TGase介导玉米醇溶蛋白改善 大豆分离蛋白膜性能的研究TGase-induced zein to improve properties of soy protein isolates film
为改善大豆分离蛋白膜的性能,利用碱性蛋白酶制备玉米醇溶蛋白酶解产物(ZH),通过转谷氨酰胺酶(TGase)催化大豆分离蛋白(SPI)与ZH发生交联反应,对反应条件进行了优化;通过控制反应时间得到4种修饰产物(分别为0 h-SPI/ZH、0.5 h-SPI/ZH、1 h-SPI/ZH、1.5 h-SPI/ZH),并对修饰产物制备的膜性能进行研究。结果表明:最佳的TGase催化SPI与ZH交联反应条件为SPI与ZH质量比3.5∶ 1、底物质量浓度40 mg/mL、pH 7.5、酶添加量10 U/g(以蛋白质质量计)、反应温度45 ℃、反应时间1.5 h;随着反应时间的延长,4种修饰产物制备的复合膜...
Main Author: | 曹芷郡1,2,3,陈佳鹏1,任健1,2,3,宋春丽1,2,3,刘军3 CAO Zhijun1,2,3, CHEN Jiapeng1, REN Jian1,2,3, SONG Chunli1,2,3, LIU Jun3 |
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Format: | Article |
Language: | English |
Published: |
中粮工科(西安)国际工程有限公司
2023-07-01
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Series: | Zhongguo youzhi |
Subjects: | |
Online Access: | http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20230714&flag=1 |
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