Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation
Gold nanoparticles (AuNPs) exhibit unique properties that can be modulated through a tailored surface functionalization, enabling their targeted use in biochemical sensing and medical diagnostics. In particular, streptavidin-modified AuNPs are increasingly used for biosensing purposes. We report her...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Beilstein-Institut
2017-01-01
|
| Series: | Beilstein Journal of Nanotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.3762/bjnano.8.1 |
| _version_ | 1798043179950276608 |
|---|---|
| author | Roberta D'Agata Pasquale Palladino Giuseppe Spoto |
| author_facet | Roberta D'Agata Pasquale Palladino Giuseppe Spoto |
| author_sort | Roberta D'Agata |
| collection | DOAJ |
| description | Gold nanoparticles (AuNPs) exhibit unique properties that can be modulated through a tailored surface functionalization, enabling their targeted use in biochemical sensing and medical diagnostics. In particular, streptavidin-modified AuNPs are increasingly used for biosensing purposes. We report here a study of AuNPs surface-functionalized with streptavidin-biotinylated oligonucleotide, focussing on the role played by the oligonucleotide probes in the stabilization/destabilization of the functionalized nanoparticle dispersion. The behaviour of the modified AuNP dispersion as a consequence of the competitive displacement of the biotinylated oligonucleotide has been investigated and the critical role of displaced oligonucletides in triggering the quasi one-dimensional aggregation of nanoparticles is demonstrated for the first time. The thorough understanding of the fundamental properties of bioconjugated AuNPs is of great importance for the design of highly sensitive and reliable functionalized AuNP-based assays. |
| first_indexed | 2024-04-11T22:45:54Z |
| format | Article |
| id | doaj.art-0d10980f029c49c090c5fb105f48dc8c |
| institution | Directory Open Access Journal |
| issn | 2190-4286 |
| language | English |
| last_indexed | 2024-04-11T22:45:54Z |
| publishDate | 2017-01-01 |
| publisher | Beilstein-Institut |
| record_format | Article |
| series | Beilstein Journal of Nanotechnology |
| spelling | doaj.art-0d10980f029c49c090c5fb105f48dc8c2022-12-22T03:58:46ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862017-01-018111110.3762/bjnano.8.12190-4286-8-1Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregationRoberta D'Agata0Pasquale Palladino1Giuseppe Spoto2Consorio Interuniversitario “Istituto Nazionale Biostrutture e Biosistemi”, c/o Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, I-95125, Catania, ItalyConsorio Interuniversitario “Istituto Nazionale Biostrutture e Biosistemi”, c/o Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, I-95125, Catania, ItalyConsorio Interuniversitario “Istituto Nazionale Biostrutture e Biosistemi”, c/o Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, I-95125, Catania, ItalyGold nanoparticles (AuNPs) exhibit unique properties that can be modulated through a tailored surface functionalization, enabling their targeted use in biochemical sensing and medical diagnostics. In particular, streptavidin-modified AuNPs are increasingly used for biosensing purposes. We report here a study of AuNPs surface-functionalized with streptavidin-biotinylated oligonucleotide, focussing on the role played by the oligonucleotide probes in the stabilization/destabilization of the functionalized nanoparticle dispersion. The behaviour of the modified AuNP dispersion as a consequence of the competitive displacement of the biotinylated oligonucleotide has been investigated and the critical role of displaced oligonucletides in triggering the quasi one-dimensional aggregation of nanoparticles is demonstrated for the first time. The thorough understanding of the fundamental properties of bioconjugated AuNPs is of great importance for the design of highly sensitive and reliable functionalized AuNP-based assays.https://doi.org/10.3762/bjnano.8.1biosensorsDNAgold nanoparticlesnanoparticles aggregationplasmonicsstreptavidin |
| spellingShingle | Roberta D'Agata Pasquale Palladino Giuseppe Spoto Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation Beilstein Journal of Nanotechnology biosensors DNA gold nanoparticles nanoparticles aggregation plasmonics streptavidin |
| title | Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation |
| title_full | Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation |
| title_fullStr | Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation |
| title_full_unstemmed | Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation |
| title_short | Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation |
| title_sort | streptavidin coated gold nanoparticles critical role of oligonucleotides on stability and fractal aggregation |
| topic | biosensors DNA gold nanoparticles nanoparticles aggregation plasmonics streptavidin |
| url | https://doi.org/10.3762/bjnano.8.1 |
| work_keys_str_mv | AT robertadagata streptavidincoatedgoldnanoparticlescriticalroleofoligonucleotidesonstabilityandfractalaggregation AT pasqualepalladino streptavidincoatedgoldnanoparticlescriticalroleofoligonucleotidesonstabilityandfractalaggregation AT giuseppespoto streptavidincoatedgoldnanoparticlescriticalroleofoligonucleotidesonstabilityandfractalaggregation |