“Green” Extraction and On-Site Rapid Detection of Aflatoxin B1, Zearalenone and Deoxynivalenol in Corn, Rice and Peanut
The common mycotoxins in polluted grains are aflatoxin B1(AFB1), zearalenone (ZEN) and deoxynivalenol (DON). Because of the potential threat to humans and animals, it is necessary to detect mycotoxin contaminants rapidly. At present, later flow immunoassay (LFIA) is one of the most frequently used m...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/7/3260 |
| _version_ | 1797607314205704192 |
|---|---|
| author | Zijing Li Zepeng Li Xintong Li Qi Fan Yinuo Chen Guoqing Shi |
| author_facet | Zijing Li Zepeng Li Xintong Li Qi Fan Yinuo Chen Guoqing Shi |
| author_sort | Zijing Li |
| collection | DOAJ |
| description | The common mycotoxins in polluted grains are aflatoxin B1(AFB1), zearalenone (ZEN) and deoxynivalenol (DON). Because of the potential threat to humans and animals, it is necessary to detect mycotoxin contaminants rapidly. At present, later flow immunoassay (LFIA) is one of the most frequently used methods for rapid analysis. However, multistep sample pretreatment processes and organic solvents are also required to extract mycotoxins from grains. In this study, we developed a one-step and “green” sample pretreatment method without using organic solvents. By combining with LFIA test strips and a handheld detection device, an on-site method for the rapid detection of AFB1, ZEN and DON was developed. The LODs for AFB1, ZEN and DON in corn are 0.90 μg/kg, 7.11 μg/kg and 10.6 μg/kg, respectively, and the working ranges are from 1.25 μg/kg to 40 μg/kg, 20 μg/kg to 2000 μg/kg and 35 μg/kg to 1500 μg/kg, respectively. This method has been successfully applied to the detection of AFB1, ZEN and DON in corn, rice and peanut, with recoveries of 89 ± 3%–106 ± 3%, 86 ± 2%–108 ± 7% and 90 ± 2%–106 ± 10%, respectively. The detection results for the AFB1, ZEN and DON residues in certified reference materials by this method were in good agreement with their certificate values. |
| first_indexed | 2024-03-11T05:28:23Z |
| format | Article |
| id | doaj.art-0ccdbe66132b4d75983ffb02940fd03b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T05:28:23Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0ccdbe66132b4d75983ffb02940fd03b2023-11-17T17:15:54ZengMDPI AGMolecules1420-30492023-04-01287326010.3390/molecules28073260“Green” Extraction and On-Site Rapid Detection of Aflatoxin B1, Zearalenone and Deoxynivalenol in Corn, Rice and PeanutZijing Li0Zepeng Li1Xintong Li2Qi Fan3Yinuo Chen4Guoqing Shi5School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, ChinaSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, ChinaSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, ChinaSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, ChinaSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, ChinaSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, ChinaThe common mycotoxins in polluted grains are aflatoxin B1(AFB1), zearalenone (ZEN) and deoxynivalenol (DON). Because of the potential threat to humans and animals, it is necessary to detect mycotoxin contaminants rapidly. At present, later flow immunoassay (LFIA) is one of the most frequently used methods for rapid analysis. However, multistep sample pretreatment processes and organic solvents are also required to extract mycotoxins from grains. In this study, we developed a one-step and “green” sample pretreatment method without using organic solvents. By combining with LFIA test strips and a handheld detection device, an on-site method for the rapid detection of AFB1, ZEN and DON was developed. The LODs for AFB1, ZEN and DON in corn are 0.90 μg/kg, 7.11 μg/kg and 10.6 μg/kg, respectively, and the working ranges are from 1.25 μg/kg to 40 μg/kg, 20 μg/kg to 2000 μg/kg and 35 μg/kg to 1500 μg/kg, respectively. This method has been successfully applied to the detection of AFB1, ZEN and DON in corn, rice and peanut, with recoveries of 89 ± 3%–106 ± 3%, 86 ± 2%–108 ± 7% and 90 ± 2%–106 ± 10%, respectively. The detection results for the AFB1, ZEN and DON residues in certified reference materials by this method were in good agreement with their certificate values.https://www.mdpi.com/1420-3049/28/7/3260lateral flow immunoassayaflatoxin B1zearalenonedeoxynivalenolrapid detection |
| spellingShingle | Zijing Li Zepeng Li Xintong Li Qi Fan Yinuo Chen Guoqing Shi “Green” Extraction and On-Site Rapid Detection of Aflatoxin B1, Zearalenone and Deoxynivalenol in Corn, Rice and Peanut Molecules lateral flow immunoassay aflatoxin B1 zearalenone deoxynivalenol rapid detection |
| title | “Green” Extraction and On-Site Rapid Detection of Aflatoxin B1, Zearalenone and Deoxynivalenol in Corn, Rice and Peanut |
| title_full | “Green” Extraction and On-Site Rapid Detection of Aflatoxin B1, Zearalenone and Deoxynivalenol in Corn, Rice and Peanut |
| title_fullStr | “Green” Extraction and On-Site Rapid Detection of Aflatoxin B1, Zearalenone and Deoxynivalenol in Corn, Rice and Peanut |
| title_full_unstemmed | “Green” Extraction and On-Site Rapid Detection of Aflatoxin B1, Zearalenone and Deoxynivalenol in Corn, Rice and Peanut |
| title_short | “Green” Extraction and On-Site Rapid Detection of Aflatoxin B1, Zearalenone and Deoxynivalenol in Corn, Rice and Peanut |
| title_sort | green extraction and on site rapid detection of aflatoxin b1 zearalenone and deoxynivalenol in corn rice and peanut |
| topic | lateral flow immunoassay aflatoxin B1 zearalenone deoxynivalenol rapid detection |
| url | https://www.mdpi.com/1420-3049/28/7/3260 |
| work_keys_str_mv | AT zijingli greenextractionandonsiterapiddetectionofaflatoxinb1zearalenoneanddeoxynivalenolincornriceandpeanut AT zepengli greenextractionandonsiterapiddetectionofaflatoxinb1zearalenoneanddeoxynivalenolincornriceandpeanut AT xintongli greenextractionandonsiterapiddetectionofaflatoxinb1zearalenoneanddeoxynivalenolincornriceandpeanut AT qifan greenextractionandonsiterapiddetectionofaflatoxinb1zearalenoneanddeoxynivalenolincornriceandpeanut AT yinuochen greenextractionandonsiterapiddetectionofaflatoxinb1zearalenoneanddeoxynivalenolincornriceandpeanut AT guoqingshi greenextractionandonsiterapiddetectionofaflatoxinb1zearalenoneanddeoxynivalenolincornriceandpeanut |