Changes of biochemical parameters in rat intestinal mucosa induced by methotrexate and effects of enteral administration of glutamine
BACKGROUND: Rapidly proliferating crypt cells of the intestinal epithelium, the precursors of the mature enterocytes, are extremely sensitive to the effects of cytostatic agents. We investigated the effects of the methotrexate on rat intestinal mucosa in order to get the information on biochemical i...
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| Format: | Article |
| Language: | English |
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Institute of Oncology, Sremska Kamenica, Serbia
2004-01-01
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| Series: | Archive of Oncology |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-7310/2004/0354-73100401035B.pdf |
| _version_ | 1818964020197064704 |
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| author | Bajin-Katić Katica Stankov Karmen Kovačević Zoran |
| author_facet | Bajin-Katić Katica Stankov Karmen Kovačević Zoran |
| author_sort | Bajin-Katić Katica |
| collection | DOAJ |
| description | BACKGROUND: Rapidly proliferating crypt cells of the intestinal epithelium, the precursors of the mature enterocytes, are extremely sensitive to the effects of cytostatic agents. We investigated the effects of the methotrexate on rat intestinal mucosa in order to get the information on biochemical indicators of intestinal damage. METHODS: Biochemical parameters were investigated in isolated intestinal mucosa of Sprague-Dawley rats, previously treated with methotrexate by intraperitoneal administration. Glutamine was dissolved in water and administered orally. RESULTS: The activity of glutaminase and alkaline phosphatase showed the enzymatic response to different doses of methotrexate. The activity of both enzymes was significantly lower in the mucosa of treated animals, compared to control group. CONCLUSION: Minimal mucosal damage and regeneration time is dose dependent and influenced by the dosage schedule of antitumor therapy. |
| first_indexed | 2024-12-20T12:54:28Z |
| format | Article |
| id | doaj.art-0cd47946d49a4bc28e3de11c700d1ed5 |
| institution | Directory Open Access Journal |
| issn | 0354-7310 |
| language | English |
| last_indexed | 2024-12-20T12:54:28Z |
| publishDate | 2004-01-01 |
| publisher | Institute of Oncology, Sremska Kamenica, Serbia |
| record_format | Article |
| series | Archive of Oncology |
| spelling | doaj.art-0cd47946d49a4bc28e3de11c700d1ed52022-12-21T19:40:06ZengInstitute of Oncology, Sremska Kamenica, SerbiaArchive of Oncology0354-73102004-01-01121353810.2298/AOO0401035BChanges of biochemical parameters in rat intestinal mucosa induced by methotrexate and effects of enteral administration of glutamineBajin-Katić KaticaStankov KarmenKovačević ZoranBACKGROUND: Rapidly proliferating crypt cells of the intestinal epithelium, the precursors of the mature enterocytes, are extremely sensitive to the effects of cytostatic agents. We investigated the effects of the methotrexate on rat intestinal mucosa in order to get the information on biochemical indicators of intestinal damage. METHODS: Biochemical parameters were investigated in isolated intestinal mucosa of Sprague-Dawley rats, previously treated with methotrexate by intraperitoneal administration. Glutamine was dissolved in water and administered orally. RESULTS: The activity of glutaminase and alkaline phosphatase showed the enzymatic response to different doses of methotrexate. The activity of both enzymes was significantly lower in the mucosa of treated animals, compared to control group. CONCLUSION: Minimal mucosal damage and regeneration time is dose dependent and influenced by the dosage schedule of antitumor therapy.http://www.doiserbia.nb.rs/img/doi/0354-7310/2004/0354-73100401035B.pdfmethotrexateglutamineintestinal mucosaratsdrug toxicity |
| spellingShingle | Bajin-Katić Katica Stankov Karmen Kovačević Zoran Changes of biochemical parameters in rat intestinal mucosa induced by methotrexate and effects of enteral administration of glutamine Archive of Oncology methotrexate glutamine intestinal mucosa rats drug toxicity |
| title | Changes of biochemical parameters in rat intestinal mucosa induced by methotrexate and effects of enteral administration of glutamine |
| title_full | Changes of biochemical parameters in rat intestinal mucosa induced by methotrexate and effects of enteral administration of glutamine |
| title_fullStr | Changes of biochemical parameters in rat intestinal mucosa induced by methotrexate and effects of enteral administration of glutamine |
| title_full_unstemmed | Changes of biochemical parameters in rat intestinal mucosa induced by methotrexate and effects of enteral administration of glutamine |
| title_short | Changes of biochemical parameters in rat intestinal mucosa induced by methotrexate and effects of enteral administration of glutamine |
| title_sort | changes of biochemical parameters in rat intestinal mucosa induced by methotrexate and effects of enteral administration of glutamine |
| topic | methotrexate glutamine intestinal mucosa rats drug toxicity |
| url | http://www.doiserbia.nb.rs/img/doi/0354-7310/2004/0354-73100401035B.pdf |
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