Characterization of slow-cycling cells in the mouse cochlear lateral wall.
Cochlear spiral ligament fibrocytes (SLFs) play essential roles in the physiology of hearing including ion recycling and the generation of endocochlear potential. In adult animals, SLFs can repopulate after damages, yet little is known about the characteristics of proliferating cells that support SL...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179293&type=printable |
| _version_ | 1826578736356524032 |
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| author | Yang Li Kotaro Watanabe Masato Fujioka Kaoru Ogawa |
| author_facet | Yang Li Kotaro Watanabe Masato Fujioka Kaoru Ogawa |
| author_sort | Yang Li |
| collection | DOAJ |
| description | Cochlear spiral ligament fibrocytes (SLFs) play essential roles in the physiology of hearing including ion recycling and the generation of endocochlear potential. In adult animals, SLFs can repopulate after damages, yet little is known about the characteristics of proliferating cells that support SLFs' self-renewal. Here we report in detail about the characteristics of cycling cells in the spiral ligament (SL). Fifteen P6 mice and six noise-exposed P28 mice were injected with 5-bromo-2'-deoxyuridine (BrdU) for 7 days and we chased BrdU retaining cells for as long as 60 days. Immunohistochemistry revealed that the BrdU positive IB4 (an endotherial marker) negative cells expressed an early SLF marker Pou3f4 but negative for cleaved-Caspase 3. Marker studies revealed that type 3 SLFs displayed significantly higher percentage of BrdU+ cells compared to other subtypes. Notably, the cells retained BrdU until P72, demonstrating they were dividing slowly. In the noise-damaged mice, in contrast to the loss of the other types, the number of type 3 SLFs did not altered and the BrdU incorporating- phosphorylated Histone H3 positive type 3 cells were increased from day 1 to 14 after noise exposure. Furthermore, the cells repopulating type 1 area, where the cells diminished profoundly after damage, were positive for the type 3 SLF markers. Collectively, in the latral wall of the cochlea, type 3 SLFs have the stem cell capacity and may contribute to the endogenous regeneration of lateral wall spiral ligament. Manipulating type 3 cells may be employed for potential regenerative therapies. |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T14:07:07Z |
| publishDate | 2017-01-01 |
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| series | PLoS ONE |
| spelling | doaj.art-0cf443d5c08c469398cad3957996a98f2025-02-27T05:38:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01126e017929310.1371/journal.pone.0179293Characterization of slow-cycling cells in the mouse cochlear lateral wall.Yang LiKotaro WatanabeMasato FujiokaKaoru OgawaCochlear spiral ligament fibrocytes (SLFs) play essential roles in the physiology of hearing including ion recycling and the generation of endocochlear potential. In adult animals, SLFs can repopulate after damages, yet little is known about the characteristics of proliferating cells that support SLFs' self-renewal. Here we report in detail about the characteristics of cycling cells in the spiral ligament (SL). Fifteen P6 mice and six noise-exposed P28 mice were injected with 5-bromo-2'-deoxyuridine (BrdU) for 7 days and we chased BrdU retaining cells for as long as 60 days. Immunohistochemistry revealed that the BrdU positive IB4 (an endotherial marker) negative cells expressed an early SLF marker Pou3f4 but negative for cleaved-Caspase 3. Marker studies revealed that type 3 SLFs displayed significantly higher percentage of BrdU+ cells compared to other subtypes. Notably, the cells retained BrdU until P72, demonstrating they were dividing slowly. In the noise-damaged mice, in contrast to the loss of the other types, the number of type 3 SLFs did not altered and the BrdU incorporating- phosphorylated Histone H3 positive type 3 cells were increased from day 1 to 14 after noise exposure. Furthermore, the cells repopulating type 1 area, where the cells diminished profoundly after damage, were positive for the type 3 SLF markers. Collectively, in the latral wall of the cochlea, type 3 SLFs have the stem cell capacity and may contribute to the endogenous regeneration of lateral wall spiral ligament. Manipulating type 3 cells may be employed for potential regenerative therapies.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179293&type=printable |
| spellingShingle | Yang Li Kotaro Watanabe Masato Fujioka Kaoru Ogawa Characterization of slow-cycling cells in the mouse cochlear lateral wall. PLoS ONE |
| title | Characterization of slow-cycling cells in the mouse cochlear lateral wall. |
| title_full | Characterization of slow-cycling cells in the mouse cochlear lateral wall. |
| title_fullStr | Characterization of slow-cycling cells in the mouse cochlear lateral wall. |
| title_full_unstemmed | Characterization of slow-cycling cells in the mouse cochlear lateral wall. |
| title_short | Characterization of slow-cycling cells in the mouse cochlear lateral wall. |
| title_sort | characterization of slow cycling cells in the mouse cochlear lateral wall |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179293&type=printable |
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