |
| author_facet | Kimia Kuhestani Javad Karimi Ali Makhdoumi |
| author_sort | Kimia Kuhestani |
| collection | DOAJ |
| description | Two families of entomopathogenic nematodes (EPNs), Steinernematidae and Heterorhabditidae, that are symbiotically associated with Xenorhabdus and Photorhabdus bacteria, are one of the effective biological control agents of insect pests. Native isolates can probably be more efficacious to control insect pests than exotic ones due to their adaptability to indigenous environmental conditions. In this study, Steinernema feltiae isolate FUM221, was recovered from soil samples collected from the Ardabil Province, Iran. Morphological and morphometric investigations of the first and second-generation adults, infective juveniles, and molecular characterizations were given based on ITS and 18S rDNA genes. Besides, molecular analysis based on the 16S rRNA region and phenetic data recognized Xenorhabdus bovienii as its symbiont bacterium. The scanning electron microscopy (SEM) images verified the identification of this isolate. The molecular characterization using two loci and phylogenetic analyses provided more evidence for the classification of this steinernematid and its difference of the same species from other countries. Moreover, molecular and phenetic characterizations of its symbiotic bacterium were provided with low variations compared to other isolates. Herein, the comprehensive taxonomic data of this steinernematid and its symbiont bacterium, is presented from Iran. |
| first_indexed | 2024-03-08T12:51:04Z |
| format | Article |
| id | doaj.art-0cc5af78b97442f6bc9f118e0537dcbe |
| institution | Directory Open Access Journal |
| issn | 1735-434X 2423-4222 |
| language | English |
| last_indexed | 2024-03-08T12:51:04Z |
| publishDate | 2022-12-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | Iranian Journal of Animal Biosystematics |
| spelling | doaj.art-0cc5af78b97442f6bc9f118e0537dcbe2024-01-20T05:29:03ZengFerdowsi University of MashhadIranian Journal of Animal Biosystematics1735-434X2423-42222022-12-0118213916110.22067/ijab.2022.78592.103843107Steinernema feltiae- Xenorhabdus bovienii: more information on this bactohelminthic complex from IranKimia Kuhestani0Javad Karimi1Ali Makhdoumi2Department of Plant Protection, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Plant Protection, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Biology, Ferdowsi University of Mashhad, IranTwo families of entomopathogenic nematodes (EPNs), Steinernematidae and Heterorhabditidae, that are symbiotically associated with Xenorhabdus and Photorhabdus bacteria, are one of the effective biological control agents of insect pests. Native isolates can probably be more efficacious to control insect pests than exotic ones due to their adaptability to indigenous environmental conditions. In this study, Steinernema feltiae isolate FUM221, was recovered from soil samples collected from the Ardabil Province, Iran. Morphological and morphometric investigations of the first and second-generation adults, infective juveniles, and molecular characterizations were given based on ITS and 18S rDNA genes. Besides, molecular analysis based on the 16S rRNA region and phenetic data recognized Xenorhabdus bovienii as its symbiont bacterium. The scanning electron microscopy (SEM) images verified the identification of this isolate. The molecular characterization using two loci and phylogenetic analyses provided more evidence for the classification of this steinernematid and its difference of the same species from other countries. Moreover, molecular and phenetic characterizations of its symbiotic bacterium were provided with low variations compared to other isolates. Herein, the comprehensive taxonomic data of this steinernematid and its symbiont bacterium, is presented from Iran.https://ijab.um.ac.ir/article_43107_a8f17d668502db6d40968046c0c1e33c.pdfcharacterizationentomopathogenic nematodessteinernemasurveytaxon, xenorhabdus |
| spellingShingle | Kimia Kuhestani Javad Karimi Ali Makhdoumi Steinernema feltiae- Xenorhabdus bovienii: more information on this bactohelminthic complex from Iran Iranian Journal of Animal Biosystematics characterization entomopathogenic nematodes steinernema survey taxon, xenorhabdus |
| title | Steinernema feltiae- Xenorhabdus bovienii: more information on this bactohelminthic complex from Iran |
| title_full | Steinernema feltiae- Xenorhabdus bovienii: more information on this bactohelminthic complex from Iran |
| title_fullStr | Steinernema feltiae- Xenorhabdus bovienii: more information on this bactohelminthic complex from Iran |
| title_full_unstemmed | Steinernema feltiae- Xenorhabdus bovienii: more information on this bactohelminthic complex from Iran |
| title_short | Steinernema feltiae- Xenorhabdus bovienii: more information on this bactohelminthic complex from Iran |
| title_sort | steinernema feltiae xenorhabdus bovienii more information on this bactohelminthic complex from iran |
| topic | characterization entomopathogenic nematodes steinernema survey taxon, xenorhabdus |
| url | https://ijab.um.ac.ir/article_43107_a8f17d668502db6d40968046c0c1e33c.pdf |
| work_keys_str_mv | AT kimiakuhestani steinernemafeltiaexenorhabdusbovieniimoreinformationonthisbactohelminthiccomplexfromiran AT javadkarimi steinernemafeltiaexenorhabdusbovieniimoreinformationonthisbactohelminthiccomplexfromiran AT alimakhdoumi steinernemafeltiaexenorhabdusbovieniimoreinformationonthisbactohelminthiccomplexfromiran |