Insights into melatonin-induced photosynthetic electron transport under low-temperature stress in cucumber
In this study, the differences in chlorophyll fluorescence transient (OJIP) and modulated 820 nm reflection (MR820) of cucumber leaves were probed to demonstrate an insight into the precise influence of melatonin (MT) on cucumber photosystems under low temperature stress. We pre-treated cucumber see...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-11-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1029854/full |
| _version_ | 1828100555497537536 |
|---|---|
| author | Pei Wu Pei Wu Yadong Ma Yadong Ma Golam Jalal Ahammed Baoyu Hao Baoyu Hao Jingyi Chen Jingyi Chen Wenliang Wan Wenliang Wan Yanhui Zhao Yanhui Zhao Huimei Cui Huimei Cui Wei Xu Wei Xu Jinxia Cui Jinxia Cui Huiying Liu Huiying Liu |
| author_facet | Pei Wu Pei Wu Yadong Ma Yadong Ma Golam Jalal Ahammed Baoyu Hao Baoyu Hao Jingyi Chen Jingyi Chen Wenliang Wan Wenliang Wan Yanhui Zhao Yanhui Zhao Huimei Cui Huimei Cui Wei Xu Wei Xu Jinxia Cui Jinxia Cui Huiying Liu Huiying Liu |
| author_sort | Pei Wu |
| collection | DOAJ |
| description | In this study, the differences in chlorophyll fluorescence transient (OJIP) and modulated 820 nm reflection (MR820) of cucumber leaves were probed to demonstrate an insight into the precise influence of melatonin (MT) on cucumber photosystems under low temperature stress. We pre-treated cucumber seedlings with different levels of MT (0, 25, 50, 100, 200, and 400 μmol · L-1) before imposing low temperature stress (10 °C/6 °C). The results indicated that moderate concentrations of MT had a positive effect on the growth of low temperature-stressed cucumber seedlings. Under low temperature stress conditions, 100 μmol · L-1 (MT 100) improved the performance of the active photosystem II (PSII) reaction centers (PIabs), the oxygen evolving complex activity (OEC centers) and electron transport between PSII and PSI, mainly by decreasing the L-band, K-band, and G-band, but showed differences with different duration of low temperature stress. In addition, these indicators related to quantum yield and energy flux of PSII regulated by MT indicated that MT (MT 100) effectively protected the electron transport and energy distribution in the photosystem. According to the results of WO-I ≥ 1 and MR820 signals, MT also affected PSI activity. MT 100 decreased the minimal value of MR/MRO and the oxidation rate of plastocyanin (PC) and PSI reaction center (P700) (Vox), while increased △MRslow/MRO and deoxidation rates of PC+ and P700+ (Vred). The loss of the slow phase of MT 200 and MT 400-treated plants in the MR820 kinetics was due to the complete prevention of electron movement from PSII to re-reduce the PC+ and P700 +. These results suggest that appropriate MT concentration (100 μmol · L-1) can improve the photosynthetic performance of PS II and electron transport from primary quinone electron acceptor (QA) to secondary quinone electron acceptor (QB), promote the balance of energy distribution, strengthen the connectivity of PSI and PSII, improve the electron flow of PSII via QA to PC+ and P700+ from reaching PSI by regulating multiple sites of electron transport chain in photosynthesis, and increase the pool size and reduction rates of PSI in low temperature-stressed cucumber plants, All these modifications by MT 100 treatment promoted the photosynthetic electron transfer smoothly, and further restored the cucumber plant growth under low temperature stress. Therefore, we conclude that spraying MT at an appropriate concentration is beneficial for protecting the photosynthetic electron transport chain, while spraying high concentrations of MT has a negative effect on regulating the low temperature tolerance in cucumber. |
| first_indexed | 2024-04-11T08:31:52Z |
| format | Article |
| id | doaj.art-0cf7ea27b85348a9bb277defddea90c4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T08:31:52Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-0cf7ea27b85348a9bb277defddea90c42022-12-22T04:34:27ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10298541029854Insights into melatonin-induced photosynthetic electron transport under low-temperature stress in cucumberPei Wu0Pei Wu1Yadong Ma2Yadong Ma3Golam Jalal Ahammed4Baoyu Hao5Baoyu Hao6Jingyi Chen7Jingyi Chen8Wenliang Wan9Wenliang Wan10Yanhui Zhao11Yanhui Zhao12Huimei Cui13Huimei Cui14Wei Xu15Wei Xu16Jinxia Cui17Jinxia Cui18Huiying Liu19Huiying Liu20Department of Horticulture, Agricultural College, Shihezi University, Shihezi, ChinaThe Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization in Xinjiang Production and Construction Group, Shihezi University, Shihezi, ChinaDepartment of Horticulture, Agricultural College, Shihezi University, Shihezi, ChinaThe Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization in Xinjiang Production and Construction Group, Shihezi University, Shihezi, ChinaCollege of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, ChinaDepartment of Horticulture, Agricultural College, Shihezi University, Shihezi, ChinaThe Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization in Xinjiang Production and Construction Group, Shihezi University, Shihezi, ChinaDepartment of Horticulture, Agricultural College, Shihezi University, Shihezi, ChinaThe Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization in Xinjiang Production and Construction Group, Shihezi University, Shihezi, ChinaDepartment of Horticulture, Agricultural College, Shihezi University, Shihezi, ChinaThe Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization in Xinjiang Production and Construction Group, Shihezi University, Shihezi, ChinaDepartment of Horticulture, Agricultural College, Shihezi University, Shihezi, ChinaThe Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization in Xinjiang Production and Construction Group, Shihezi University, Shihezi, ChinaDepartment of Horticulture, Agricultural College, Shihezi University, Shihezi, ChinaThe Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization in Xinjiang Production and Construction Group, Shihezi University, Shihezi, ChinaDepartment of Horticulture, Agricultural College, Shihezi