Evaluation of Collagen Birefringence in Oral Reactive Lesions Using Picrosirius Red Stain Under Polarized Light Microscopy: An Observational Microscopic Study
Introduction: Oral reactive lesions are relatively common. Collagen is the basic component of such overgrowths that could be fibrous or inflammatory. Hence, understanding the type, nature, and distribution of collagen fibers can aid in our knowledge and better management of these lesions. Materials...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Journal of Orofacial Sciences |
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| Online Access: | http://www.jofs.in/article.asp?issn=0975-8844;year=2021;volume=13;issue=1;spage=47;epage=53;aulast=Shirona |
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| author | A. P. Shirona Usha Hegde H. S. Sreeshyla |
| author_facet | A. P. Shirona Usha Hegde H. S. Sreeshyla |
| author_sort | A. P. Shirona |
| collection | DOAJ |
| description | Introduction: Oral reactive lesions are relatively common. Collagen is the basic component of such overgrowths that could be fibrous or inflammatory. Hence, understanding the type, nature, and distribution of collagen fibers can aid in our knowledge and better management of these lesions. Materials and Methods: Ten paraffin blocks of each of the following lesions − giant-cell granuloma (L1), inflammatory fibrous hyperplasia (L2), pyogenic granuloma (L3), fibroma (L4), and peripheral ossifying fibroma (L5) − were retrieved from the archives. They were analyzed for their collagen birefringence property under polarized microscopy after picrosirius red stain. The demographic details of all the cases were recorded and evaluated. Results: In our study, giant-cell granulomas were commonly observed between third and seventh decades of life, inflammatory fibrous hyperplasias between second and fifth decades of life, yogenic granulomas in sixth decade, fibromas in fifth and sixth decades, and peripheral ossifying fibromas in second and fourth decade of life. All the lesions were predominant in females and commonly observed on gingiva. L5 lesions showed more areas of green birefringence followed by L1, L2, and L3 groups of lesions showed more of red birefringence than other lesions. Mixed birefringence of orange-red and green-yellow was almost same in all the lesions. All these findings were significant statistically. Conclusion: A plausible conclusion that the lesions with mature red fibers have better prognosis than the lesions with immature green fibers, in reactive oral lesions could be drawn. |
| first_indexed | 2024-12-17T23:33:07Z |
| format | Article |
| id | doaj.art-0cdd54db3a894fb6990b814838a68fe4 |
| institution | Directory Open Access Journal |
| issn | 0975-8844 |
| language | English |
| last_indexed | 2024-12-17T23:33:07Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Orofacial Sciences |
| spelling | doaj.art-0cdd54db3a894fb6990b814838a68fe42022-12-21T21:28:37ZengWolters Kluwer Medknow PublicationsJournal of Orofacial Sciences0975-88442021-01-01131475310.4103/jofs.jofs_84_21Evaluation of Collagen Birefringence in Oral Reactive Lesions Using Picrosirius Red Stain Under Polarized Light Microscopy: An Observational Microscopic StudyA. P. ShironaUsha HegdeH. S. SreeshylaIntroduction: Oral reactive lesions are relatively common. Collagen is the basic component of such overgrowths that could be fibrous or inflammatory. Hence, understanding the type, nature, and distribution of collagen fibers can aid in our knowledge and better management of these lesions. Materials and Methods: Ten paraffin blocks of each of the following lesions − giant-cell granuloma (L1), inflammatory fibrous hyperplasia (L2), pyogenic granuloma (L3), fibroma (L4), and peripheral ossifying fibroma (L5) − were retrieved from the archives. They were analyzed for their collagen birefringence property under polarized microscopy after picrosirius red stain. The demographic details of all the cases were recorded and evaluated. Results: In our study, giant-cell granulomas were commonly observed between third and seventh decades of life, inflammatory fibrous hyperplasias between second and fifth decades of life, yogenic granulomas in sixth decade, fibromas in fifth and sixth decades, and peripheral ossifying fibromas in second and fourth decade of life. All the lesions were predominant in females and commonly observed on gingiva. L5 lesions showed more areas of green birefringence followed by L1, L2, and L3 groups of lesions showed more of red birefringence than other lesions. Mixed birefringence of orange-red and green-yellow was almost same in all the lesions. All these findings were significant statistically. Conclusion: A plausible conclusion that the lesions with mature red fibers have better prognosis than the lesions with immature green fibers, in reactive oral lesions could be drawn.http://www.jofs.in/article.asp?issn=0975-8844;year=2021;volume=13;issue=1;spage=47;epage=53;aulast=Shironafibromainflammatory fibrous hyperplasiaoral reactive lesionsperipheral giant-cell granulomaperipheral ossifying fibromapicrosirius red stainpolarized microscopypyogenic granuloma |
| spellingShingle | A. P. Shirona Usha Hegde H. S. Sreeshyla Evaluation of Collagen Birefringence in Oral Reactive Lesions Using Picrosirius Red Stain Under Polarized Light Microscopy: An Observational Microscopic Study Journal of Orofacial Sciences fibroma inflammatory fibrous hyperplasia oral reactive lesions peripheral giant-cell granuloma peripheral ossifying fibroma picrosirius red stain polarized microscopy pyogenic granuloma |
| title | Evaluation of Collagen Birefringence in Oral Reactive Lesions Using Picrosirius Red Stain Under Polarized Light Microscopy: An Observational Microscopic Study |
| title_full | Evaluation of Collagen Birefringence in Oral Reactive Lesions Using Picrosirius Red Stain Under Polarized Light Microscopy: An Observational Microscopic Study |
| title_fullStr | Evaluation of Collagen Birefringence in Oral Reactive Lesions Using Picrosirius Red Stain Under Polarized Light Microscopy: An Observational Microscopic Study |
| title_full_unstemmed | Evaluation of Collagen Birefringence in Oral Reactive Lesions Using Picrosirius Red Stain Under Polarized Light Microscopy: An Observational Microscopic Study |
| title_short | Evaluation of Collagen Birefringence in Oral Reactive Lesions Using Picrosirius Red Stain Under Polarized Light Microscopy: An Observational Microscopic Study |
| title_sort | evaluation of collagen birefringence in oral reactive lesions using picrosirius red stain under polarized light microscopy an observational microscopic study |
| topic | fibroma inflammatory fibrous hyperplasia oral reactive lesions peripheral giant-cell granuloma peripheral ossifying fibroma picrosirius red stain polarized microscopy pyogenic granuloma |
| url | http://www.jofs.in/article.asp?issn=0975-8844;year=2021;volume=13;issue=1;spage=47;epage=53;aulast=Shirona |
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