Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord
In vertebrates, neurotrophins and their receptors play a fundamental role in the central and peripheral nervous systems. Several studies reported that each neurotrophin/receptor signalling pathway can perform various functions during axon development, neuronal growth, and plasticity. Previous invest...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/13/10953 |
| _version_ | 1797591530207182848 |
|---|---|
| author | Pietro Cacialli Serena Ricci Maurizio Lazzari Liliana Milani Valeria Franceschini |
| author_facet | Pietro Cacialli Serena Ricci Maurizio Lazzari Liliana Milani Valeria Franceschini |
| author_sort | Pietro Cacialli |
| collection | DOAJ |
| description | In vertebrates, neurotrophins and their receptors play a fundamental role in the central and peripheral nervous systems. Several studies reported that each neurotrophin/receptor signalling pathway can perform various functions during axon development, neuronal growth, and plasticity. Previous investigations in some fish species have identified neurotrophins and their receptors in the spinal cord under physiological conditions and after injuries, highlighting their potential role during regeneration. In our study, for the first time, we used an excellent animal model, the zebrafish (<i>Danio rerio</i>), to compare the mRNA localization patterns of neurotrophins and receptors in the spinal cord. We quantified the levels of mRNA using qPCR, and identified the transcription pattern of each neurotrophin/receptor pathway via in situ hybridization. Our data show that <i>ngf/trka</i> are the most transcribed members in the adult zebrafish spinal cord. |
| first_indexed | 2024-03-11T01:38:44Z |
| format | Article |
| id | doaj.art-0cf7990f443147e7bb85f24a40913662 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:38:44Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0cf7990f443147e7bb85f24a409136622023-11-18T16:45:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124131095310.3390/ijms241310953Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal CordPietro Cacialli0Serena Ricci1Maurizio Lazzari2Liliana Milani3Valeria Franceschini4Department of Biological, Geological and Environmental Sciences, University of Bologna, 40126 Bologna, ItalyDepartment of Biological, Geological and Environmental Sciences, University of Bologna, 40126 Bologna, ItalyDepartment of Biological, Geological and Environmental Sciences, University of Bologna, 40126 Bologna, ItalyDepartment of Biological, Geological and Environmental Sciences, University of Bologna, 40126 Bologna, ItalyDepartment of Biological, Geological and Environmental Sciences, University of Bologna, 40126 Bologna, ItalyIn vertebrates, neurotrophins and their receptors play a fundamental role in the central and peripheral nervous systems. Several studies reported that each neurotrophin/receptor signalling pathway can perform various functions during axon development, neuronal growth, and plasticity. Previous investigations in some fish species have identified neurotrophins and their receptors in the spinal cord under physiological conditions and after injuries, highlighting their potential role during regeneration. In our study, for the first time, we used an excellent animal model, the zebrafish (<i>Danio rerio</i>), to compare the mRNA localization patterns of neurotrophins and receptors in the spinal cord. We quantified the levels of mRNA using qPCR, and identified the transcription pattern of each neurotrophin/receptor pathway via in situ hybridization. Our data show that <i>ngf/trka</i> are the most transcribed members in the adult zebrafish spinal cord.https://www.mdpi.com/1422-0067/24/13/10953zebrafishneurotrophinneurotrophin-receptor TRKspinal cordaquatic animal model |
| spellingShingle | Pietro Cacialli Serena Ricci Maurizio Lazzari Liliana Milani Valeria Franceschini Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord International Journal of Molecular Sciences zebrafish neurotrophin neurotrophin-receptor TRK spinal cord aquatic animal model |
| title | Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord |
| title_full | Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord |
| title_fullStr | Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord |
| title_full_unstemmed | Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord |
| title_short | Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord |
| title_sort | transcription pattern of neurotrophic factors and their receptors in adult zebrafish spinal cord |
| topic | zebrafish neurotrophin neurotrophin-receptor TRK spinal cord aquatic animal model |
| url | https://www.mdpi.com/1422-0067/24/13/10953 |
| work_keys_str_mv | AT pietrocacialli transcriptionpatternofneurotrophicfactorsandtheirreceptorsinadultzebrafishspinalcord AT serenaricci transcriptionpatternofneurotrophicfactorsandtheirreceptorsinadultzebrafishspinalcord AT mauriziolazzari transcriptionpatternofneurotrophicfactorsandtheirreceptorsinadultzebrafishspinalcord AT lilianamilani transcriptionpatternofneurotrophicfactorsandtheirreceptorsinadultzebrafishspinalcord AT valeriafranceschini transcriptionpatternofneurotrophicfactorsandtheirreceptorsinadultzebrafishspinalcord |