Isolation and pathogenicity of <em>Phytophthora</em> species and <em>Phytopythium vexans</em> recovered from avocado orchards in the Canary Islands, including <em>Phytophthora niederhauserii</em> as a new pathogen of avocado
Root rot, caused by Phytophthora cinnamomi, is the most important disease of avocado, but few studies have determined whether other Phytophthora or oomycete species are involved in crop decline. Avocado orchards in the Canary Islands were surveyed for the presence of Phytophthora and Phytophthora-li...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Firenze University Press
2018-05-01
|
| Series: | Phytopathologia Mediterranea |
| Subjects: | |
| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/5752 |
| _version_ | 1811276497416093696 |
|---|---|
| author | Cristina RODRÍGUEZ-PADRÓN Felipe SIVERIO Ana PÉREZ-SIERRA Ana RODRÍGUEZ |
| author_facet | Cristina RODRÍGUEZ-PADRÓN Felipe SIVERIO Ana PÉREZ-SIERRA Ana RODRÍGUEZ |
| author_sort | Cristina RODRÍGUEZ-PADRÓN |
| collection | DOAJ |
| description | Root rot, caused by Phytophthora cinnamomi, is the most important disease of avocado, but few studies have determined whether other Phytophthora or oomycete species are involved in crop decline. Avocado orchards in the Canary Islands were surveyed for the presence of Phytophthora and Phytophthora-like oomycetes. Isolates obtained were identified morphologically and by sequence analysis of their internal transcribed spacer (ITS) regions, and their pathogenicity was tested by root and stem inoculation of avocado seedlings. Phytophthora species were isolated in 41 of 99 orchards sampled, and 10% of orchards were infected with more than one species. The species most frequently isolated was P. cinnamomi, which was detected in 26 orchards. In addition, P. multivora (ten orchards), P. niederhauserii (four orchards), P. nicotianae (four orchards), P. palmivora (one orchard) and Phytopythium vexans (20 orchards) were isolated. Phytophthora nicotianae and P. palmivora have been previously reported as pathogens of avocado, but P. niederhauserii, P. multivora and Pp. vexans are reported for the first time to be associated with this host. Phytophthora niederhauserii was the most virulent of these species. It was isolated from declining trees, and root rot severity was comparable to that caused by P. cinnamomi in two independent pathogenicity tests. In addition, P. niederhauserii caused cankers after stem inoculation. The pathogenicity results for P. multivora and Pp. vexans varied depending on isolates and pathogenicity tests. This study increases the knowledge of oomycetes associated with avocado, highlighting the potential threat posed by P. niederhauserii to this important fruit crop. |
| first_indexed | 2024-04-12T23:58:43Z |
| format | Article |
| id | doaj.art-0ce1e3db45ef44dbbba073426da7220a |
| institution | Directory Open Access Journal |
| issn | 0031-9465 1593-2095 |
| language | English |
| last_indexed | 2024-04-12T23:58:43Z |
| publishDate | 2018-05-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj.art-0ce1e3db45ef44dbbba073426da7220a2022-12-22T03:11:25ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952018-05-0157110.14601/Phytopathol_Mediterr-2202218966Isolation and pathogenicity of <em>Phytophthora</em> species and <em>Phytopythium vexans</em> recovered from avocado orchards in the Canary Islands, including <em>Phytophthora niederhauserii</em> as a new pathogen of avocadoCristina RODRÍGUEZ-PADRÓN0Felipe SIVERIO1Ana PÉREZ-SIERRA2Ana RODRÍGUEZ3Departamento de Protección Vegetal, Instituto Canario de Investigaciones Agrarias (ICIA). Apdo. 60, 38200 La Laguna, Tenerife, Canary Islands, SpainDepartamento de Protección Vegetal, Instituto Canario de Investigaciones Agrarias (ICIA). Apdo. 60, 38200 La Laguna, Tenerife, Canary Islands, Spain Sección de Laboratorio de Sanidad Vegetal de la Consejería de Agricultura, Ganadería, Pesca y Aguas del Gobierno de Canarias, Ctra. El Boquerón, s/n, Tenerife, Canary Islands, SpainForest Research, Alice Holt Lodge, Farnham, Surrey, GU10 4LH, UKDepartamento de Bioquímica, Microbiología, Biología Celular y Genética. Facultad de Farmacia. Universidad de La Laguna 38206 La Laguna, Tenerife, Canary Islands, SpainRoot rot, caused by Phytophthora cinnamomi, is the most important disease of avocado, but few studies have determined whether other Phytophthora or oomycete species are involved in crop decline. Avocado orchards in the Canary