Synthesis and functionalization of silver ferrite (AgFe2O3) nanoparticles with l-methionine: In vivo toxicity studies against Drosophila melanogaster (Diptera: Drosophilidae)
In the present study, silver ferrite nanoparticles (AgFe2O3 NPs) were synthesized by a simple and rapid solution precipitation method using ammonium hydroxide as a complexing agent. AgFe2O3 NPs were conjugated with folic acid and l-methionine for in vivo administration to Drosophila melanogaster (DM...
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| Format: | Article |
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Elsevier
2022-01-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715622002843 |
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| author | D.M. Surendra N.A. Chamaraja S.S. Godipurge S. Yallappa |
| author_facet | D.M. Surendra N.A. Chamaraja S.S. Godipurge S. Yallappa |
| author_sort | D.M. Surendra |
| collection | DOAJ |
| description | In the present study, silver ferrite nanoparticles (AgFe2O3 NPs) were synthesized by a simple and rapid solution precipitation method using ammonium hydroxide as a complexing agent. AgFe2O3 NPs were conjugated with folic acid and l-methionine for in vivo administration to Drosophila melanogaster (DM) flies. The drug conjugated AgFe2O3 NPs were characterized by using different physicochemical techniques such as UV–Visible spectrophotometer, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscope (SEM), particle size distribution (DLS), energy dispersive X-ray (EDX) and thermogravimetric analysis. SEM images reveal the nearly spherical shaped AgFe2O3 NPs (ф 25–30 nm), while energy dispersive X-ray spectroscopy reveals the expected elemental composition. On the DM flies’ model (observation from 3 to 50 days), the results reveal that drug conjugated AgFe2O3 NPs have good repeatability and are less toxic. Furthermore, functionalized NPs of concentrations 0.25 mM, 0.5 mM, and 1 mM were administered orally, but no significant differences in viability, fecundity, or locomotory assay were identified up to 0.5 mM. The drug loading and releasing efficiency were tested, and the amount of drug released was highest at pH 5.3 (the normal ambient setting for intravenous neuron drugs), followed by pH 7.2 and pH 6.8. This study showed that the functionalized AgFe2O3 NPs are safe to use in living organisms. As a result, they can be used as a drug delivery carrier in many disease models, including neural delivery. |
| first_indexed | 2024-04-11T12:52:29Z |
| format | Article |
| id | doaj.art-0d1666f760ce4c3a81ba56a38779ce46 |
| institution | Directory Open Access Journal |
| issn | 2211-7156 |
| language | English |
| last_indexed | 2024-04-11T12:52:29Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj.art-0d1666f760ce4c3a81ba56a38779ce462022-12-22T04:23:10ZengElsevierResults in Chemistry2211-71562022-01-014100565Synthesis and functionalization of silver ferrite (AgFe2O3) nanoparticles with l-methionine: In vivo toxicity studies against Drosophila melanogaster (Diptera: Drosophilidae)D.M. Surendra0N.A. Chamaraja1S.S. Godipurge2S. Yallappa3Department of Chemistry, Vidya Vikas Institute of Engineering and Technology, Mysuru, Karnataka, IndiaDepartment of Chemistry, JSS Academy of Technical Education, Dr. Vishnuvardhan Road, Bengaluru 560060, Karnataka, India; Corresponding authors at: Department of Chemistry, JSS Academy of Technical Education, Dr. Vishnuvardhan Road, Bengaluru 560060, Karnataka, India (N.A. Chamaraja). Department of Chemistry, AMC Engineering College, Bannerghatta Road, Bangalore 560083, Karnataka, India (S. Yallappa).Department of P. G. Studies and Research in Chemistry, Gulbarga University, Kalaburagi 585 106, Karnataka, IndiaDepartment of Chemistry, AMC Engineering College, Bannerghatta Road, Bangalore 560083, Karnataka, India; Department of Physical Science and Materials Engineering Faculty of Science and Engineering, Iwate University, Morioka 020-8551, Japan; Corresponding authors at: Department of Chemistry, JSS Academy of Technical Education, Dr. Vishnuvardhan Road, Bengaluru 560060, Karnataka, India (N.A. Chamaraja). Department of Chemistry, AMC Engineering College, Bannerghatta Road, Bangalore 560083, Karnataka, India (S. Yallappa).In the present study, silver ferrite nanoparticles (AgFe2O3 NPs) were synthesized by a simple and rapid solution precipitation method using ammonium hydroxide as a complexing agent. AgFe2O3 NPs were conjugated with folic acid and l-methionine for in vivo administration to Drosophila melanogaster (DM) flies. The drug conjugated AgFe2O3 NPs were characterized by using different physicochemical techniques such as UV–Visible spectrophotometer, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscope (SEM), particle size distribution (DLS), energy dispersive X-ray (EDX) and thermogravimetric analysis. SEM images reveal the nearly spherical shaped AgFe2O3 NPs (ф 25–30 nm), while energy dispersive X-ray spectroscopy reveals the expected elemental composition. On the DM flies’ model (observation from 3 to 50 days), the results reveal that drug conjugated AgFe2O3 NPs have good repeatability and are less toxic. Furthermore, functionalized NPs of concentrations 0.25 mM, 0.5 mM, and 1 mM were administered orally, but no significant differences in viability, fecundity, or locomotory assay were identified up to 0.5 mM. The drug loading and releasing efficiency were tested, and the amount of drug released was highest at pH 5.3 (the normal ambient setting for intravenous neuron drugs), followed by pH 7.2 and pH 6.8. This study showed that the functionalized AgFe2O3 NPs are safe to use in living organisms. As a result, they can be used as a drug delivery carrier in many disease models, including neural delivery.http://www.sciencedirect.com/science/article/pii/S2211715622002843AgFe2O3 nanoparticlesSEMl-MethionineFolic acidDrosophila melanogasterIn vivo toxicity |
| spellingShingle | D.M. Surendra N.A. Chamaraja S.S. Godipurge S. Yallappa Synthesis and functionalization of silver ferrite (AgFe2O3) nanoparticles with l-methionine: In vivo toxicity studies against Drosophila melanogaster (Diptera: Drosophilidae) Results in Chemistry AgFe2O3 nanoparticles SEM l-Methionine Folic acid Drosophila melanogaster In vivo toxicity |
| title | Synthesis and functionalization of silver ferrite (AgFe2O3) nanoparticles with l-methionine: In vivo toxicity studies against Drosophila melanogaster (Diptera: Drosophilidae) |
| title_full | Synthesis and functionalization of silver ferrite (AgFe2O3) nanoparticles with l-methionine: In vivo toxicity studies against Drosophila melanogaster (Diptera: Drosophilidae) |
| title_fullStr | Synthesis and functionalization of silver ferrite (AgFe2O3) nanoparticles with l-methionine: In vivo toxicity studies against Drosophila melanogaster (Diptera: Drosophilidae) |
| title_full_unstemmed | Synthesis and functionalization of silver ferrite (AgFe2O3) nanoparticles with l-methionine: In vivo toxicity studies against Drosophila melanogaster (Diptera: Drosophilidae) |
| title_short | Synthesis and functionalization of silver ferrite (AgFe2O3) nanoparticles with l-methionine: In vivo toxicity studies against Drosophila melanogaster (Diptera: Drosophilidae) |
| title_sort | synthesis and functionalization of silver ferrite agfe2o3 nanoparticles with l methionine in vivo toxicity studies against drosophila melanogaster diptera drosophilidae |
| topic | AgFe2O3 nanoparticles SEM l-Methionine Folic acid Drosophila melanogaster In vivo toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2211715622002843 |
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