Mutant Parkin impairs mitochondrial function and morphology in human fibroblasts.
Mutations in Parkin are the most common cause of autosomal recessive Parkinson disease (PD). The mitochondrially localized E3 ubiquitin-protein ligase Parkin has been reported to be involved in respiratory chain function and mitochondrial dynamics. More recent publications also described a link betw...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2010-09-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2946349?pdf=render |
| _version_ | 1818557708327976960 |
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| author | Anne Grünewald Lisa Voges Aleksandar Rakovic Meike Kasten Himesha Vandebona Claudia Hemmelmann Katja Lohmann Slobodanka Orolicki Alfredo Ramirez Anthony H V Schapira Peter P Pramstaller Carolyn M Sue Christine Klein |
| author_facet | Anne Grünewald Lisa Voges Aleksandar Rakovic Meike Kasten Himesha Vandebona Claudia Hemmelmann Katja Lohmann Slobodanka Orolicki Alfredo Ramirez Anthony H V Schapira Peter P Pramstaller Carolyn M Sue Christine Klein |
| author_sort | Anne Grünewald |
| collection | DOAJ |
| description | Mutations in Parkin are the most common cause of autosomal recessive Parkinson disease (PD). The mitochondrially localized E3 ubiquitin-protein ligase Parkin has been reported to be involved in respiratory chain function and mitochondrial dynamics. More recent publications also described a link between Parkin and mitophagy.In this study, we investigated the impact of Parkin mutations on mitochondrial function and morphology in a human cellular model. Fibroblasts were obtained from three members of an Italian PD family with two mutations in Parkin (homozygous c.1072delT, homozygous delEx7, compound-heterozygous c.1072delT/delEx7), as well as from two relatives without mutations. Furthermore, three unrelated compound-heterozygous patients (delEx3-4/duplEx7-12, delEx4/c.924C>T and delEx1/c.924C>T) and three unrelated age-matched controls were included. Fibroblasts were cultured under basal or paraquat-induced oxidative stress conditions. ATP synthesis rates and cellular levels were detected luminometrically. Activities of complexes I-IV and citrate synthase were measured spectrophotometrically in mitochondrial preparations or cell lysates. The mitochondrial membrane potential was measured with 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide. Oxidative stress levels were investigated with the OxyBlot technique. The mitochondrial network was investigated immunocytochemically and the degree of branching was determined with image processing methods. We observed a decrease in the production and overall concentration of ATP coinciding with increased mitochondrial mass in Parkin-mutant fibroblasts. After an oxidative insult, the membrane potential decreased in patient cells but not in controls. We further determined higher levels of oxidized proteins in the mutants both under basal and stress conditions. The degree of mitochondrial network branching was comparable in mutants and controls under basal conditions and decreased to a similar extent under paraquat-induced stress.Our results indicate that Parkin mutations cause abnormal mitochondrial function and morphology in non-neuronal human cells. |
| first_indexed | 2024-12-14T00:03:06Z |
| format | Article |
| id | doaj.art-0cdf43fbc1dd4d02a28dc1e66683c592 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T00:03:06Z |
| publishDate | 2010-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0cdf43fbc1dd4d02a28dc1e66683c5922022-12-21T23:26:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-09-0159e1296210.1371/journal.pone.0012962Mutant Parkin impairs mitochondrial function and morphology in human fibroblasts.Anne GrünewaldLisa VogesAleksandar RakovicMeike KastenHimesha VandebonaClaudia HemmelmannKatja LohmannSlobodanka OrolickiAlfredo RamirezAnthony H V SchapiraPeter P PramstallerCarolyn M SueChristine KleinMutations in Parkin are the most common cause of autosomal recessive Parkinson disease (PD). The mitochondrially localized E3 ubiquitin-protein ligase Parkin has been reported to be involved in respiratory chain function and mitochondrial dynamics. More recent publications also described a link between Parkin and mitophagy.In this study, we investigated the impact of Parkin mutations on mitochondrial function and morphology in a human cellular model. Fibroblasts were obtained from three members of an Italian PD family with two mutations in Parkin (homozygous c.1072delT, homozygous delEx7, compound-heterozygous c.1072delT/delEx7), as well as from two relatives without mutations. Furthermore, three unrelated compound-heterozygous patients (delEx3-4/duplEx7-12, delEx4/c.924C>T and delEx1/c.924C>T) and three unrelated age-matched controls were included. Fibroblasts were cultured under basal or paraquat-induced oxidative stress conditions. ATP synthesis rates and cellular levels were detected luminometrically. Activities of complexes I-IV and citrate synthase were measured spectrophotometrically in mitochondrial preparations or cell lysates. The mitochondrial membrane potential was measured with 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide. Oxidative stress levels were investigated with the OxyBlot technique. The mitochondrial network was investigated immunocytochemically and the degree of branching was determined with image processing methods. We observed a decrease in the production and overall concentration of ATP coinciding with increased mitochondrial mass in Parkin-mutant fibroblasts. After an oxidative insult, the membrane potential decreased in patient cells but not in controls. We further determined higher levels of oxidized proteins in the mutants both under basal and stress conditions. The degree of mitochondrial network branching was comparable in mutants and controls under basal conditions and decreased to a similar extent under paraquat-induced stress.Our results indicate that Parkin mutations cause abnormal mitochondrial function and morphology in non-neuronal human cells.http://europepmc.org/articles/PMC2946349?pdf=render |
| spellingShingle | Anne Grünewald Lisa Voges Aleksandar Rakovic Meike Kasten Himesha Vandebona Claudia Hemmelmann Katja Lohmann Slobodanka Orolicki Alfredo Ramirez Anthony H V Schapira Peter P Pramstaller Carolyn M Sue Christine Klein Mutant Parkin impairs mitochondrial function and morphology in human fibroblasts. PLoS ONE |
| title | Mutant Parkin impairs mitochondrial function and morphology in human fibroblasts. |
| title_full | Mutant Parkin impairs mitochondrial function and morphology in human fibroblasts. |
| title_fullStr | Mutant Parkin impairs mitochondrial function and morphology in human fibroblasts. |
| title_full_unstemmed | Mutant Parkin impairs mitochondrial function and morphology in human fibroblasts. |
| title_short | Mutant Parkin impairs mitochondrial function and morphology in human fibroblasts. |
| title_sort | mutant parkin impairs mitochondrial function and morphology in human fibroblasts |
| url | http://europepmc.org/articles/PMC2946349?pdf=render |
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