Optimizing the sensing performance of amperometric creatinine detection based on creatinine deiminase/Nafion®-nanostructured polyaniline composite film by mixture design method
Nafion®-nanostructured polyaniline (nsPANi) composite film is prepared using cyclic voltammetry (CV) and immobilized with creatinine deiminase (CD) enzyme and is used to sense creatinine in a buffer phosphate solution. The conditions for preparing Nafion®-nsPANi composite film are optimized by using...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Sensors and Actuators Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666053922000625 |
| _version_ | 1797802695106494464 |
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| author | Jing-Shan Do Yu-Hsuan Chang |
| author_facet | Jing-Shan Do Yu-Hsuan Chang |
| author_sort | Jing-Shan Do |
| collection | DOAJ |
| description | Nafion®-nanostructured polyaniline (nsPANi) composite film is prepared using cyclic voltammetry (CV) and immobilized with creatinine deiminase (CD) enzyme and is used to sense creatinine in a buffer phosphate solution. The conditions for preparing Nafion®-nsPANi composite film are optimized by using a mixture design for which the sensitivity is the response. The relationship between the sensitivity of the amperometric creatinine biosensor (y) and the normalized aniline concentration (Y1), HCl concentration (Y2) and scanning rate (Y3) is y = 119.44Y1 + 45.23Y2 + 100.93Y3 + 255.69Y1Y2 + 313.16Y1Y3 + 430.56Y1Y2Y3The maximum sensitivity of an amperometric creatinine biosensor that is constructed using Nafion®-nsPANi composite film in 0.0943 M aniline, 0.9024 M HCl and using a scanning rate of 27.88 mV s − 1 is 2013.2 μA mM−1 cm−2, which is 54.9% better than the sensitivity of a conventional experimental technique. The amperometric creatinine biosensor is 6.60% less sensitive after sensing 0.15 mM creatinine 240 times. The amperometric creatinine biosensor incurs insignificant interference in 0.138 mM urea, 0.085 mM ascorbic acid (AA) and 5.54 mM glucose. |
| first_indexed | 2024-03-13T05:09:35Z |
| format | Article |
| id | doaj.art-0cfe1ea62b8c47029382c3ca9c3173fe |
| institution | Directory Open Access Journal |
| issn | 2666-0539 |
| language | English |
| last_indexed | 2024-03-13T05:09:35Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensors and Actuators Reports |
| spelling | doaj.art-0cfe1ea62b8c47029382c3ca9c3173fe2023-06-16T05:11:39ZengElsevierSensors and Actuators Reports2666-05392023-06-015100135Optimizing the sensing performance of amperometric creatinine detection based on creatinine deiminase/Nafion®-nanostructured polyaniline composite film by mixture design methodJing-Shan Do0Yu-Hsuan Chang1Corresponding author.; Department of Chemical and Materials Engineering, National Chin-Yi University of Technology, Taichung, ChinaDepartment of Chemical and Materials Engineering, National Chin-Yi University of Technology, Taichung, ChinaNafion®-nanostructured polyaniline (nsPANi) composite film is prepared using cyclic voltammetry (CV) and immobilized with creatinine deiminase (CD) enzyme and is used to sense creatinine in a buffer phosphate solution. The conditions for preparing Nafion®-nsPANi composite film are optimized by using a mixture design for which the sensitivity is the response. The relationship between the sensitivity of the amperometric creatinine biosensor (y) and the normalized aniline concentration (Y1), HCl concentration (Y2) and scanning rate (Y3) is y = 119.44Y1 + 45.23Y2 + 100.93Y3 + 255.69Y1Y2 + 313.16Y1Y3 + 430.56Y1Y2Y3The maximum sensitivity of an amperometric creatinine biosensor that is constructed using Nafion®-nsPANi composite film in 0.0943 M aniline, 0.9024 M HCl and using a scanning rate of 27.88 mV s − 1 is 2013.2 μA mM−1 cm−2, which is 54.9% better than the sensitivity of a conventional experimental technique. The amperometric creatinine biosensor is 6.60% less sensitive after sensing 0.15 mM creatinine 240 times. The amperometric creatinine biosensor incurs insignificant interference in 0.138 mM urea, 0.085 mM ascorbic acid (AA) and 5.54 mM glucose.http://www.sciencedirect.com/science/article/pii/S2666053922000625CreatinineAmperometric biosensorNafion®-nanostructured polyaniline composite filmMixture designOptimization |
| spellingShingle | Jing-Shan Do Yu-Hsuan Chang Optimizing the sensing performance of amperometric creatinine detection based on creatinine deiminase/Nafion®-nanostructured polyaniline composite film by mixture design method Sensors and Actuators Reports Creatinine Amperometric biosensor Nafion®-nanostructured polyaniline composite film Mixture design Optimization |
| title | Optimizing the sensing performance of amperometric creatinine detection based on creatinine deiminase/Nafion®-nanostructured polyaniline composite film by mixture design method |
| title_full | Optimizing the sensing performance of amperometric creatinine detection based on creatinine deiminase/Nafion®-nanostructured polyaniline composite film by mixture design method |
| title_fullStr | Optimizing the sensing performance of amperometric creatinine detection based on creatinine deiminase/Nafion®-nanostructured polyaniline composite film by mixture design method |
| title_full_unstemmed | Optimizing the sensing performance of amperometric creatinine detection based on creatinine deiminase/Nafion®-nanostructured polyaniline composite film by mixture design method |
| title_short | Optimizing the sensing performance of amperometric creatinine detection based on creatinine deiminase/Nafion®-nanostructured polyaniline composite film by mixture design method |
| title_sort | optimizing the sensing performance of amperometric creatinine detection based on creatinine deiminase nafion r nanostructured polyaniline composite film by mixture design method |
| topic | Creatinine Amperometric biosensor Nafion®-nanostructured polyaniline composite film Mixture design Optimization |
| url | http://www.sciencedirect.com/science/article/pii/S2666053922000625 |
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