Measurement of Rapid Protein Diffusion in the Cytoplasm by Photo-Converted Intensity Profile Expansion

Measurement of Rapid Protein Diffusion in the Cytoplasm by Photo-Converted Intensity Profile Expansion

The fluorescence microscopy methods presently used to characterize protein motion in cells infer protein motion from indirect observables, rather than measuring protein motion directly. Operationalizing these methods requires expertise that can constitute a barrier to their broad utilization. Here,...

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Bibliographic Details
Main Authors: Rotem Gura Sadovsky, Shlomi Brielle, Daniel Kaganovich, Jeremy L. England
Format: Article
Language:English
Published: Elsevier 2017-03-01
Series:Cell Reports
Subjects:
fluorescence-based techniques
molecular diffusion
protein mobility
photo-conversion
anomalous diffusion
molecular crowding
protein oligomerization
protein quality control
protein aggregation
Sod1
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124717302784

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