Rescuing ocular development in an anophthalmic pig by blastocyst complementation
Abstract Porcine‐derived xenogeneic sources for transplantation are a promising alternative strategy for providing organs for treatment of end‐stage organ failure in human patients because of the shortage of human donor organs. The recently developed blastocyst or pluripotent stem cell (PSC) complem...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Nature
2018-12-01
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| Series: | EMBO Molecular Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.15252/emmm.201808861 |
| _version_ | 1797283215027732480 |
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| author | Hongyong Zhang Jiaojiao Huang Zechen Li Guosong Qin Nan Zhang Tang Hai Qianlong Hong Qiantao Zheng Ying Zhang Ruigao Song Jing Yao Chunwei Cao Jianguo Zhao Qi Zhou |
| author_facet | Hongyong Zhang Jiaojiao Huang Zechen Li Guosong Qin Nan Zhang Tang Hai Qianlong Hong Qiantao Zheng Ying Zhang Ruigao Song Jing Yao Chunwei Cao Jianguo Zhao Qi Zhou |
| author_sort | Hongyong Zhang |
| collection | DOAJ |
| description | Abstract Porcine‐derived xenogeneic sources for transplantation are a promising alternative strategy for providing organs for treatment of end‐stage organ failure in human patients because of the shortage of human donor organs. The recently developed blastocyst or pluripotent stem cell (PSC) complementation strategy opens a new route for regenerating allogenic organs in miniature pigs. Since the eye is a complicated organ with highly specialized constituent tissues derived from different primordial cell lineages, the development of an intact eye from allogenic cells is a challenging task. Here, combining somatic cell nuclear transfer technology (SCNT) and an anophthalmic pig model (MITFL247S/L247S), allogenic retinal pigmented epithelium cells (RPEs) were retrieved from an E60 chimeric fetus using blastocyst complementation. Furthermore, all structures were successfully regenerated in the intact eye from the injected donor blastomeres. These results clearly demonstrate that not only differentiated functional somatic cells but also a disabled organ with highly specialized constituent tissues can be generated from exogenous blastomeres when delivered to pig embryos with an empty organ niche. This system may also provide novel insights into ocular organogenesis. |
| first_indexed | 2024-03-07T17:28:47Z |
| format | Article |
| id | doaj.art-0cc8f0970f30466584f98fde466fdfb2 |
| institution | Directory Open Access Journal |
| issn | 1757-4676 1757-4684 |
| language | English |
| last_indexed | 2024-03-07T17:28:47Z |
| publishDate | 2018-12-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | EMBO Molecular Medicine |
| spelling | doaj.art-0cc8f0970f30466584f98fde466fdfb22024-03-02T18:30:27ZengSpringer NatureEMBO Molecular Medicine1757-46761757-46842018-12-011012n/an/a10.15252/emmm.201808861Rescuing ocular development in an anophthalmic pig by blastocyst complementationHongyong Zhang0Jiaojiao Huang1Zechen Li2Guosong Qin3Nan Zhang4Tang Hai5Qianlong Hong6Qiantao Zheng7Ying Zhang8Ruigao Song9Jing Yao10Chunwei Cao11Jianguo Zhao12Qi Zhou13State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing ChinaCollege of Life Sciences Qufu Normal University Qufu ChinaState Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing ChinaAbstract Porcine‐derived xenogeneic sources for transplantation are a promising alternative strategy for providing organs for treatment of end‐stage organ failure in human patients because of the shortage of human donor organs. The recently developed blastocyst or pluripotent stem cell (PSC) complementation strategy opens a new route for regenerating allogenic organs in miniature pigs. Since the eye is a complicated organ with highly specialized constituent tissues derived from different primordial cell lineages, the development of an intact eye from allogenic cells is a challenging task. Here, combining somatic cell nuclear transfer technology (SCNT) and an anophthalmic pig model (MITFL247S/L247S), allogenic retinal pigmented epithelium cells (RPEs) were retrieved from an E60 chimeric fetus using blastocyst complementation. Furthermore, all structures were successfully regenerated in the intact eye from the injected donor blastomeres. These results clearly demonstrate that not only differentiated functional somatic cells but also a disabled organ with highly specialized constituent tissues can be generated from exogenous blastomeres when delivered to pig embryos with an empty organ niche. This system may also provide novel insights into ocular organogenesis.https://doi.org/10.15252/emmm.201808861anophthalmic pigchimeraorgan regenerationsomatic cell nuclear transfer technology |
| spellingShingle | Hongyong Zhang Jiaojiao Huang Zechen Li Guosong Qin Nan Zhang Tang Hai Qianlong Hong Qiantao Zheng Ying Zhang Ruigao Song Jing Yao Chunwei Cao Jianguo Zhao Qi Zhou Rescuing ocular development in an anophthalmic pig by blastocyst complementation EMBO Molecular Medicine anophthalmic pig chimera organ regeneration somatic cell nuclear transfer technology |
| title | Rescuing ocular development in an anophthalmic pig by blastocyst complementation |
| title_full | Rescuing ocular development in an anophthalmic pig by blastocyst complementation |
| title_fullStr | Rescuing ocular development in an anophthalmic pig by blastocyst complementation |
| title_full_unstemmed | Rescuing ocular development in an anophthalmic pig by blastocyst complementation |
| title_short | Rescuing ocular development in an anophthalmic pig by blastocyst complementation |
| title_sort | rescuing ocular development in an anophthalmic pig by blastocyst complementation |
| topic | anophthalmic pig chimera organ regeneration somatic cell nuclear transfer technology |
| url | https://doi.org/10.15252/emmm.201808861 |
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