Epigenetic silencing of miR-203 in Kazakh patients with esophageal squamous cell carcinoma by MassARRAY spectrometry
Dysregulation of miR-203 by promoter methylation is associated with the development of various cancers. We aimed to explore the underlying link between promoter methylation and miR-203 expression in Kazakh esophageal squamous cell carcinoma (ESCC). MassARRAY® System spectrometry was used to quantita...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-08-01
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| Series: | Epigenetics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592294.2017.1349045 |
| _version_ | 1797678934697967616 |
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| author | Xiaobin Cui Xi Chen Weiwei Wang Aimin Chang Lan Yang Chunxia Liu Hao Peng Yutao Wei Weihua Liang Shugang Li Ning Wang Wei Liu Jianming Hu Wenjie Zhang Lidong Wang Yunzhao Chen Feng Li |
| author_facet | Xiaobin Cui Xi Chen Weiwei Wang Aimin Chang Lan Yang Chunxia Liu Hao Peng Yutao Wei Weihua Liang Shugang Li Ning Wang Wei Liu Jianming Hu Wenjie Zhang Lidong Wang Yunzhao Chen Feng Li |
| author_sort | Xiaobin Cui |
| collection | DOAJ |
| description | Dysregulation of miR-203 by promoter methylation is associated with the development of various cancers. We aimed to explore the underlying link between promoter methylation and miR-203 expression in Kazakh esophageal squamous cell carcinoma (ESCC). MassARRAY® System spectrometry was used to quantitatively analyze the DNA methylation of 32 CpG sites within miR-203 in 99 Kazakh ESCC and 46 normal esophageal tissues (NETs) with similar population characteristics. We conducted real-time PCR to detect miR-203 expression levels and evaluated their association with methylation. Eleven CpG units within miR-203 promoter were frequently hypermethylated in ESCC compared with NETs (P < 0.05). The hypermethylation of several CpG units positively correlated with age, lower esophagus, constrictive type of ESCC, and moderately differentiated ESCC. Given the involvement of human papillomavirus (HPV) in etiology of ESCC was confirmed from our previous reports, herein we found that CpG units within miR-203 in HPV16-positive ESCC are more heavily methylated. Furthermore, miR-203 expression showed a nearly 4.5-fold decrease in ESCC than NETs (0.206 ± 0.336 vs. 0.908 ± 1.424, P < 0.001) and was significantly associated with lymph node metastasis (P = 0.012). The expression of miR-203 with 11 completely hypermethylated CpG units was approximately 6.5-fold lower than that with at least 1 unmethylated CpG unit (P < 0.001) and especially the CpG_15.16 and CpG_31.32 with higher methylation levels in ESCC tissues exhibited lower expression levels of miR-203, which indicated a reverse association between miR-203 methylation and expression. Hypermethylated miR-203 is a potential biomarker and targeted delivery of miR-203 could therefore serve as a preventive or therapeutic strategy for Kazakh ESCC. |
| first_indexed | 2024-03-11T23:07:06Z |
| format | Article |
| id | doaj.art-0ce145ee738b492395402a5a3346d9da |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:07:06Z |
| publishDate | 2017-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-0ce145ee738b492395402a5a3346d9da2023-09-21T12:43:13ZengTaylor & Francis GroupEpigenetics1559-22941559-23082017-08-0112869870710.1080/15592294.2017.13490451349045Epigenetic silencing of miR-203 in Kazakh patients with esophageal squamous cell carcinoma by MassARRAY spectrometryXiaobin Cui0Xi Chen1Weiwei Wang2Aimin Chang3Lan Yang4Chunxia Liu5Hao Peng6Yutao Wei7Weihua Liang8Shugang Li9Ning Wang10Wei Liu11Jianming Hu12Wenjie Zhang13Lidong Wang14Yunzhao Chen15Feng Li16Shihezi University School of MedicineShihezi University School of MedicineZhucheng Maternal and Child Care Service CentrePeople's