Electroactive Tissue Scaffolds with Aligned Pores as Instructive Platforms for Biomimetic Tissue Engineering
Tissues in the body are hierarchically structured composite materials with tissue-specific chemical and topographical properties. Here we report the preparation of tissue scaffolds with macroscopic pores generated via the dissolution of a sacrificial supramolecular polymer-based crystal template (u...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-01-01
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| Series: | Bioengineering |
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| Online Access: | http://www.mdpi.com/2306-5354/2/1/15 |
| _version_ | 1797711692493225984 |
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| author | John G. Hardy R. Chase Cornelison Rushi C. Sukhavasi Richard J. Saballos Philip Vu David L. Kaplan Christine E. Schmidt |
| author_facet | John G. Hardy R. Chase Cornelison Rushi C. Sukhavasi Richard J. Saballos Philip Vu David L. Kaplan Christine E. Schmidt |
| author_sort | John G. Hardy |
| collection | DOAJ |
| description | Tissues in the body are hierarchically structured composite materials with tissue-specific chemical and topographical properties. Here we report the preparation of tissue scaffolds with macroscopic pores generated via the dissolution of a sacrificial supramolecular polymer-based crystal template (urea) from a biodegradable polymer-based scaffold (polycaprolactone, PCL). Furthermore, we report a method of aligning the supramolecular polymer-based crystals within the PCL, and that the dissolution of the sacrificial urea yields scaffolds with macroscopic pores that are aligned over long, clinically-relevant distances (i.e., centimeter scale). The pores act as topographical cues to which rat Schwann cells respond by aligning with the long axis of the pores. Generation of an interpenetrating network of polypyrrole (PPy) and poly(styrene sulfonate) (PSS) in the scaffolds yields electroactive tissue scaffolds that allow the electrical stimulation of Schwann cells cultured on the scaffolds which increases the production of nerve growth factor (NGF). |
| first_indexed | 2024-03-12T07:09:49Z |
| format | Article |
| id | doaj.art-0d0bc6b003934491adaa657a1c134693 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T07:09:49Z |
| publishDate | 2015-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-0d0bc6b003934491adaa657a1c1346932023-09-02T23:08:18ZengMDPI AGBioengineering2306-53542015-01-0121153410.3390/bioengineering2010015bioengineering2010015Electroactive Tissue Scaffolds with Aligned Pores as Instructive Platforms for Biomimetic Tissue EngineeringJohn G. Hardy0R. Chase Cornelison1Rushi C. Sukhavasi2Richard J. Saballos3Philip Vu4David L. Kaplan5Christine E. Schmidt6Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USADepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USADepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USACrayton Pruitt Family Department of Biomedical Engineering, University of Florida, Biomedical Sciences Building JG-53, P.O. Box 116131, Gainesville, FL 32611, USACrayton Pruitt Family Department of Biomedical Engineering, University of Florida, Biomedical Sciences Building JG-53, P.O. Box 116131, Gainesville, FL 32611, USADepartment of Biomedical Engineering, Tufts University, Medford, MA 02155, USADepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USATissues in the body are hierarchically structured composite materials with tissue-specific chemical and topographical properties. Here we report the preparation of tissue scaffolds with macroscopic pores generated via the dissolution of a sacrificial supramolecular polymer-based crystal template (urea) from a biodegradable polymer-based scaffold (polycaprolactone, PCL). Furthermore, we report a method of aligning the supramolecular polymer-based crystals within the PCL, and that the dissolution of the sacrificial urea yields scaffolds with macroscopic pores that are aligned over long, clinically-relevant distances (i.e., centimeter scale). The pores act as topographical cues to which rat Schwann cells respond by aligning with the long axis of the pores. Generation of an interpenetrating network of polypyrrole (PPy) and poly(styrene sulfonate) (PSS) in the scaffolds yields electroactive tissue scaffolds that allow the electrical stimulation of Schwann cells cultured on the scaffolds which increases the production of nerve growth factor (NGF).http://www.mdpi.com/2306-5354/2/1/15electroactive polymersmicrofabricationnerve guideperipheral nerveplastic electronicstopography |
| spellingShingle | John G. Hardy R. Chase Cornelison Rushi C. Sukhavasi Richard J. Saballos Philip Vu David L. Kaplan Christine E. Schmidt Electroactive Tissue Scaffolds with Aligned Pores as Instructive Platforms for Biomimetic Tissue Engineering Bioengineering electroactive polymers microfabrication nerve guide peripheral nerve plastic electronics topography |
| title | Electroactive Tissue Scaffolds with Aligned Pores as Instructive Platforms for Biomimetic Tissue Engineering |
| title_full | Electroactive Tissue Scaffolds with Aligned Pores as Instructive Platforms for Biomimetic Tissue Engineering |
| title_fullStr | Electroactive Tissue Scaffolds with Aligned Pores as Instructive Platforms for Biomimetic Tissue Engineering |
| title_full_unstemmed | Electroactive Tissue Scaffolds with Aligned Pores as Instructive Platforms for Biomimetic Tissue Engineering |
| title_short | Electroactive Tissue Scaffolds with Aligned Pores as Instructive Platforms for Biomimetic Tissue Engineering |
| title_sort | electroactive tissue scaffolds with aligned pores as instructive platforms for biomimetic tissue engineering |
| topic | electroactive polymers microfabrication nerve guide peripheral nerve plastic electronics topography |
| url | http://www.mdpi.com/2306-5354/2/1/15 |
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