Quantitative Proteomics Reveals Significant Differences between Mouse Brain Formations in Expression of Proteins Involved in Neuronal Plasticity during Aging
Aging is associated with a general decline in cognitive functions, which appears to be due to alterations in the amounts of proteins involved in the regulation of synaptic plasticity. Here, we present a quantitative analysis of proteins involved in neurotransmission in three brain regions, namely, t...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2073-4409/10/8/2021 |
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| author | Dominika Drulis-Fajdasz Kinga Gostomska-Pampuch Przemysław Duda Jacek Roman Wiśniewski Dariusz Rakus |
| author_facet | Dominika Drulis-Fajdasz Kinga Gostomska-Pampuch Przemysław Duda Jacek Roman Wiśniewski Dariusz Rakus |
| author_sort | Dominika Drulis-Fajdasz |
| collection | DOAJ |
| description | Aging is associated with a general decline in cognitive functions, which appears to be due to alterations in the amounts of proteins involved in the regulation of synaptic plasticity. Here, we present a quantitative analysis of proteins involved in neurotransmission in three brain regions, namely, the hippocampus, the cerebral cortex and the cerebellum, in mice aged 1 and 22 months, using the total protein approach technique. We demonstrate that although the titer of some proteins involved in neurotransmission and synaptic plasticity is affected by aging in a similar manner in all the studied brain formations, in fact, each of the formations represents its own mode of aging. Generally, the hippocampal and cortical proteomes are much more unstable during the lifetime than the cerebellar proteome. The data presented here provide a general picture of the effect of physiological aging on synaptic plasticity and might suggest potential drug targets for anti-aging therapies. |
| first_indexed | 2024-03-10T08:55:10Z |
| format | Article |
| id | doaj.art-0d0edc8c98154c3ba79f83e1c20b9aac |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T08:55:10Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-0d0edc8c98154c3ba79f83e1c20b9aac2023-11-22T07:10:36ZengMDPI AGCells2073-44092021-08-01108202110.3390/cells10082021Quantitative Proteomics Reveals Significant Differences between Mouse Brain Formations in Expression of Proteins Involved in Neuronal Plasticity during AgingDominika Drulis-Fajdasz0Kinga Gostomska-Pampuch1Przemysław Duda2Jacek Roman Wiśniewski3Dariusz Rakus4Department of Molecular Physiology and Neurobiology, University of Wrocław, Sienkiewicza 21, 50-335 Wrocław, PolandBiochemical Proteomics Group, Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, GermanyDepartment of Molecular Physiology and Neurobiology, University of Wrocław, Sienkiewicza 21, 50-335 Wrocław, PolandBiochemical Proteomics Group, Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, GermanyDepartment of Molecular Physiology and Neurobiology, University of Wrocław, Sienkiewicza 21, 50-335 Wrocław, PolandAging is associated with a general decline in cognitive functions, which appears to be due to alterations in the amounts of proteins involved in the regulation of synaptic plasticity. Here, we present a quantitative analysis of proteins involved in neurotransmission in three brain regions, namely, the hippocampus, the cerebral cortex and the cerebellum, in mice aged 1 and 22 months, using the total protein approach technique. We demonstrate that although the titer of some proteins involved in neurotransmission and synaptic plasticity is affected by aging in a similar manner in all the studied brain formations, in fact, each of the formations represents its own mode of aging. Generally, the hippocampal and cortical proteomes are much more unstable during the lifetime than the cerebellar proteome. The data presented here provide a general picture of the effect of physiological aging on synaptic plasticity and might suggest potential drug targets for anti-aging therapies.https://www.mdpi.com/2073-4409/10/8/2021glutamatergic and GABAergic transmissionCamk2OXPHOSextracellular matrixtotal protein approachhippocampus |
| spellingShingle | Dominika Drulis-Fajdasz Kinga Gostomska-Pampuch Przemysław Duda Jacek Roman Wiśniewski Dariusz Rakus Quantitative Proteomics Reveals Significant Differences between Mouse Brain Formations in Expression of Proteins Involved in Neuronal Plasticity during Aging Cells glutamatergic and GABAergic transmission Camk2 OXPHOS extracellular matrix total protein approach hippocampus |
| title | Quantitative Proteomics Reveals Significant Differences between Mouse Brain Formations in Expression of Proteins Involved in Neuronal Plasticity during Aging |
| title_full | Quantitative Proteomics Reveals Significant Differences between Mouse Brain Formations in Expression of Proteins Involved in Neuronal Plasticity during Aging |
| title_fullStr | Quantitative Proteomics Reveals Significant Differences between Mouse Brain Formations in Expression of Proteins Involved in Neuronal Plasticity during Aging |
| title_full_unstemmed | Quantitative Proteomics Reveals Significant Differences between Mouse Brain Formations in Expression of Proteins Involved in Neuronal Plasticity during Aging |
| title_short | Quantitative Proteomics Reveals Significant Differences between Mouse Brain Formations in Expression of Proteins Involved in Neuronal Plasticity during Aging |
| title_sort | quantitative proteomics reveals significant differences between mouse brain formations in expression of proteins involved in neuronal plasticity during aging |
| topic | glutamatergic and GABAergic transmission Camk2 OXPHOS extracellular matrix total protein approach hippocampus |
| url | https://www.mdpi.com/2073-4409/10/8/2021 |
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