Self-Assembling Enzymatic Nanocomplexes with Polypeptides and Low-Weight Organic Compounds: Preparation, Characterization, and Application of New Antibacterials
The self-assembling of nanosized materials is a promising field for research and development. Multiple approaches are applied to obtain inorganic, organic and composite nanomaterials with different functionality. In the present work, self-assembling nanocomplexes (NCs) were prepared on the basis of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/1831 |
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| author | Ilya Lyagin Nikolay Stepanov Denis Presnov Artem Trifonov Elena Efremenko |
| author_facet | Ilya Lyagin Nikolay Stepanov Denis Presnov Artem Trifonov Elena Efremenko |
| author_sort | Ilya Lyagin |
| collection | DOAJ |
| description | The self-assembling of nanosized materials is a promising field for research and development. Multiple approaches are applied to obtain inorganic, organic and composite nanomaterials with different functionality. In the present work, self-assembling nanocomplexes (NCs) were prepared on the basis of enzymes and polypeptides followed by the investigation of the influence of low-molecular weight biologically active compounds on the properties of the NCs. For that, the initially possible formation of catalytically active self-assembling NCs of four hydrolytic enzymes with nine effectors was screened via molecular modeling. It allowed the selection of two enzymes (hexahistidine-tagged organophosphorus hydrolase and penicillin acylase) and two compounds (emodin and naringenin) having biological activity. Further, such NCs based on surface-modified enzymes were characterized by a batch of physical and biochemical methods. At least three NCs containing emodin and enzyme (His<sub>6</sub>-OPH and/or penicillin acylase) have been shown to significantly improve the antibacterial activity of colistin and, to a lesser extent, polymyxin B towards both Gram-positive bacteria (<i>Bacillus subtilis</i>) and Gram-negative bacteria (<i>Escherichia coli</i>). |
| first_indexed | 2024-03-11T09:43:27Z |
| format | Article |
| id | doaj.art-0cde0c3baad44d42a620751c019c60d7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:43:27Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0cde0c3baad44d42a620751c019c60d72023-11-16T16:48:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01243183110.3390/ijms24031831Self-Assembling Enzymatic Nanocomplexes with Polypeptides and Low-Weight Organic Compounds: Preparation, Characterization, and Application of New AntibacterialsIlya Lyagin0Nikolay Stepanov1Denis Presnov2Artem Trifonov3Elena Efremenko4Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, RussiaFaculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, RussiaSkobeltsyn Institute of Nuclear Physics, Lomonosov Moscow State University, 119991 Moscow, RussiaFaculty of Physics, Lomonosov Moscow State University, 119991 Moscow, RussiaFaculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, RussiaThe self-assembling of nanosized materials is a promising field for research and development. Multiple approaches are applied to obtain inorganic, organic and composite nanomaterials with different functionality. In the present work, self-assembling nanocomplexes (NCs) were prepared on the basis of enzymes and polypeptides followed by the investigation of the influence of low-molecular weight biologically active compounds on the properties of the NCs. For that, the initially possible formation of catalytically active self-assembling NCs of four hydrolytic enzymes with nine effectors was screened via molecular modeling. It allowed the selection of two enzymes (hexahistidine-tagged organophosphorus hydrolase and penicillin acylase) and two compounds (emodin and naringenin) having biological activity. Further, such NCs based on surface-modified enzymes were characterized by a batch of physical and biochemical methods. At least three NCs containing emodin and enzyme (His<sub>6</sub>-OPH and/or penicillin acylase) have been shown to significantly improve the antibacterial activity of colistin and, to a lesser extent, polymyxin B towards both Gram-positive bacteria (<i>Bacillus subtilis</i>) and Gram-negative bacteria (<i>Escherichia coli</i>).https://www.mdpi.com/1422-0067/24/3/1831nanomaterialenzymemolecular modelingquorum quenchingantibacterialspenicillin acylase |
| spellingShingle | Ilya Lyagin Nikolay Stepanov Denis Presnov Artem Trifonov Elena Efremenko Self-Assembling Enzymatic Nanocomplexes with Polypeptides and Low-Weight Organic Compounds: Preparation, Characterization, and Application of New Antibacterials International Journal of Molecular Sciences nanomaterial enzyme molecular modeling quorum quenching antibacterials penicillin acylase |
| title | Self-Assembling Enzymatic Nanocomplexes with Polypeptides and Low-Weight Organic Compounds: Preparation, Characterization, and Application of New Antibacterials |
| title_full | Self-Assembling Enzymatic Nanocomplexes with Polypeptides and Low-Weight Organic Compounds: Preparation, Characterization, and Application of New Antibacterials |
| title_fullStr | Self-Assembling Enzymatic Nanocomplexes with Polypeptides and Low-Weight Organic Compounds: Preparation, Characterization, and Application of New Antibacterials |
| title_full_unstemmed | Self-Assembling Enzymatic Nanocomplexes with Polypeptides and Low-Weight Organic Compounds: Preparation, Characterization, and Application of New Antibacterials |
| title_short | Self-Assembling Enzymatic Nanocomplexes with Polypeptides and Low-Weight Organic Compounds: Preparation, Characterization, and Application of New Antibacterials |
| title_sort | self assembling enzymatic nanocomplexes with polypeptides and low weight organic compounds preparation characterization and application of new antibacterials |
| topic | nanomaterial enzyme molecular modeling quorum quenching antibacterials penicillin acylase |
| url | https://www.mdpi.com/1422-0067/24/3/1831 |
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