Host-Dependent Requirement for the Potato leafroll virus 17-kDa Protein in Virus Movement
The requirement for the 17-kDa protein (P17) of Potato leafroll virus (PLRV) in virus movement was investigated in four plant species: potato (Solanum tuberosum), Physalis floridana, Nicotiana benthamiana, and N. clevelandii. Two PLRV P17 mutants were characterized, one that does not translate the P...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2002-10-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.10.1086 |
| _version_ | 1811264370634653696 |
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| author | Lawrence Lee Peter Palukaitis Stewart M. Gray |
| author_facet | Lawrence Lee Peter Palukaitis Stewart M. Gray |
| author_sort | Lawrence Lee |
| collection | DOAJ |
| description | The requirement for the 17-kDa protein (P17) of Potato leafroll virus (PLRV) in virus movement was investigated in four plant species: potato (Solanum tuberosum), Physalis floridana, Nicotiana benthamiana, and N. clevelandii. Two PLRV P17 mutants were characterized, one that does not translate the P17 and another that expresses a P17 missing the first four amino acids. The P17 mutants were able to replicate and accumulate in agroinoculated leaves of potato and P. floridana, but they were unable to move into vascular tissues and initiate a systemic infection in these plants. In contrast, the P17 mutants were able to spread systemically from inoculated leaves in both Nicotiana spp., although the efficiency of infection was reduced relative to wild-type PLRV. Examination of virus distribution in N. benthamiana plants using tissue immunoblotting techniques revealed that the wild-type PLRV and P17 mutants followed a similar movement pathway out of the inoculated leaves. Virus first moved upward to the apical tissues and then downward. The P17 mutants, however, infected fewer phloem-associated cells, were slower than wild-type PLRV in moving out of the inoculated tissue and into apical tissues, and were unable to infect any mature leaves present on the plant at the time of inoculation. |
| first_indexed | 2024-04-12T20:03:06Z |
| format | Article |
| id | doaj.art-0d06398dc93c4d32bcbc9e457d8fc817 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T20:03:06Z |
| publishDate | 2002-10-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-0d06398dc93c4d32bcbc9e457d8fc8172022-12-22T03:18:28ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062002-10-0115101086109410.1094/MPMI.2002.15.10.1086Host-Dependent Requirement for the Potato leafroll virus 17-kDa Protein in Virus MovementLawrence LeePeter PalukaitisStewart M. GrayThe requirement for the 17-kDa protein (P17) of Potato leafroll virus (PLRV) in virus movement was investigated in four plant species: potato (Solanum tuberosum), Physalis floridana, Nicotiana benthamiana, and N. clevelandii. Two PLRV P17 mutants were characterized, one that does not translate the P17 and another that expresses a P17 missing the first four amino acids. The P17 mutants were able to replicate and accumulate in agroinoculated leaves of potato and P. floridana, but they were unable to move into vascular tissues and initiate a systemic infection in these plants. In contrast, the P17 mutants were able to spread systemically from inoculated leaves in both Nicotiana spp., although the efficiency of infection was reduced relative to wild-type PLRV. Examination of virus distribution in N. benthamiana plants using tissue immunoblotting techniques revealed that the wild-type PLRV and P17 mutants followed a similar movement pathway out of the inoculated leaves. Virus first moved upward to the apical tissues and then downward. The P17 mutants, however, infected fewer phloem-associated cells, were slower than wild-type PLRV in moving out of the inoculated tissue and into apical tissues, and were unable to infect any mature leaves present on the plant at the time of inoculation.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.10.1086agroinoculationLuteoviridaemovement proteinPolerovirustissue immunoblot analysis |
| spellingShingle | Lawrence Lee Peter Palukaitis Stewart M. Gray Host-Dependent Requirement for the Potato leafroll virus 17-kDa Protein in Virus Movement Molecular Plant-Microbe Interactions agroinoculation Luteoviridae movement protein Polerovirus tissue immunoblot analysis |
| title | Host-Dependent Requirement for the Potato leafroll virus 17-kDa Protein in Virus Movement |
| title_full | Host-Dependent Requirement for the Potato leafroll virus 17-kDa Protein in Virus Movement |
| title_fullStr | Host-Dependent Requirement for the Potato leafroll virus 17-kDa Protein in Virus Movement |
| title_full_unstemmed | Host-Dependent Requirement for the Potato leafroll virus 17-kDa Protein in Virus Movement |
| title_short | Host-Dependent Requirement for the Potato leafroll virus 17-kDa Protein in Virus Movement |
| title_sort | host dependent requirement for the potato leafroll virus 17 kda protein in virus movement |
| topic | agroinoculation Luteoviridae movement protein Polerovirus tissue immunoblot analysis |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.10.1086 |
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