Affinity-selected heparan sulfate collagen device promotes periodontal regeneration in an intrabony defect model in Macaca fascicularis
Abstract It is challenging to regenerate periodontal tissues fully. We have previously reported a heparan sulfate variant with enhanced affinity for bone morphogenetic protein-2, termed HS3, that enhanced periodontal tissue regeneration in a rodent model. Here we seek to transition this work closer...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-07-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-38818-y |
| _version_ | 1797774196213809152 |
|---|---|
| author | Xiaoman Luo Chau Sang Lau Bach Quang Le Tuan Chun Tan Jian Hui Too Raymond Alexander Alfred Smith Na Yu Simon M. Cool |
| author_facet | Xiaoman Luo Chau Sang Lau Bach Quang Le Tuan Chun Tan Jian Hui Too Raymond Alexander Alfred Smith Na Yu Simon M. Cool |
| author_sort | Xiaoman Luo |
| collection | DOAJ |
| description | Abstract It is challenging to regenerate periodontal tissues fully. We have previously reported a heparan sulfate variant with enhanced affinity for bone morphogenetic protein-2, termed HS3, that enhanced periodontal tissue regeneration in a rodent model. Here we seek to transition this work closer to the clinic and investigate the efficacy of the combination HS3 collagen device in a non-human primate (NHP) periodontitis model. Wire-induced periodontitis was generated in ten Macaca fascicularis, and defects were treated with Emdogain or collagen (CollaPlug) loaded with (1) distilled water, (2) HS low (36 µg of HS3), or (3) HS high (180 µg of HS3) for 3 months. At the endpoint, microscopic assessment showed significantly less epithelial down-growth, greater alveolar bone filling, and enhanced cementum and periodontal ligament regeneration following treatment with the HS-collagen combination devices. When evaluated using a periodontal regeneration assessment score (PRAS) on a scale of 0–16, collagen scored 6.78 (± 2.64), Emdogain scored 10.50 (± 1.73) and HS low scored 10.40 (± 1.82). Notably, treatment with HS high scored 12.27 (± 2.20), while healthy control scored 14.80 (± 1.15). This study highlights the efficacy of an HS-collagen device for periodontal regeneration in a clinically relevant NHP periodontitis model and warrants its application in clinical trials. |
| first_indexed | 2024-03-12T22:17:29Z |
| format | Article |
| id | doaj.art-0cf7a89fd8db47a9b66a4c83dabf92fb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T22:17:29Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0cf7a89fd8db47a9b66a4c83dabf92fb2023-07-23T11:14:10ZengNature PortfolioScientific Reports2045-23222023-07-0113111510.1038/s41598-023-38818-yAffinity-selected heparan sulfate collagen device promotes periodontal regeneration in an intrabony defect model in Macaca fascicularisXiaoman Luo0Chau Sang Lau1Bach Quang Le2Tuan Chun Tan3Jian Hui Too4Raymond Alexander Alfred Smith5Na Yu6Simon M. Cool7Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR)National Dental Research Institute Singapore, National Dental Centre SingaporeBioprocessing Technology Institute, Agency for Science Technology and Research (A*STAR)Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR)National Dental Research Institute Singapore, National Dental Centre SingaporeInstitute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR)National Dental Research Institute Singapore, National Dental Centre SingaporeInstitute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR)Abstract It is challenging to regenerate periodontal tissues fully. We have previously reported a heparan sulfate variant with enhanced affinity for bone morphogenetic protein-2, termed HS3, that enhanced periodontal tissue regeneration in a rodent model. Here we seek to transition this work closer to the clinic and investigate the efficacy of the combination HS3 collagen device in a non-human primate (NHP) periodontitis model. Wire-induced periodontitis was generated in ten Macaca fascicularis, and defects were treated with Emdogain or collagen (CollaPlug) loaded with (1) distilled water, (2) HS low (36 µg of HS3), or (3) HS high (180 µg of HS3) for 3 months. At the endpoint, microscopic assessment showed significantly less epithelial down-growth, greater alveolar bone filling, and enhanced cementum and periodontal ligament regeneration following treatment with the HS-collagen combination devices. When evaluated using a periodontal regeneration assessment score (PRAS) on a scale of 0–16, collagen scored 6.78 (± 2.64), Emdogain scored 10.50 (± 1.73) and HS low scored 10.40 (± 1.82). Notably, treatment with HS high scored 12.27 (± 2.20), while healthy control scored 14.80 (± 1.15). This study highlights the efficacy of an HS-collagen device for periodontal regeneration in a clinically relevant NHP periodontitis model and warrants its application in clinical trials.https://doi.org/10.1038/s41598-023-38818-y |
| spellingShingle | Xiaoman Luo Chau Sang Lau Bach Quang Le Tuan Chun Tan Jian Hui Too Raymond Alexander Alfred Smith Na Yu Simon M. Cool Affinity-selected heparan sulfate collagen device promotes periodontal regeneration in an intrabony defect model in Macaca fascicularis Scientific Reports |
| title | Affinity-selected heparan sulfate collagen device promotes periodontal regeneration in an intrabony defect model in Macaca fascicularis |
| title_full | Affinity-selected heparan sulfate collagen device promotes periodontal regeneration in an intrabony defect model in Macaca fascicularis |
| title_fullStr | Affinity-selected heparan sulfate collagen device promotes periodontal regeneration in an intrabony defect model in Macaca fascicularis |
| title_full_unstemmed | Affinity-selected heparan sulfate collagen device promotes periodontal regeneration in an intrabony defect model in Macaca fascicularis |
| title_short | Affinity-selected heparan sulfate collagen device promotes periodontal regeneration in an intrabony defect model in Macaca fascicularis |
| title_sort | affinity selected heparan sulfate collagen device promotes periodontal regeneration in an intrabony defect model in macaca fascicularis |
| url | https://doi.org/10.1038/s41598-023-38818-y |
| work_keys_str_mv | AT xiaomanluo affinityselectedheparansulfatecollagendevicepromotesperiodontalregenerationinanintrabonydefectmodelinmacacafascicularis AT chausanglau affinityselectedheparansulfatecollagendevicepromotesperiodontalregenerationinanintrabonydefectmodelinmacacafascicularis AT bachquangle affinityselectedheparansulfatecollagendevicepromotesperiodontalregenerationinanintrabonydefectmodelinmacacafascicularis AT tuanchuntan affinityselectedheparansulfatecollagendevicepromotesperiodontalregenerationinanintrabonydefectmodelinmacacafascicularis AT jianhuitoo affinityselectedheparansulfatecollagendevicepromotesperiodontalregenerationinanintrabonydefectmodelinmacacafascicularis AT raymondalexanderalfredsmith affinityselectedheparansulfatecollagendevicepromotesperiodontalregenerationinanintrabonydefectmodelinmacacafascicularis AT nayu affinityselectedheparansulfatecollagendevicepromotesperiodontalregenerationinanintrabonydefectmodelinmacacafascicularis AT simonmcool affinityselectedheparansulfatecollagendevicepromotesperiodontalregenerationinanintrabonydefectmodelinmacacafascicularis |