Saccharomyces boulardii promoters for control of gene expression in vivo
Abstract Background Interest in the use of engineered microbes to deliver therapeutic activities has increased in recent years. The probiotic yeast Saccharomyces boulardii has been investigated for production of therapeutics in the gastrointestinal tract. Well-characterised promoters are a prerequis...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Microbial Cell Factories |
| Online Access: | https://doi.org/10.1186/s12934-023-02288-8 |
| _version_ | 1797362687457361920 |
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| author | Carmen Sands Karl Alex Hedin Ruben Vazquez-Uribe Morten Otto Alexander Sommer |
| author_facet | Carmen Sands Karl Alex Hedin Ruben Vazquez-Uribe Morten Otto Alexander Sommer |
| author_sort | Carmen Sands |
| collection | DOAJ |
| description | Abstract Background Interest in the use of engineered microbes to deliver therapeutic activities has increased in recent years. The probiotic yeast Saccharomyces boulardii has been investigated for production of therapeutics in the gastrointestinal tract. Well-characterised promoters are a prerequisite for robust therapeutic expression in the gut; however, S. boulardii promoters have not yet been thoroughly characterised in vitro and in vivo. Results We present a thorough characterisation of the expression activities of 12 S. boulardii promoters in vitro in glucose, fructose, sucrose, inulin and acetate, under both aerobic and anaerobic conditions, as well as in the murine gastrointestinal tract. Green fluorescent protein was used to report on promoter activity. Promoter expression was found to be carbon-source dependent, with inulin emerging as a favourable carbon source. Furthermore, relative promoter expression in vivo was highly correlated with expression in sucrose (R = 0.99). Conclusions These findings provide insights into S. boulardii promoter activity and aid in promoter selection in future studies utilising S. boulardii to produce therapeutics in the gut. |
| first_indexed | 2024-03-08T16:11:01Z |
| format | Article |
| id | doaj.art-0cf96817042447be8c1590fca0d75c92 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-03-08T16:11:01Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-0cf96817042447be8c1590fca0d75c922024-01-07T12:55:44ZengBMCMicrobial Cell Factories1475-28592024-01-0123111210.1186/s12934-023-02288-8Saccharomyces boulardii promoters for control of gene expression in vivoCarmen Sands0Karl Alex Hedin1Ruben Vazquez-Uribe2Morten Otto Alexander Sommer3Novo Nordisk Foundation Center for Biosustainability, Technical University of DenmarkNovo Nordisk Foundation Center for Biosustainability, Technical University of DenmarkNovo Nordisk Foundation Center for Biosustainability, Technical University of DenmarkNovo Nordisk Foundation Center for Biosustainability, Technical University of DenmarkAbstract Background Interest in the use of engineered microbes to deliver therapeutic activities has increased in recent years. The probiotic yeast Saccharomyces boulardii has been investigated for production of therapeutics in the gastrointestinal tract. Well-characterised promoters are a prerequisite for robust therapeutic expression in the gut; however, S. boulardii promoters have not yet been thoroughly characterised in vitro and in vivo. Results We present a thorough characterisation of the expression activities of 12 S. boulardii promoters in vitro in glucose, fructose, sucrose, inulin and acetate, under both aerobic and anaerobic conditions, as well as in the murine gastrointestinal tract. Green fluorescent protein was used to report on promoter activity. Promoter expression was found to be carbon-source dependent, with inulin emerging as a favourable carbon source. Furthermore, relative promoter expression in vivo was highly correlated with expression in sucrose (R = 0.99). Conclusions These findings provide insights into S. boulardii promoter activity and aid in promoter selection in future studies utilising S. boulardii to produce therapeutics in the gut.https://doi.org/10.1186/s12934-023-02288-8 |
| spellingShingle | Carmen Sands Karl Alex Hedin Ruben Vazquez-Uribe Morten Otto Alexander Sommer Saccharomyces boulardii promoters for control of gene expression in vivo Microbial Cell Factories |
| title | Saccharomyces boulardii promoters for control of gene expression in vivo |
| title_full | Saccharomyces boulardii promoters for control of gene expression in vivo |
| title_fullStr | Saccharomyces boulardii promoters for control of gene expression in vivo |
| title_full_unstemmed | Saccharomyces boulardii promoters for control of gene expression in vivo |
| title_short | Saccharomyces boulardii promoters for control of gene expression in vivo |
| title_sort | saccharomyces boulardii promoters for control of gene expression in vivo |
| url | https://doi.org/10.1186/s12934-023-02288-8 |
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