Islands were surveyed for the presence of Phytophthora and Phytophthora-like oomycetes. Isolates obtained were identified morphologically and by sequence analysis of their internal transcribed spacer (ITS) regions, and their pathogenicity was tested by root and stem inoculation of avocado seedlings. Phytophthora species were isolated in 41 of 99 orchards sampled, and 10% of orchards were infected with more than one species. The species most frequently isolated was P. cinnamomi, which was detected in 26 orchards. In addition, P. multivora (ten orchards), P. niederhauserii (four orchards), P. nicotianae (four orchards), P. palmivora (one orchard) and Phytopythium vexans (20 orchards) were isolated. Phytophthora nicotianae and P. palmivora have been previously reported as pathogens of avocado, but P. niederhauserii, P. multivora and Pp. vexans are reported for the first time to be associated with this host. Phytophthora niederhauserii was the most virulent of these species. It was isolated from declining trees, and root rot severity was comparable to that caused by P. cinnamomi in two independent pathogenicity tests. In addition, P. niederhauserii caused cankers after stem inoculation. The pathogenicity results for P. multivora and Pp. vexans varied depending on isolates and pathogenicity tests. This study increases the knowledge of oomycetes associated with avocado, highlighting the potential threat posed by P. niederhauserii to this important fruit crop.https://oajournals.fupress.net/index.php/pm/article/view/5752Persea americanaPhytophthora cinnamomiPhytophthora multivoraPhytopythium |
| spellingShingle | Cristina RODRÍGUEZ-PADRÓN Felipe SIVERIO Ana PÉREZ-SIERRA Ana RODRÍGUEZ Isolation and pathogenicity of <em>Phytophthora</em> species and <em>Phytopythium vexans</em> recovered from avocado orchards in the Canary Islands, including <em>Phytophthora niederhauserii</em> as a new pathogen of avocado Phytopathologia Mediterranea Persea americana Phytophthora cinnamomi Phytophthora multivora Phytopythium |
| title | Isolation and pathogenicity of <em>Phytophthora</em> species and <em>Phytopythium vexans</em> recovered from avocado orchards in the Canary Islands, including <em>Phytophthora niederhauserii</em> as a new pathogen of avocado |
| title_full | Isolation and pathogenicity of <em>Phytophthora</em> species and <em>Phytopythium vexans</em> recovered from avocado orchards in the Canary Islands, including <em>Phytophthora niederhauserii</em> as a new pathogen of avocado |
| title_fullStr | Isolation and pathogenicity of <em>Phytophthora</em> species and <em>Phytopythium vexans</em> recovered from avocado orchards in the Canary Islands, including <em>Phytophthora niederhauserii</em> as a new pathogen of avocado |
| title_full_unstemmed | Isolation and pathogenicity of <em>Phytophthora</em> species and <em>Phytopythium vexans</em> recovered from avocado orchards in the Canary Islands, including <em>Phytophthora niederhauserii</em> as a new pathogen of avocado |
| title_short | Isolation and pathogenicity of <em>Phytophthora</em> species and <em>Phytopythium vexans</em> recovered from avocado orchards in the Canary Islands, including <em>Phytophthora niederhauserii</em> as a new pathogen of avocado |
| title_sort | isolation and pathogenicity of em phytophthora em species and em phytopythium vexans em recovered from avocado orchards in the canary islands including em phytophthora niederhauserii em as a new pathogen of avocado |
| topic | Persea americana Phytophthora cinnamomi Phytophthora multivora Phytopythium |
| url | https://oajournals.fupress.net/index.php/pm/article/view/5752 |
| work_keys_str_mv | AT cristinarodriguezpadron isolationandpathogenicityofemphytophthoraemspeciesandemphytopythiumvexansemrecoveredfromavocadoorchardsinthecanaryislandsincludingemphytophthoraniederhauseriiemasanewpathogenofavocado AT felipesiverio isolationandpathogenicityofemphytophthoraemspeciesandemphytopythiumvexansemrecoveredfromavocadoorchardsinthecanaryislandsincludingemphytophthoraniederhauseriiemasanewpathogenofavocado AT anaperezsierra isolationandpathogenicityofemphytophthoraemspeciesandemphytopythiumvexansemrecoveredfromavocadoorchardsinthecanaryislandsincludingemphytophthoraniederhauseriiemasanewpathogenofavocado AT anarodriguez isolationandpathogenicityofemphytophthoraemspeciesandemphytopythiumvexansemrecoveredfromavocadoorchardsinthecanaryislandsincludingemphytophthoraniederhauseriiemasanewpathogenofavocado |