T4-related bacteriophage LIMEstone isolates for the control of soft rot on potato caused by 'Dickeya solani'.
The bacterium 'Dickeya solani', an aggressive biovar 3 variant of Dickeya dianthicola, causes rotting and blackleg in potato. To control this pathogen using bacteriophage therapy, we isolated and characterized two closely related and specific bacteriophages, vB_DsoM_LIMEstone1 and vB_DsoM_...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3296691?pdf=render |
| _version_ | 1811259451149123584 |
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| author | Evelien M Adriaenssens Johan Van Vaerenbergh Dieter Vandenheuvel Vincent Dunon Pieter-Jan Ceyssens Maurice De Proft Andrew M Kropinski Jean-Paul Noben Martine Maes Rob Lavigne |
| author_facet | Evelien M Adriaenssens Johan Van Vaerenbergh Dieter Vandenheuvel Vincent Dunon Pieter-Jan Ceyssens Maurice De Proft Andrew M Kropinski Jean-Paul Noben Martine Maes Rob Lavigne |
| author_sort | Evelien M Adriaenssens |
| collection | DOAJ |
| description | The bacterium 'Dickeya solani', an aggressive biovar 3 variant of Dickeya dianthicola, causes rotting and blackleg in potato. To control this pathogen using bacteriophage therapy, we isolated and characterized two closely related and specific bacteriophages, vB_DsoM_LIMEstone1 and vB_DsoM_LIMEstone2. The LIMEstone phages have a T4-related genome organization and share DNA similarity with Salmonella phage ViI. Microbiological and molecular characterization of the phages deemed them suitable and promising for use in phage therapy. The phages reduced disease incidence and severity on potato tubers in laboratory assays. In addition, in a field trial of potato tubers, when infected with 'Dickeya solani', the experimental phage treatment resulted in a higher yield. These results form the basis for the development of a bacteriophage-based biocontrol of potato plants and tubers as an alternative for the use of antibiotics. |
| first_indexed | 2024-04-12T18:31:43Z |
| format | Article |
| id | doaj.art-0d105a49666048f39158bb0eb9760bfb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T18:31:43Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0d105a49666048f39158bb0eb9760bfb2022-12-22T03:21:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0173e3322710.1371/journal.pone.0033227T4-related bacteriophage LIMEstone isolates for the control of soft rot on potato caused by 'Dickeya solani'.Evelien M AdriaenssensJohan Van VaerenberghDieter VandenheuvelVincent DunonPieter-Jan CeyssensMaurice De ProftAndrew M KropinskiJean-Paul NobenMartine MaesRob LavigneThe bacterium 'Dickeya solani', an aggressive biovar 3 variant of Dickeya dianthicola, causes rotting and blackleg in potato. To control this pathogen using bacteriophage therapy, we isolated and characterized two closely related and specific bacteriophages, vB_DsoM_LIMEstone1 and vB_DsoM_LIMEstone2. The LIMEstone phages have a T4-related genome organization and share DNA similarity with Salmonella phage ViI. Microbiological and molecular characterization of the phages deemed them suitable and promising for use in phage therapy. The phages reduced disease incidence and severity on potato tubers in laboratory assays. In addition, in a field trial of potato tubers, when infected with 'Dickeya solani', the experimental phage treatment resulted in a higher yield. These results form the basis for the development of a bacteriophage-based biocontrol of potato plants and tubers as an alternative for the use of antibiotics.http://europepmc.org/articles/PMC3296691?pdf=render |
| spellingShingle | Evelien M Adriaenssens Johan Van Vaerenbergh Dieter Vandenheuvel Vincent Dunon Pieter-Jan Ceyssens Maurice De Proft Andrew M Kropinski Jean-Paul Noben Martine Maes Rob Lavigne T4-related bacteriophage LIMEstone isolates for the control of soft rot on potato caused by 'Dickeya solani'. PLoS ONE |
| title | T4-related bacteriophage LIMEstone isolates for the control of soft rot on potato caused by 'Dickeya solani'. |
| title_full | T4-related bacteriophage LIMEstone isolates for the control of soft rot on potato caused by 'Dickeya solani'. |
| title_fullStr | T4-related bacteriophage LIMEstone isolates for the control of soft rot on potato caused by 'Dickeya solani'. |
| title_full_unstemmed | T4-related bacteriophage LIMEstone isolates for the control of soft rot on potato caused by 'Dickeya solani'. |
| title_short | T4-related bacteriophage LIMEstone isolates for the control of soft rot on potato caused by 'Dickeya solani'. |
| title_sort | t4 related bacteriophage limestone isolates for the control of soft rot on potato caused by dickeya solani |
| url | http://europepmc.org/articles/PMC3296691?pdf=render |
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