A Dual-Function “TRE-Lox” System for Genetic Deletion or Reversible, Titratable, and Near-Complete Downregulation of Cathepsin D

A Dual-Function “TRE-Lox” System for Genetic Deletion or Reversible, Titratable, and Near-Complete Downregulation of Cathepsin D

Commonly employed methods for reversibly disrupting gene expression, such as those based on RNAi or CRISPRi, are rarely capable of achieving >80–90% downregulation, making them unsuitable for targeting genes that require more complete disruption to elicit a phenotype. Genetic deletion, on the oth...

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Bibliographic Details
Main Authors: Heather M. Terron, Derek S. Maranan, Luke A. Burgard, Frank M. LaFerla, Shelley Lane, Malcolm A. Leissring
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:International Journal of Molecular Sciences
Subjects:
Alzheimer disease
cathepsin D
Cre-Lox technology
CRISPR
gene regulation
tetracycline regulatory element
Online Access:https://www.mdpi.com/1422-0067/24/7/6745

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