Which stem cells to choose for regenerative medicine application: Bone marrow and adipose tissue stromal stem cells – Similarities and differences
Background: Clinical use of stromal stem cells in regenerative medicine is increasingly recognized as a promising treatment modality for age-related degenerative diseases based on the promising initial results of clinical trials. However, the magnitude of positive effects observed in these trials ha...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Journal of Nature and Science of Medicine |
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| Online Access: | http://www.jnsmonline.org/article.asp?issn=2589-627X;year=2018;volume=1;issue=2;spage=48;epage=54;aulast=Alajez |
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| author | Nehad M Alajez Dalia Al-Ali Radhakrishnan Vishnubalaji Muthurangan Manikandan Musaad Alfayez Moustapha Kassem Abdullah Aldahmash |
| author_facet | Nehad M Alajez Dalia Al-Ali Radhakrishnan Vishnubalaji Muthurangan Manikandan Musaad Alfayez Moustapha Kassem Abdullah Aldahmash |
| author_sort | Nehad M Alajez |
| collection | DOAJ |
| description | Background: Clinical use of stromal stem cells in regenerative medicine is increasingly recognized as a promising treatment modality for age-related degenerative diseases based on the promising initial results of clinical trials. However, the magnitude of positive effects observed in these trials has been variable which can be explained by the lack of standardization of the stem cell products “cell product.” Bone marrow-derived stromal (also known mesenchymal) stem cells (BM-hMSC) and adipose tissue-hMSC (AD-hMSC) have been used interchangeably in clinical trials employing stromal stem cells as they were thought to be functionally identical. Methods: In the present study, we performed an extensive side-by-side comparison of BM-hMSC and AD-hMSC for their CD marker expression using FACS analysis, molecular phenotype using global mRNA gene expression analysis, and functional studies for their in vitro differentiation capacity to osteoblasts and adipocytes. Results: We observed both stromal cell populations were CD44+ CD13+ CD90+ CD29+ CD105+ CD14− HLDR−. We also observed that they express common genetic signature consisting of 13,667 genes with enrichment in a number of pathways relevant to stem cell biology, for example, focal adhesion, insulin signaling, and mitogen-activated protein kinase signaling. On the other hand, we observed significant differences in their molecular phenotype with 3282 and 1409 genes differentially expression in BM-hMSC and AD-hMSC, respectively. Further analysis revealed higher expression of genes associated with osteoblast differentiation in BM-hMSC and those of adipocyte differentiation in AD-hMSC which correlated with their differential capacity for osteoblast versus adipocyte differentiation, respectively. Conclusion: Our data suggest that the clinical use of MSC in therapy depend on MSC site of origin, and thus, BM-hMSC are better suited for clinical trials aiming at enhancing bone regeneration. We suggest that molecular phenotype of stem cells is relevant approach for stem cell screening before their clinical transplantation. |
| first_indexed | 2024-04-14T00:43:56Z |
| format | Article |
| id | doaj.art-0cf4115955bc48bdb6dd9ff386fe8a14 |
| institution | Directory Open Access Journal |
| issn | 2589-627X 2589-6288 |
| language | English |
| last_indexed | 2024-04-14T00:43:56Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Nature and Science of Medicine |
| spelling | doaj.art-0cf4115955bc48bdb6dd9ff386fe8a142022-12-22T02:22:04ZengWolters Kluwer Medknow PublicationsJournal of Nature and Science of Medicine2589-627X2589-62882018-01-0112485410.4103/JNSM.JNSM_18_18Which stem cells to choose for regenerative medicine application: Bone marrow and adipose tissue stromal stem cells – Similarities and differencesNehad M AlajezDalia Al-AliRadhakrishnan VishnubalajiMuthurangan ManikandanMusaad AlfayezMoustapha KassemAbdullah AldahmashBackground: Clinical use of stromal stem cells in regenerative medicine is increasingly recognized as a promising treatment modality for age-related degenerative diseases based on the promising initial results of clinical trials. However, the magnitude of positive effects observed in these trials has been variable which can be explained by the lack of standardization of the stem cell products “cell product.” Bone marrow-derived stromal (also known mesenchymal) stem cells (BM-hMSC) and adipose tissue-hMSC (AD-hMSC) have been used interchangeably in clinical trials employing stromal stem cells as they were thought to be functionally identical. Methods: In the present study, we performed an extensive side-by-side comparison of BM-hMSC and AD-hMSC for their CD marker expression using FACS analysis, molecular phenotype using global mRNA gene expression analysis, and functional studies for their in vitro differentiation capacity to osteoblasts and adipocytes. Results: We observed both stromal cell populations were CD44+ CD13+ CD90+ CD29+ CD105+ CD14− HLDR−. We also observed that they express common genetic signature consisting of 13,667 genes with enrichment in a number of pathways relevant to stem cell biology, for example, focal adhesion, insulin signaling, and mitogen-activated protein kinase signaling. On the other hand, we observed significant differences in their molecular phenotype with 3282 and 1409 genes differentially expression in BM-hMSC and AD-hMSC, respectively. Further analysis revealed higher expression of genes associated with osteoblast differentiation in BM-hMSC and those of adipocyte differentiation in AD-hMSC which correlated with their differential capacity for osteoblast versus adipocyte differentiation, respectively. Conclusion: Our data suggest that the clinical use of MSC in therapy depend on MSC site of origin, and thus, BM-hMSC are better suited for clinical trials aiming at enhancing bone regeneration. We suggest that molecular phenotype of stem cells is relevant approach for stem cell screening before their clinical transplantation.http://www.jnsmonline.org/article.asp?issn=2589-627X;year=2018;volume=1;issue=2;spage=48;epage=54;aulast=Alajezadipose tissuebone marrowmesenchymal stromal cellsosteogenesispathways |
| spellingShingle | Nehad M Alajez Dalia Al-Ali Radhakrishnan Vishnubalaji Muthurangan Manikandan Musaad Alfayez Moustapha Kassem Abdullah Aldahmash Which stem cells to choose for regenerative medicine application: Bone marrow and adipose tissue stromal stem cells – Similarities and differences Journal of Nature and Science of Medicine adipose tissue bone marrow mesenchymal stromal cells osteogenesis pathways |
| title | Which stem cells to choose for regenerative medicine application: Bone marrow and adipose tissue stromal stem cells – Similarities and differences |
| title_full | Which stem cells to choose for regenerative medicine application: Bone marrow and adipose tissue stromal stem cells – Similarities and differences |
| title_fullStr | Which stem cells to choose for regenerative medicine application: Bone marrow and adipose tissue stromal stem cells – Similarities and differences |
| title_full_unstemmed | Which stem cells to choose for regenerative medicine application: Bone marrow and adipose tissue stromal stem cells – Similarities and differences |
| title_short | Which stem cells to choose for regenerative medicine application: Bone marrow and adipose tissue stromal stem cells – Similarities and differences |
| title_sort | which stem cells to choose for regenerative medicine application bone marrow and adipose tissue stromal stem cells similarities and differences |
| topic | adipose tissue bone marrow mesenchymal stromal cells osteogenesis pathways |
| url | http://www.jnsmonline.org/article.asp?issn=2589-627X;year=2018;volume=1;issue=2;spage=48;epage=54;aulast=Alajez |
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