Bacteria in Normal Canine Milk Analyzed by Blood Agar Medium
Studies of microbiota in normal canine milk from healthy dams are sparse. As is the case with blood and urine, it was considered that milk contains no microbiota. Any discovery of bacteria in canine milk is, therefore, often noted to be a result of contamination during sampling or interpreted as mas...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/13/2206 |
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| author | Sabina Sibcic Kolasinac Lars Moe Vibeke Rootwelt Henning Sørum |
| author_facet | Sabina Sibcic Kolasinac Lars Moe Vibeke Rootwelt Henning Sørum |
| author_sort | Sabina Sibcic Kolasinac |
| collection | DOAJ |
| description | Studies of microbiota in normal canine milk from healthy dams are sparse. As is the case with blood and urine, it was considered that milk contains no microbiota. Any discovery of bacteria in canine milk is, therefore, often noted to be a result of contamination during sampling or interpreted as mastitis and treated with antibiotics. Milk was collected twice within 19 days after natural parturition from 11 lactating dams, with no general or local clinical signs of mastitis or other disease. The skin and teats were prepared with an antimicrobial protocol prior to each milk sampling. In total, 210 milk samples were collected and assessed for a number of bacterial colonies grown on each plate. Bacterial growth was detected in 180 samples (86%). <i>Staphylococcus pseudintermedius</i>, <i>Enterococcus</i> spp., <i>Clostridium</i> spp., Coagulase-Negative Staphylococci (CoNS), <i>Streptococcus</i> spp., <i>Streptococcus canis</i>, <i>Bacillus</i> spp., <i>Pasteurella</i> spp., and <i>Escherichia coli</i> were identified from pure and/or mixed bacterial growth, listed in descending order of occurrence. Despite the small sample size, the consistent occurrence of bacteria in early postpartum dams indicates a genuine occurrence of bacteria in canine milk, rather than random contamination. The finding of bacteria in the milk of dams should not, therefore, be the sole argument for the diagnosis of mastitis. |
| first_indexed | 2024-03-11T01:47:43Z |
| format | Article |
| id | doaj.art-0cdf1bbacf814771b7d7695b84f39521 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T01:47:43Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-0cdf1bbacf814771b7d7695b84f395212023-11-18T16:04:33ZengMDPI AGAnimals2076-26152023-07-011313220610.3390/ani13132206Bacteria in Normal Canine Milk Analyzed by Blood Agar MediumSabina Sibcic Kolasinac0Lars Moe1Vibeke Rootwelt2Henning Sørum3Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, 1433 Ås, NorwayDepartment of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, 1433 Ås, NorwayDepartment of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, 1433 Ås, NorwayDepartment of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, 1433 Ås, NorwayStudies of microbiota in normal canine milk from healthy dams are sparse. As is the case with blood and urine, it was considered that milk contains no microbiota. Any discovery of bacteria in canine milk is, therefore, often noted to be a result of contamination during sampling or interpreted as mastitis and treated with antibiotics. Milk was collected twice within 19 days after natural parturition from 11 lactating dams, with no general or local clinical signs of mastitis or other disease. The skin and teats were prepared with an antimicrobial protocol prior to each milk sampling. In total, 210 milk samples were collected and assessed for a number of bacterial colonies grown on each plate. Bacterial growth was detected in 180 samples (86%). <i>Staphylococcus pseudintermedius</i>, <i>Enterococcus</i> spp., <i>Clostridium</i> spp., Coagulase-Negative Staphylococci (CoNS), <i>Streptococcus</i> spp., <i>Streptococcus canis</i>, <i>Bacillus</i> spp., <i>Pasteurella</i> spp., and <i>Escherichia coli</i> were identified from pure and/or mixed bacterial growth, listed in descending order of occurrence. Despite the small sample size, the consistent occurrence of bacteria in early postpartum dams indicates a genuine occurrence of bacteria in canine milk, rather than random contamination. The finding of bacteria in the milk of dams should not, therefore, be the sole argument for the diagnosis of mastitis.https://www.mdpi.com/2076-2615/13/13/2206culturable microbiomedoglactationmammary glandcanine milk bacteriome |
| spellingShingle | Sabina Sibcic Kolasinac Lars Moe Vibeke Rootwelt Henning Sørum Bacteria in Normal Canine Milk Analyzed by Blood Agar Medium Animals culturable microbiome dog lactation mammary gland canine milk bacteriome |
| title | Bacteria in Normal Canine Milk Analyzed by Blood Agar Medium |
| title_full | Bacteria in Normal Canine Milk Analyzed by Blood Agar Medium |
| title_fullStr | Bacteria in Normal Canine Milk Analyzed by Blood Agar Medium |
| title_full_unstemmed | Bacteria in Normal Canine Milk Analyzed by Blood Agar Medium |
| title_short | Bacteria in Normal Canine Milk Analyzed by Blood Agar Medium |
| title_sort | bacteria in normal canine milk analyzed by blood agar medium |
| topic | culturable microbiome dog lactation mammary gland canine milk bacteriome |
| url | https://www.mdpi.com/2076-2615/13/13/2206 |
| work_keys_str_mv | AT sabinasibcickolasinac bacteriainnormalcaninemilkanalyzedbybloodagarmedium AT larsmoe bacteriainnormalcaninemilkanalyzedbybloodagarmedium AT vibekerootwelt bacteriainnormalcaninemilkanalyzedbybloodagarmedium AT henningsørum bacteriainnormalcaninemilkanalyzedbybloodagarmedium |