Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell Encapsulation
The three-dimensional complexity of the native extracellular matrix (ECM) suggests switching from 2D to 3D culture systems for providing the cells with an architecture more similar to the physiological environment. Reproducing the three-dimensionality in vitro can guarantee beneficial effects in ter...
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MDPI AG
2020-09-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/18/6550 |
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| author | Antonella Stanzione Alessandro Polini Velia La Pesa Alessandro Romano Angelo Quattrini Giuseppe Gigli Lorenzo Moroni Francesca Gervaso |
| author_facet | Antonella Stanzione Alessandro Polini Velia La Pesa Alessandro Romano Angelo Quattrini Giuseppe Gigli Lorenzo Moroni Francesca Gervaso |
| author_sort | Antonella Stanzione |
| collection | DOAJ |
| description | The three-dimensional complexity of the native extracellular matrix (ECM) suggests switching from 2D to 3D culture systems for providing the cells with an architecture more similar to the physiological environment. Reproducing the three-dimensionality in vitro can guarantee beneficial effects in terms of cell growth, adhesion, proliferation, and/or their differentiation. Hydrogels have the same tailorable physico-chemical and biological characteristics as ECM materials. In this study, we propose a thermoresponsive chitosan-based hydrogel that gels thanks to the addition of organic and inorganic salt solutions (beta-glycerolphosphate and sodium hydrogen carbonate) and is suitable for cell encapsulation allowing obtaining 3D culture systems. Physico-chemical analyses showed that the hydrogel formulations jellify at physiological conditions (37 °C, pH 7.4), are stable in vitro up to three weeks, have high swelling ratios and mechanical stiffness suitable for cellular encapsulation. Moreover, preliminary biological tests underlined the pronounced biocompatibility of the system. Therefore, these chitosan-based hydrogels are proposed as valid biomaterials for cell encapsulation. |
| first_indexed | 2024-03-10T16:12:15Z |
| format | Article |
| id | doaj.art-0cd930d02812420f9ba2a75fb8dc4c3f |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T16:12:15Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-0cd930d02812420f9ba2a75fb8dc4c3f2023-11-20T14:21:15ZengMDPI AGApplied Sciences2076-34172020-09-011018655010.3390/app10186550Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell EncapsulationAntonella Stanzione0Alessandro Polini1Velia La Pesa2Alessandro Romano3Angelo Quattrini4Giuseppe Gigli5Lorenzo Moroni6Francesca Gervaso7Dipartimento di Matematica e Fisica E. De Giorgi, University of Salento, 73100 Lecce LE, ItalyCNR-Nanotec, Institute of Nanotechnology, 73100 Lecce, ItalyIRCCS San Raffaele Scientific Institute, Neuropathology Unit, Institute of Experimental Neurology and Division of Neuroscience, 20132 Milan, ItalyIRCCS San Raffaele Scientific Institute, Neuropathology Unit, Institute of Experimental Neurology and Division of Neuroscience, 20132 Milan, ItalyIRCCS San Raffaele Scientific Institute, Neuropathology Unit, Institute of Experimental Neurology and Division of Neuroscience, 20132 Milan, ItalyDipartimento di Matematica e Fisica E. De Giorgi, University of Salento, 73100 Lecce LE, ItalyCNR-Nanotec, Institute of Nanotechnology, 73100 Lecce, ItalyCNR-Nanotec, Institute of Nanotechnology, 73100 Lecce, ItalyThe three-dimensional complexity of the native extracellular matrix (ECM) suggests switching from 2D to 3D culture systems for providing the cells with an architecture more similar to the physiological environment. Reproducing the three-dimensionality in vitro can guarantee beneficial effects in terms of cell growth, adhesion, proliferation, and/or their differentiation. Hydrogels have the same tailorable physico-chemical and biological characteristics as ECM materials. In this study, we propose a thermoresponsive chitosan-based hydrogel that gels thanks to the addition of organic and inorganic salt solutions (beta-glycerolphosphate and sodium hydrogen carbonate) and is suitable for cell encapsulation allowing obtaining 3D culture systems. Physico-chemical analyses showed that the hydrogel formulations jellify at physiological conditions (37 °C, pH 7.4), are stable in vitro up to three weeks, have high swelling ratios and mechanical stiffness suitable for cellular encapsulation. Moreover, preliminary biological tests underlined the pronounced biocompatibility of the system. Therefore, these chitosan-based hydrogels are proposed as valid biomaterials for cell encapsulation.https://www.mdpi.com/2076-3417/10/18/6550stimuli-responsive hydrogelnatural polymerscell encapsulationin vitro degradationswelling ratioinjectable hydrogel |
| spellingShingle | Antonella Stanzione Alessandro Polini Velia La Pesa Alessandro Romano Angelo Quattrini Giuseppe Gigli Lorenzo Moroni Francesca Gervaso Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell Encapsulation Applied Sciences stimuli-responsive hydrogel natural polymers cell encapsulation in vitro degradation swelling ratio injectable hydrogel |
| title | Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell Encapsulation |
| title_full | Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell Encapsulation |
| title_fullStr | Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell Encapsulation |
| title_full_unstemmed | Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell Encapsulation |
| title_short | Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell Encapsulation |
| title_sort | development of injectable thermosensitive chitosan based hydrogels for cell encapsulation |
| topic | stimuli-responsive hydrogel natural polymers cell encapsulation in vitro degradation swelling ratio injectable hydrogel |
| url | https://www.mdpi.com/2076-3417/10/18/6550 |
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