The Tannat genome: Unravelling its unique characteristics
Tannat (Vitis vinifera) is the most cultivated grapevine variety in Uruguay for the production of high quality red wines. Its berries have unusually high levels of polyphenolic compounds (anthocyanins and tannins), producing wines with intense purple colour and high antioxidant properties. Remarkabl...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2019-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/full_html/2019/01/bioconf-oiv2018_01016/bioconf-oiv2018_01016.html |
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| author | Da Silva C. Molin A.D. Ferrarini A. Boido E. Gaggero C. Delledonne M. Carrau F. |
| author_facet | Da Silva C. Molin A.D. Ferrarini A. Boido E. Gaggero C. Delledonne M. Carrau F. |
| author_sort | Da Silva C. |
| collection | DOAJ |
| description | Tannat (Vitis vinifera) is the most cultivated grapevine variety in Uruguay for the production of high quality red wines. Its berries have unusually high levels of polyphenolic compounds (anthocyanins and tannins), producing wines with intense purple colour and high antioxidant properties. Remarkably, more than 40% of its tannins are galloylated, which determines a greater antioxidant power. Technologies of massive sequencing allow the characterization of genomic variants between different clutivars. The Tannat genome was sequenced with a 134X coverage using the Illumina technology, and was annotated using transcriptomes (RNA-Seq) of different berry tissues. When comparing the genomes of Tannat with Pinot Noir PN40024 (reference genome) we found an expansion of the gene families related to the biosynthesis of polyphenols. A search base on the recently reported epicatechin galloyl transferase (ECGT) from tea leaves determined five putative genes encoding the ECGT in Tannat. Genetic analysis of one of the transcription factor that regulates the anthocyanin synthesis during berry ripening, VvMYBA1, shows the presence of Gret1 retrotransposon in one of the VvMYBA1 alleles in the Tannat clones analysed. This work makes original contributions about the molecular bases of the biosynthesis of anthocyanins and tannins during the development of the flagship grape of Uruguay. |
| first_indexed | 2024-12-13T13:49:45Z |
| format | Article |
| id | doaj.art-0ce9cb988c59479fb93299f72d7035d2 |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-12-13T13:49:45Z |
| publishDate | 2019-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-0ce9cb988c59479fb93299f72d7035d22022-12-21T23:43:16ZengEDP SciencesBIO Web of Conferences2117-44582019-01-01120101610.1051/bioconf/20191201016bioconf-oiv2018_01016The Tannat genome: Unravelling its unique characteristicsDa Silva C.0Molin A.D.1Ferrarini A.2Boido E.3Gaggero C.4Delledonne M.5Carrau F.6PDU Espacio de Biología Vegetal del Noreste, Centro Universitario de Tacuarembó, Universidad de la RepúblicaCentro di Genomica Funzionale, Dipartimento di Biotecnologie, Universitá degli Studi di VeronaCentro di Genomica Funzionale, Dipartimento di Biotecnologie, Universitá degli Studi di VeronaÁrea Enología y Biotecnología de Fermentación, Facultad de Química, Universidad de la RepúblicaDepartamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente EstableCentro di Genomica Funzionale, Dipartimento di Biotecnologie, Universitá degli Studi di VeronaÁrea Enología y Biotecnología de Fermentación, Facultad de Química, Universidad de la RepúblicaTannat (Vitis vinifera) is the most cultivated grapevine variety in Uruguay for the production of high quality red wines. Its berries have unusually high levels of polyphenolic compounds (anthocyanins and tannins), producing wines with intense purple colour and high antioxidant properties. Remarkably, more than 40% of its tannins are galloylated, which determines a greater antioxidant power. Technologies of massive sequencing allow the characterization of genomic variants between different clutivars. The Tannat genome was sequenced with a 134X coverage using the Illumina technology, and was annotated using transcriptomes (RNA-Seq) of different berry tissues. When comparing the genomes of Tannat with Pinot Noir PN40024 (reference genome) we found an expansion of the gene families related to the biosynthesis of polyphenols. A search base on the recently reported epicatechin galloyl transferase (ECGT) from tea leaves determined five putative genes encoding the ECGT in Tannat. Genetic analysis of one of the transcription factor that regulates the anthocyanin synthesis during berry ripening, VvMYBA1, shows the presence of Gret1 retrotransposon in one of the VvMYBA1 alleles in the Tannat clones analysed. This work makes original contributions about the molecular bases of the biosynthesis of anthocyanins and tannins during the development of the flagship grape of Uruguay.https://www.bio-conferences.org/articles/bioconf/full_html/2019/01/bioconf-oiv2018_01016/bioconf-oiv2018_01016.html |
| spellingShingle | Da Silva C. Molin A.D. Ferrarini A. Boido E. Gaggero C. Delledonne M. Carrau F. The Tannat genome: Unravelling its unique characteristics BIO Web of Conferences |
| title | The Tannat genome: Unravelling its unique characteristics |
| title_full | The Tannat genome: Unravelling its unique characteristics |
| title_fullStr | The Tannat genome: Unravelling its unique characteristics |
| title_full_unstemmed | The Tannat genome: Unravelling its unique characteristics |
| title_short | The Tannat genome: Unravelling its unique characteristics |
| title_sort | tannat genome unravelling its unique characteristics |
| url | https://www.bio-conferences.org/articles/bioconf/full_html/2019/01/bioconf-oiv2018_01016/bioconf-oiv2018_01016.html |
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