Photobiomodulation effects of pulsed-NIR laser (810 nm) and LED (808 ± 3 nm) with identical treatment regimen on burn wound healing: A quantitative label-free global proteomic approach
Photobiomodulation (PBM) has evolved as a rapidly growing therapeutic biophysical non-invasive approach for the acceleration of tissue repair, mitigation of pain, inflammation and restoration of cellular functions. This study compares the PBM effect of pulsed-mode (10 Hz) of NIR laser (810 nm) and L...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Journal of Photochemistry and Photobiology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666469021000099 |
| _version_ | 1818665105517182976 |
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| author | Gaurav K. Keshri Gaurav Kumar Manish Sharma Kiran Bora Bhuvnesh Kumar Asheesh Gupta |
| author_facet | Gaurav K. Keshri Gaurav Kumar Manish Sharma Kiran Bora Bhuvnesh Kumar Asheesh Gupta |
| author_sort | Gaurav K. Keshri |
| collection | DOAJ |
| description | Photobiomodulation (PBM) has evolved as a rapidly growing therapeutic biophysical non-invasive approach for the acceleration of tissue repair, mitigation of pain, inflammation and restoration of cellular functions. This study compares the PBM effect of pulsed-mode (10 Hz) of NIR laser (810 nm) and LED (808 ± 3 nm) with identical treatment regimen (average power 70 mW; average irradiance 40 mW/cm2; total fluence 24 J/cm2, duty cycle 50%; pulse duration 50 msec; peak irradiance 80 mW/cm2; 10 min exposure once daily for 7 days) on full-thickness, third-degree burn wound in rat using comprehensive analysis of quantitative label-free global proteomics, followed by validation of the proteomics data by various biophysical, biochemical, molecular, histological and immunohistochemical (IHC) assays. The proteomic analysis clearly revealed the common biological processes indicated by modulation of similar biological pathways (known for tissue repair process) associated with neuronal (4), metabolic (10), vascular (3), inflammation (4) and cell signaling (12) in both laser and LED treated groups. Validation of proteomic analysis using various healing markers demonstrated attenuated inflammatory response like decreased TNF-α, IL-1β, IL-6, COX-2 levels (ELISA), enhanced cellular proliferation (PCNA, TGF-β2), collagen, ECM accumulation (biochemical, H&E, Masson's trichrome staining, IHC assays), wound contraction and cytoprotection (TUNEL assay) in both laser and LED-treated groups as compared to the control. Collectively, the proteomics data revealed previously know molecules along with novel identified molecules post-PBM treatment, which broaden the understanding of tissue repair mechanisms. This study profoundly signifies that both laser and LED in 810 nm wavelength range at pulsed-mode (10 Hz) are equally effective for PBM-mediated potential treatment to accelerate burn wound healing. |
| first_indexed | 2024-12-17T05:43:21Z |
| format | Article |
| id | doaj.art-0cd2b585ee8e4c7c9184c52c33365b0f |
| institution | Directory Open Access Journal |
| issn | 2666-4690 |
| language | English |
| last_indexed | 2024-12-17T05:43:21Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Photochemistry and Photobiology |
| spelling | doaj.art-0cd2b585ee8e4c7c9184c52c33365b0f2022-12-21T22:01:23ZengElsevierJournal of Photochemistry and Photobiology2666-46902021-06-016100024Photobiomodulation effects of pulsed-NIR laser (810 nm) and LED (808 ± 3 nm) with identical treatment regimen on burn wound healing: A quantitative label-free global proteomic approachGaurav K. Keshri0Gaurav Kumar1Manish Sharma2Kiran Bora3Bhuvnesh Kumar4Asheesh Gupta5Defence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Delhi 110054, IndiaDefence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Delhi 110054, IndiaDefence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Delhi 110054, IndiaDefence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Delhi 110054, IndiaDefence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Delhi 110054, IndiaCorresponding author.; Defence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Delhi 110054, IndiaPhotobiomodulation (PBM) has evolved as a rapidly growing therapeutic biophysical non-invasive approach for the acceleration of tissue repair, mitigation of pain, inflammation and restoration of cellular functions. This study compares the PBM effect of pulsed-mode (10 Hz) of NIR laser (810 nm) and LED (808 ± 3 nm) with identical treatment regimen (average power 70 mW; average irradiance 40 mW/cm2; total fluence 24 J/cm2, duty cycle 50%; pulse duration 50 msec; peak irradiance 80 mW/cm2; 10 min exposure once daily for 7 days) on full-thickness, third-degree burn wound in rat using comprehensive analysis of quantitative label-free global proteomics, followed by validation of the proteomics data by various biophysical, biochemical, molecular, histological and immunohistochemical (IHC) assays. The proteomic analysis clearly revealed the common biological processes indicated by modulation of similar biological pathways (known for tissue repair process) associated with neuronal (4), metabolic (10), vascular (3), inflammation (4) and cell signaling (12) in both laser and LED treated groups. Validation of proteomic analysis using various healing markers demonstrated attenuated inflammatory response like decreased TNF-α, IL-1β, IL-6, COX-2 levels (ELISA), enhanced cellular proliferation (PCNA, TGF-β2), collagen, ECM accumulation (biochemical, H&E, Masson's trichrome staining, IHC assays), wound contraction and cytoprotection (TUNEL assay) in both laser and LED-treated groups as compared to the control. Collectively, the proteomics data revealed previously know molecules along with novel identified molecules post-PBM treatment, which broaden the understanding of tissue repair mechanisms. This study profoundly signifies that both laser and LED in 810 nm wavelength range at pulsed-mode (10 Hz) are equally effective for PBM-mediated potential treatment to accelerate burn wound healing.http://www.sciencedirect.com/science/article/pii/S2666469021000099BurnDermalLaserLabel-free proteomicsLEDPhotobiomodulation |
| spellingShingle | Gaurav K. Keshri Gaurav Kumar Manish Sharma Kiran Bora Bhuvnesh Kumar Asheesh Gupta Photobiomodulation effects of pulsed-NIR laser (810 nm) and LED (808 ± 3 nm) with identical treatment regimen on burn wound healing: A quantitative label-free global proteomic approach Journal of Photochemistry and Photobiology Burn Dermal Laser Label-free proteomics LED Photobiomodulation |
| title | Photobiomodulation effects of pulsed-NIR laser (810 nm) and LED (808 ± 3 nm) with identical treatment regimen on burn wound healing: A quantitative label-free global proteomic approach |
| title_full | Photobiomodulation effects of pulsed-NIR laser (810 nm) and LED (808 ± 3 nm) with identical treatment regimen on burn wound healing: A quantitative label-free global proteomic approach |
| title_fullStr | Photobiomodulation effects of pulsed-NIR laser (810 nm) and LED (808 ± 3 nm) with identical treatment regimen on burn wound healing: A quantitative label-free global proteomic approach |
| title_full_unstemmed | Photobiomodulation effects of pulsed-NIR laser (810 nm) and LED (808 ± 3 nm) with identical treatment regimen on burn wound healing: A quantitative label-free global proteomic approach |
| title_short | Photobiomodulation effects of pulsed-NIR laser (810 nm) and LED (808 ± 3 nm) with identical treatment regimen on burn wound healing: A quantitative label-free global proteomic approach |
| title_sort | photobiomodulation effects of pulsed nir laser 810 nm and led 808 3 nm with identical treatment regimen on burn wound healing a quantitative label free global proteomic approach |
| topic | Burn Dermal Laser Label-free proteomics LED Photobiomodulation |
| url | http://www.sciencedirect.com/science/article/pii/S2666469021000099 |
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