Immunohistochemical Study of Lymph Nodes in Patients with Cat Scratch Disease
Bartonella henselae is the causative agent of cat scratch disease (CSD), manifesting as fever and acute regional lymphadenopathy. Although serologic testing is the reference method for diagnosis, successful use of immunohistochemical (IHC) stain of regional lymph nodes for the diagnosis of CSD has b...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2006-01-01
|
| Series: | Journal of the Formosan Medical Association |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664609601765 |
| _version_ | 1818340935595982848 |
|---|---|
| author | Ying-You Lin Cheng-Hsiang Hsiao Yung-Hsiang Hsu Chin-Cheng Lee Hsiang-Jung Tsai Ming-Jeng Pan |
| author_facet | Ying-You Lin Cheng-Hsiang Hsiao Yung-Hsiang Hsu Chin-Cheng Lee Hsiang-Jung Tsai Ming-Jeng Pan |
| author_sort | Ying-You Lin |
| collection | DOAJ |
| description | Bartonella henselae is the causative agent of cat scratch disease (CSD), manifesting as fever and acute regional lymphadenopathy. Although serologic testing is the reference method for diagnosis, successful use of immunohistochemical (IHC) stain of regional lymph nodes for the diagnosis of CSD has been reported. To determine the characterization and diagnostic potential of IHC in lymphadenopathy of CSD, lymph nodes were excised from patients with suspected CSD for further evaluation.
Methods: Polyclonal antibody-based IHC studies were performed for the detection of B. henselae. Between January 2001 and December 2004, the reference laboratory of the Center for Disease Control, Taiwan, received a total of 377 sera from 352 reported suspected CSD cases. Twenty-three formalin-fixed paraffin-embedded lymph nodes from 16 patients and two skin biopsies from two patients suspected of having CSD were included in this study. Nine of them were serologically confirmed to have CSD and the others were seronegative but suspected to have CSD by the attending physicians. Seven lymph node specimens were obtained from tuberculosis patients for comparison.
Results: We demonstrated that the microorganisms existed in the cytoplasm of histiocytes within the granulomatous lesions in nine lymph nodes and one skin biopsy. Among the nine lymph nodes with IHC(+) stains, three were seronegative. On the other hand, three cases were IHC(+) and six cases were IHC(−) among nine seronegative patients. In addition, two seronegative patients with skin biopsy showed one IHC(+) and one IHC(−).
Conclusion: IHC can contribute to the etiologic diagnosis of B. henselae lymphadenopathy when serology and molecular techniques are not available. |
| first_indexed | 2024-12-13T15:50:48Z |
| format | Article |
| id | doaj.art-0cfcf6da5fba4f9494643c2c28d11d29 |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-13T15:50:48Z |
| publishDate | 2006-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-0cfcf6da5fba4f9494643c2c28d11d292022-12-21T23:39:31ZengElsevierJournal of the Formosan Medical Association0929-66462006-01-011051191191710.1016/S0929-6646(09)60176-5Immunohistochemical Study of Lymph Nodes in Patients with Cat Scratch DiseaseYing-You Lin0Cheng-Hsiang Hsiao1Yung-Hsiang Hsu2Chin-Cheng Lee3Hsiang-Jung Tsai4Ming-Jeng Pan5Graduate Institute of Veterinary Medicine, National Taiwan University, TaiwanDepartment of Pathology, National Taiwan University Hospital, Taipei, TaiwanBuddhist Tzu Chi General Hospital, Hualien, TaiwanShin Kong Wu Ho-Su Memorial Hospital, Taipei, TaiwanGraduate Institute of Veterinary Medicine, National Taiwan University, TaiwanGraduate Institute of Veterinary Medicine, National Taiwan University, TaiwanBartonella henselae is the causative agent of cat scratch disease (CSD), manifesting as fever and acute regional lymphadenopathy. Although serologic testing is the reference method for diagnosis, successful use of immunohistochemical (IHC) stain of regional lymph nodes for the diagnosis of CSD has been reported. To determine the characterization and diagnostic potential of IHC in lymphadenopathy of CSD, lymph nodes were excised from patients with suspected CSD for further evaluation. Methods: Polyclonal antibody-based IHC studies were performed for the detection of B. henselae. Between January 2001 and December 2004, the reference laboratory of the Center for Disease Control, Taiwan, received a total of 377 sera from 352 reported suspected CSD cases. Twenty-three formalin-fixed paraffin-embedded lymph nodes from 16 patients and two skin biopsies from two patients suspected of having CSD were included in this study. Nine of them were serologically confirmed to have CSD and the others were seronegative but suspected to have CSD by the attending physicians. Seven lymph node specimens were obtained from tuberculosis patients for comparison. Results: We demonstrated that the microorganisms existed in the cytoplasm of histiocytes within the granulomatous lesions in nine lymph nodes and one skin biopsy. Among the nine lymph nodes with IHC(+) stains, three were seronegative. On the other hand, three cases were IHC(+) and six cases were IHC(−) among nine seronegative patients. In addition, two seronegative patients with skin biopsy showed one IHC(+) and one IHC(−). Conclusion: IHC can contribute to the etiologic diagnosis of B. henselae lymphadenopathy when serology and molecular techniques are not available.http://www.sciencedirect.com/science/article/pii/S0929664609601765Bartonella henselaecat scratch diseaseimmunohistochemistrylymph nodes |
| spellingShingle | Ying-You Lin Cheng-Hsiang Hsiao Yung-Hsiang Hsu Chin-Cheng Lee Hsiang-Jung Tsai Ming-Jeng Pan Immunohistochemical Study of Lymph Nodes in Patients with Cat Scratch Disease Journal of the Formosan Medical Association Bartonella henselae cat scratch disease immunohistochemistry lymph nodes |
| title | Immunohistochemical Study of Lymph Nodes in Patients with Cat Scratch Disease |
| title_full | Immunohistochemical Study of Lymph Nodes in Patients with Cat Scratch Disease |
| title_fullStr | Immunohistochemical Study of Lymph Nodes in Patients with Cat Scratch Disease |
| title_full_unstemmed | Immunohistochemical Study of Lymph Nodes in Patients with Cat Scratch Disease |
| title_short | Immunohistochemical Study of Lymph Nodes in Patients with Cat Scratch Disease |
| title_sort | immunohistochemical study of lymph nodes in patients with cat scratch disease |
| topic | Bartonella henselae cat scratch disease immunohistochemistry lymph nodes |
| url | http://www.sciencedirect.com/science/article/pii/S0929664609601765 |
| work_keys_str_mv | AT yingyoulin immunohistochemicalstudyoflymphnodesinpatientswithcatscratchdisease AT chenghsianghsiao immunohistochemicalstudyoflymphnodesinpatientswithcatscratchdisease AT yunghsianghsu immunohistochemicalstudyoflymphnodesinpatientswithcatscratchdisease AT chinchenglee immunohistochemicalstudyoflymphnodesinpatientswithcatscratchdisease AT hsiangjungtsai immunohistochemicalstudyoflymphnodesinpatientswithcatscratchdisease AT mingjengpan immunohistochemicalstudyoflymphnodesinpatientswithcatscratchdisease |