Cytotoxicity, antibacterial and antifungal activities of ZnO nanoparticles prepared by the Artocarpus gomezianus fruit mediated facile green combustion method
Spherical nanoparticles of zinc oxide (ZnO NPs) were synthesized by an eco-friendly green combustion method using citrate containing Artocarpus gomezianus fruit extract as a fuel. The morphology, compositions and structure of the product were characterized by Powder X-ray Diffraction (PXRD), Scannin...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-12-01
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| Series: | Journal of Science: Advanced Materials and Devices |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468217918301485 |
| _version_ | 1819230078496669696 |
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| author | R. Anitha K.V. Ramesh T.N. Ravishankar K.H. Sudheer Kumar T. Ramakrishnappa |
| author_facet | R. Anitha K.V. Ramesh T.N. Ravishankar K.H. Sudheer Kumar T. Ramakrishnappa |
| author_sort | R. Anitha |
| collection | DOAJ |
| description | Spherical nanoparticles of zinc oxide (ZnO NPs) were synthesized by an eco-friendly green combustion method using citrate containing Artocarpus gomezianus fruit extract as a fuel. The morphology, compositions and structure of the product were characterized by Powder X-ray Diffraction (PXRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Fourier Transform Infra-red (FTIR), UV–Visible (UV–Vis) and Raman Spectroscopy. Highly uniform spherical zinc oxide NPs were subjected to cytotoxicity, antifungal and antibacterial activities. PXRD patterns show that the product formed belongs to a hexagonal wurtzite system. SEM micrographs reveal that the particles are agglomerated. The TEM images demonstrate that the particles are highly uniform spherical in shape and loosely agglomerated. Scherrer's method and WH plots were used to calculate the average crystallite sizes, yielding 39, 35, 31 and 40, 37, 32 nm for ZnO NPs prepared with 5, 10 and 15 mL of 10% Artocarpus gomezianus fruit extract, respectively. These results were confirmed by the TEM observations. Breast cancer cell lines (MCF-7) were subjected to in vitro anticancer activity. MTT assay revealed a good anticancer activity of ZnO NPs against MCF-7. Zone of the inhibition method shows that the spherical ZnO NPs also exhibit significant antibacterial activity against staphylococcus aureus and antifungal activity against Aspergillus niger. The synthesized ZnO NPs can find plausible biological applications. Keywords: Green synthesis, ZnO nanoparticles, Anticancer activity, MCF-7, Antibacterial, Antifungal |
| first_indexed | 2024-12-23T11:23:21Z |
| format | Article |
| id | doaj.art-0d11b10e0f784fe19b7c6ae9be67d3e6 |
| institution | Directory Open Access Journal |
| issn | 2468-2179 |
| language | English |
| last_indexed | 2024-12-23T11:23:21Z |
| publishDate | 2018-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Science: Advanced Materials and Devices |
| spelling | doaj.art-0d11b10e0f784fe19b7c6ae9be67d3e62022-12-21T17:49:01ZengElsevierJournal of Science: Advanced Materials and Devices2468-21792018-12-0134440451Cytotoxicity, antibacterial and antifungal activities of ZnO nanoparticles prepared by the Artocarpus gomezianus fruit mediated facile green combustion methodR. Anitha0K.V. Ramesh1T.N. Ravishankar2K.H. Sudheer Kumar3T. Ramakrishnappa4Department of Biochemistry, Bharathiar University, Coimbatore, 641 046, IndiaPG Department of Biochemistry, Dayananda Sagar College, Bangalore, 560 078, IndiaDepartment of Chemistry, Global Academy of Technology (GAT), Rajarajeshwarinagar, Off Mysore Road, Ideal Homes Township, Bangalore, 560098, Karnataka, IndiaDepartment of Chemistry, BMS Institute of Technology and Management, Avalahalli, Doddaballapura Main Road, Yelahanka, Bangalore, 560064, IndiaDepartment of Chemistry, BMS Institute of Technology and Management, Avalahalli, Doddaballapura Main Road, Yelahanka, Bangalore, 560064, India; Corresponding author.Spherical nanoparticles of zinc oxide (ZnO NPs) were synthesized by an eco-friendly green combustion method using citrate containing Artocarpus gomezianus fruit extract as a fuel. The morphology, compositions and structure of the product were characterized by Powder X-ray Diffraction (PXRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Fourier Transform Infra-red (FTIR), UV–Visible (UV–Vis) and Raman Spectroscopy. Highly uniform spherical zinc oxide NPs were subjected to cytotoxicity, antifungal and antibacterial activities. PXRD patterns show that the product formed belongs to a hexagonal wurtzite system. SEM micrographs reveal that the particles are agglomerated. The TEM images demonstrate that the particles are highly uniform spherical in shape and loosely agglomerated. Scherrer's method and WH plots were used to calculate the average crystallite sizes, yielding 39, 35, 31 and 40, 37, 32 nm for ZnO NPs prepared with 5, 10 and 15 mL of 10% Artocarpus gomezianus fruit extract, respectively. These results were confirmed by the TEM observations. Breast cancer cell lines (MCF-7) were subjected to in vitro anticancer activity. MTT assay revealed a good anticancer activity of ZnO NPs against MCF-7. Zone of the inhibition method shows that the spherical ZnO NPs also exhibit significant antibacterial activity against staphylococcus aureus and antifungal activity against Aspergillus niger. The synthesized ZnO NPs can find plausible biological applications. Keywords: Green synthesis, ZnO nanoparticles, Anticancer activity, MCF-7, Antibacterial, Antifungalhttp://www.sciencedirect.com/science/article/pii/S2468217918301485 |
| spellingShingle | R. Anitha K.V. Ramesh T.N. Ravishankar K.H. Sudheer Kumar T. Ramakrishnappa Cytotoxicity, antibacterial and antifungal activities of ZnO nanoparticles prepared by the Artocarpus gomezianus fruit mediated facile green combustion method Journal of Science: Advanced Materials and Devices |
| title | Cytotoxicity, antibacterial and antifungal activities of ZnO nanoparticles prepared by the Artocarpus gomezianus fruit mediated facile green combustion method |
| title_full | Cytotoxicity, antibacterial and antifungal activities of ZnO nanoparticles prepared by the Artocarpus gomezianus fruit mediated facile green combustion method |
| title_fullStr | Cytotoxicity, antibacterial and antifungal activities of ZnO nanoparticles prepared by the Artocarpus gomezianus fruit mediated facile green combustion method |
| title_full_unstemmed | Cytotoxicity, antibacterial and antifungal activities of ZnO nanoparticles prepared by the Artocarpus gomezianus fruit mediated facile green combustion method |
| title_short | Cytotoxicity, antibacterial and antifungal activities of ZnO nanoparticles prepared by the Artocarpus gomezianus fruit mediated facile green combustion method |
| title_sort | cytotoxicity antibacterial and antifungal activities of zno nanoparticles prepared by the artocarpus gomezianus fruit mediated facile green combustion method |
| url | http://www.sciencedirect.com/science/article/pii/S2468217918301485 |
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