Identification of genes involved in regulating the development of feathered feet in chicken embryo
ABSTRACT: The genetic and developmental factors driving the diverse distribution and morphogenesis of feathers and scales on bird feet are yet unclear. Within a single species, Guangxi domestic chickens exhibit dramatic variety in feathered feet, making them an accessible model for research into the...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123003565 |
| _version_ | 1797738975354421248 |
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| author | Wenya Du Zhuliang Yang Cong Xiao Yongcui Liu Jiashuo Peng Jianneng Li Fuqiu Li Xiurong Yang |
| author_facet | Wenya Du Zhuliang Yang Cong Xiao Yongcui Liu Jiashuo Peng Jianneng Li Fuqiu Li Xiurong Yang |
| author_sort | Wenya Du |
| collection | DOAJ |
| description | ABSTRACT: The genetic and developmental factors driving the diverse distribution and morphogenesis of feathers and scales on bird feet are yet unclear. Within a single species, Guangxi domestic chickens exhibit dramatic variety in feathered feet, making them an accessible model for research into the molecular basis of variations in skin appendages. In this study, we used H&E staining to observe the morphogenesis of feathered feet, scaled feet and wings skin at different embryonic stages in Longsheng-Feng chickens and Guangxi Partridge chickens. We selected 4 periods (E6, E7, E8, and E12) that play an important role in feather development and performed transcriptome sequencing to screen for candidate genes associated with feathered feet. Through comparison and analysis of transcriptome data, we identified a set of differently expressed genes (DGEs), which were enriched in appendage organ development, hindlimb morphogenesis, activation of transcription factor binding, and binding of sequence-specific DNA in the cis-regulatory region. In addition, we identified some feathered feet-related genes by analyzing the classical signaling pathways that regulate feather development. Finally, we identified candidate genes that regulate feathered feet formation, which include TBX5, PITX1, ZIC1, FGF20, WNT11, WNT7A, WNT16, and SHH. Interestingly, we found that TBX5 was significantly overexpressed in the skin of the feathered feet and had the highest expression at E7 (P < 0.01), whereas PITX1 expression was significantly reduced at E7(P < 0.01). It is hypothesized that TBX5 and PITX1 regulate the development of hair follicles through the Wnt/β-catenin signaling pathway at E7. Our results provide a theoretical basis for investigating the molecular regulatory mechanisms underlying the formation of chicken feathered feet. |
| first_indexed | 2024-03-12T13:50:37Z |
| format | Article |
| id | doaj.art-0d0b1cb2c5954afda5dd87fd8714ff38 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-12T13:50:37Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-0d0b1cb2c5954afda5dd87fd8714ff382023-08-23T04:32:33ZengElsevierPoultry Science0032-57912023-09-011029102837Identification of genes involved in regulating the development of feathered feet in chicken embryoWenya Du0Zhuliang Yang1Cong Xiao2Yongcui Liu3Jiashuo Peng4Jianneng Li5Fuqiu Li6Xiurong Yang7College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaGuangxi Gangfeng Agriculture & Animal Husbandry Co., Ltd, Guigang 537000, ChinaGuangxi Gangfeng Agriculture & Animal Husbandry Co., Ltd, Guigang 537000, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China; Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning 530004, China; Corresponding author:ABSTRACT: The genetic and developmental factors driving the diverse distribution and morphogenesis of feathers and scales on bird feet are yet unclear. Within a single species, Guangxi domestic chickens exhibit dramatic variety in feathered feet, making them an accessible model for research into the molecular basis of variations in skin appendages. In this study, we used H&E staining to observe the morphogenesis of feathered feet, scaled feet and wings skin at different embryonic stages in Longsheng-Feng chickens and Guangxi Partridge chickens. We selected 4 periods (E6, E7, E8, and E12) that play an important role in feather development and performed transcriptome sequencing to screen for candidate genes associated with feathered feet. Through comparison and analysis of transcriptome data, we identified a set of differently expressed genes (DGEs), which were enriched in appendage organ development, hindlimb morphogenesis, activation of transcription factor binding, and binding of sequence-specific DNA in the cis-regulatory region. In addition, we identified some feathered feet-related genes by analyzing the classical signaling pathways that regulate feather development. Finally, we identified candidate genes that regulate feathered feet formation, which include TBX5, PITX1, ZIC1, FGF20, WNT11, WNT7A, WNT16, and SHH. Interestingly, we found that TBX5 was significantly overexpressed in the skin of the feathered feet and had the highest expression at E7 (P < 0.01), whereas PITX1 expression was significantly reduced at E7(P < 0.01). It is hypothesized that TBX5 and PITX1 regulate the development of hair follicles through the Wnt/β-catenin signaling pathway at E7. Our results provide a theoretical basis for investigating the molecular regulatory mechanisms underlying the formation of chicken feathered feet.http://www.sciencedirect.com/science/article/pii/S0032579123003565chickenfeathered feetRNA-seqTBX5PITX1signaling pathway |
| spellingShingle | Wenya Du Zhuliang Yang Cong Xiao Yongcui Liu Jiashuo Peng Jianneng Li Fuqiu Li Xiurong Yang Identification of genes involved in regulating the development of feathered feet in chicken embryo Poultry Science chicken feathered feet RNA-seq TBX5 PITX1 signaling pathway |
| title | Identification of genes involved in regulating the development of feathered feet in chicken embryo |
| title_full | Identification of genes involved in regulating the development of feathered feet in chicken embryo |
| title_fullStr | Identification of genes involved in regulating the development of feathered feet in chicken embryo |
| title_full_unstemmed | Identification of genes involved in regulating the development of feathered feet in chicken embryo |
| title_short | Identification of genes involved in regulating the development of feathered feet in chicken embryo |
| title_sort | identification of genes involved in regulating the development of feathered feet in chicken embryo |
| topic | chicken feathered feet RNA-seq TBX5 PITX1 signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123003565 |
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