Hospital of WusuShihezi University School of MedicineShihezi University School of MedicineShihezi University School of MedicineThe First Affiliated Hospital, Shihezi University School of MedicineShihezi University School of MedicineShihezi University School of MedicineShihezi University School of MedicineShihezi University School of MedicineShihezi University School of MedicineShihezi University School of MedicineHenan Key Laboratory for Esophageal Cancer ResearchShihezi University School of MedicineShihezi University School of MedicineDysregulation of miR-203 by promoter methylation is associated with the development of various cancers. We aimed to explore the underlying link between promoter methylation and miR-203 expression in Kazakh esophageal squamous cell carcinoma (ESCC). MassARRAY® System spectrometry was used to quantitatively analyze the DNA methylation of 32 CpG sites within miR-203 in 99 Kazakh ESCC and 46 normal esophageal tissues (NETs) with similar population characteristics. We conducted real-time PCR to detect miR-203 expression levels and evaluated their association with methylation. Eleven CpG units within miR-203 promoter were frequently hypermethylated in ESCC compared with NETs (P < 0.05). The hypermethylation of several CpG units positively correlated with age, lower esophagus, constrictive type of ESCC, and moderately differentiated ESCC. Given the involvement of human papillomavirus (HPV) in etiology of ESCC was confirmed from our previous reports, herein we found that CpG units within miR-203 in HPV16-positive ESCC are more heavily methylated. Furthermore, miR-203 expression showed a nearly 4.5-fold decrease in ESCC than NETs (0.206 ± 0.336 vs. 0.908 ± 1.424, P < 0.001) and was significantly associated with lymph node metastasis (P = 0.012). The expression of miR-203 with 11 completely hypermethylated CpG units was approximately 6.5-fold lower than that with at least 1 unmethylated CpG unit (P < 0.001) and especially the CpG_15.16 and CpG_31.32 with higher methylation levels in ESCC tissues exhibited lower expression levels of miR-203, which indicated a reverse association between miR-203 methylation and expression. Hypermethylated miR-203 is a potential biomarker and targeted delivery of miR-203 could therefore serve as a preventive or therapeutic strategy for Kazakh ESCC.http://dx.doi.org/10.1080/15592294.2017.1349045esophageal squamous cell carcinomakazakhmir-203methylation |
| spellingShingle | Xiaobin Cui Xi Chen Weiwei Wang Aimin Chang Lan Yang Chunxia Liu Hao Peng Yutao Wei Weihua Liang Shugang Li Ning Wang Wei Liu Jianming Hu Wenjie Zhang Lidong Wang Yunzhao Chen Feng Li Epigenetic silencing of miR-203 in Kazakh patients with esophageal squamous cell carcinoma by MassARRAY spectrometry Epigenetics esophageal squamous cell carcinoma kazakh mir-203 methylation |
| title | Epigenetic silencing of miR-203 in Kazakh patients with esophageal squamous cell carcinoma by MassARRAY spectrometry |
| title_full | Epigenetic silencing of miR-203 in Kazakh patients with esophageal squamous cell carcinoma by MassARRAY spectrometry |
| title_fullStr | Epigenetic silencing of miR-203 in Kazakh patients with esophageal squamous cell carcinoma by MassARRAY spectrometry |
| title_full_unstemmed | Epigenetic silencing of miR-203 in Kazakh patients with esophageal squamous cell carcinoma by MassARRAY spectrometry |
| title_short | Epigenetic silencing of miR-203 in Kazakh patients with esophageal squamous cell carcinoma by MassARRAY spectrometry |
| title_sort | epigenetic silencing of mir 203 in kazakh patients with esophageal squamous cell carcinoma by massarray spectrometry |
| topic | esophageal squamous cell carcinoma kazakh mir-203 methylation |
| url | http://dx.doi.org/10.1080/15592294.2017.1349045 |
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