University, Shihezi, ChinaThe Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization in Xinjiang Production and Construction Group, Shihezi University, Shihezi, ChinaDepartment of Horticulture, Agricultural College, Shihezi University, Shihezi, ChinaThe Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization in Xinjiang Production and Construction Group, Shihezi University, Shihezi, ChinaDepartment of Horticulture, Agricultural College, Shihezi University, Shihezi, ChinaThe Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization in Xinjiang Production and Construction Group, Shihezi University, Shihezi, ChinaIn this study, the differences in chlorophyll fluorescence transient (OJIP) and modulated 820 nm reflection (MR820) of cucumber leaves were probed to demonstrate an insight into the precise influence of melatonin (MT) on cucumber photosystems under low temperature stress. We pre-treated cucumber seedlings with different levels of MT (0, 25, 50, 100, 200, and 400 μmol · L-1) before imposing low temperature stress (10 °C/6 °C). The results indicated that moderate concentrations of MT had a positive effect on the growth of low temperature-stressed cucumber seedlings. Under low temperature stress conditions, 100 μmol · L-1 (MT 100) improved the performance of the active photosystem II (PSII) reaction centers (PIabs), the oxygen evolving complex activity (OEC centers) and electron transport between PSII and PSI, mainly by decreasing the L-band, K-band, and G-band, but showed differences with different duration of low temperature stress. In addition, these indicators related to quantum yield and energy flux of PSII regulated by MT indicated that MT (MT 100) effectively protected the electron transport and energy distribution in the photosystem. According to the results of WO-I ≥ 1 and MR820 signals, MT also affected PSI activity. MT 100 decreased the minimal value of MR/MRO and the oxidation rate of plastocyanin (PC) and PSI reaction center (P700) (Vox), while increased △MRslow/MRO and deoxidation rates of PC+ and P700+ (Vred). The loss of the slow phase of MT 200 and MT 400-treated plants in the MR820 kinetics was due to the complete prevention of electron movement from PSII to re-reduce the PC+ and P700 +. These results suggest that appropriate MT concentration (100 μmol · L-1) can improve the photosynthetic performance of PS II and electron transport from primary quinone electron acceptor (QA) to secondary quinone electron acceptor (QB), promote the balance of energy distribution, strengthen the connectivity of PSI and PSII, improve the electron flow of PSII via QA to PC+ and P700+ from reaching PSI by regulating multiple sites of electron transport chain in photosynthesis, and increase the pool size and reduction rates of PSI in low temperature-stressed cucumber plants, All these modifications by MT 100 treatment promoted the photosynthetic electron transfer smoothly, and further restored the cucumber plant growth under low temperature stress. Therefore, we conclude that spraying MT at an appropriate concentration is beneficial for protecting the photosynthetic electron transport chain, while spraying high concentrations of MT has a negative effect on regulating the low temperature tolerance in cucumber.https://www.frontiersin.org/articles/10.3389/fpls.2022.1029854/fullcucumberlow temperaturemelatoninOJIPMR820 signalJIP-test |
| spellingShingle | Pei Wu Pei Wu Yadong Ma Yadong Ma Golam Jalal Ahammed Baoyu Hao Baoyu Hao Jingyi Chen Jingyi Chen Wenliang Wan Wenliang Wan Yanhui Zhao Yanhui Zhao Huimei Cui Huimei Cui Wei Xu Wei Xu Jinxia Cui Jinxia Cui Huiying Liu Huiying Liu Insights into melatonin-induced photosynthetic electron transport under low-temperature stress in cucumber Frontiers in Plant Science cucumber low temperature melatonin OJIP MR820 signal JIP-test |
| title | Insights into melatonin-induced photosynthetic electron transport under low-temperature stress in cucumber |
| title_full | Insights into melatonin-induced photosynthetic electron transport under low-temperature stress in cucumber |
| title_fullStr | Insights into melatonin-induced photosynthetic electron transport under low-temperature stress in cucumber |
| title_full_unstemmed | Insights into melatonin-induced photosynthetic electron transport under low-temperature stress in cucumber |
| title_short | Insights into melatonin-induced photosynthetic electron transport under low-temperature stress in cucumber |
| title_sort | insights into melatonin induced photosynthetic electron transport under low temperature stress in cucumber |
| topic | cucumber low temperature melatonin OJIP MR820 signal JIP-test |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1029854/full |
| work_keys_str_mv | AT peiwu insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT peiwu insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT yadongma insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT yadongma insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT golamjalalahammed insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT baoyuhao insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT baoyuhao insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT jingyichen insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT jingyichen insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT wenliangwan insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT wenliangwan insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT yanhuizhao insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT yanhuizhao insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT huimeicui insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT huimeicui insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT weixu insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT weixu insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT jinxiacui insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT jinxiacui insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT huiyingliu insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber AT huiyingliu insightsintomelatonininducedphotosyntheticelectrontransportunderlowtemperaturestressincucumber |